Prithipal Singh
Banaras Hindu University
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Publication
Featured researches published by Prithipal Singh.
Analytical Biochemistry | 1980
Prithipal Singh; Danton K. Leung; Gerald L. Rowley; Carmen Gagne; Edwin F. Ullman
Abstract A new method for coupling of amino compounds to enzymes is described. An aminoglycoside, gentamicin, is functionalized with a nucleophilic sulfhydryl group on its deoxystreptamine ring and an enzyme, glucose 6-phosphate dehydrogenase (G6PDH), is modified with electrophilic bromoacetylglycyl groups. Upon modification, the G6PDH loses about 70% of its enzyme activity. Coupling of the modified G6PDH with the sulfhydryl-substituted gentamicin affords, without further loss in activity, the drug-enzyme conjugate. Gentamicin antibodies inhibit up to 76% of the enzyme activity of the conjugate. The conjugate is demonstrated to be useful for a sensitive, homogeneous enzyme immunoassay for gentamicin in human serum.
Journal of the American Chemical Society | 1972
Edwin F. Ullman; Prithipal Singh
Archive | 1986
Christine G. Collins; Marcel R. Pirio; Prithipal Singh
Canadian Journal of Chemistry | 1984
Prithipal Singh; Marcel R. Pirio; Danton K. Leung; Yuh-Geng Tsay
Archive | 1978
Prithipal Singh; Marcel R. Pirio
Journal of the American Chemical Society | 1976
Prithipal Singh; Edwin F. Ullman
Archive | 1979
Prithipal Singh; Marcel R. Pirio
Archive | 1983
Prithipal Singh; Danton K. Leung; Richard Rodgers
Archive | 1980
Gerald L. Rowley; Danton K. Leung; Prithipal Singh
Journal of Organic Chemistry | 1980
Mae W. Hu; Prithipal Singh; Edwin F. Ullman
