Priyanka Debta

Evaluation of Prognostic Significance of Immunological Cells (Tissue Eosinophil and Mast Cell) Infiltration in Oral Squamous Cell Carcinoma

Background: In oral carcinoma much effort has been made to predict the prognosis of patients but a sound understanding of underlying cell biology is likely to need progress. Recently, attention has been directed towards tumour associated tissue eosinophils and mast cells and their role in the biologic behavior of tumours. Aim: The retrospective study was used to evaluate the influence of tumour associated tissue eosinophils and mast cells on prognosis of oral squamous cell carcinoma (OSCC). Material and methods: The follow-up, of histopathologically diagnosed thirty cases of OSCC, was carried out for minimum period of 3 years. Special stains are wonderful they allow us to see which we can not see clear with routine H&E stain.Tissue sections were stained with special stains, Carbol Chromotrope for tissue eosinophil and Toluidine blue for tissue mast cell staining. Result: The results of the present study shows that increase infiltration of tissue eosinophils and mast cells in OSCC, associated with favourable prognosis. Conclusion: We concludes that infiltration of tissue eosinophils and mast cells are indicators of favourable prognosis in OSCC. Thus quantitative assessment of eosinophils and mast cells are the most important aspects of the microscopic evaluation of OSCC.

Evaluation of Infiltration of Immunological Cells (Tissue Eosinophil and Mast Cell) in Odontogenic Cysts by Using Special Stains

Cells of immune system comprised of lymphoid series and myeloid progenitor series cells. Mast cells and tissue eosinophils both are granulocytes which come under myeloid progenitor series of immune cells system. The presence of mast cells in odontogenic cyst could contribute to their pathogenesis in several ways. Mast cells liberate ECF (Eosinophils chemoattractant factor) and histamine, which attract eosinophils in tissue. Mast cells and eosinophils both have also been implicated in stimulating the production of prostaglandins, important in bone resorption for odontogenic cyst growth. The mast cells and eosinophils are features of both keratinizing and non-keratinizing cysts (OKC, DC, RC) but their number does not necessarily correlate with degree of inflammation. Special stains to demonstrate mast cells and eosinophils were used. Carbol Chromotrope staining method to demonstrate tissue eosinophil was found to be better than Congo red. The staining intensity for mast cells was equally good with thionin and toluidine blue.

Evaluation of myeloid cells (tumor-associated tissue eosinophils and mast cells) infiltration in different grades of oral squamous cell carcinoma

Background: The multifunctional involvement and infiltration of myeloid cells (tumor-associated tissue eosinophils [TATE] and mast cells) can provide a unique opportunity to define relevant effectors functions that may represent novel, therapeutic options for modulation of tumor onset/growth. Aim: Our study aimed to evaluate infiltration of myeloid cells (TATE and Mast cells) infiltration in different grades (WHO grading) of oral squamous cell carcinoma (OSCC). Materials and Methods: Total 30 cases of OSCC were selected for this study. Hematoxylin and eosin stain and toluidine blue special stain, to evaluate TATE and the mast cells infiltration, were used. Three-year follow-up of OSCC cases was done. Result: Among 30 cases, 63.33% cases of OSCC showed TATE-positive and 36.66% cases showed TATE-negative. Regarding mast cells infiltration, 66.66% OSCC cases showed mast cells positive and 33.33% cases did not show significant mast cells infiltration. We found significant association of TATE and mast cells infiltration in OSCC cases. These myeloid cells infiltration significantly associated with age of patients but did not show any significant association with gender, site, and habit of cases. When we compared these cells infiltration with clinical stages and different histological grades of tumor, we found their infiltration is decreasing, from Stages 1 to Stage 3 of tumor and from well to poorly differentiated carcinoma. We have also found the less infiltration of these myeloid in recurrence cases of OSCC. Conclusion: As the infiltration of TATE and mast cells are correlated, along with evaluation of TATE, we should also evaluate the presence of mast cells infiltration in OSCC. The assessment of myeloid cells could become, in the future, useful for therapeutic approaches in this subset of the patient.

Evaluation of infiltration of immunological cells (tumour associated tissue eosinophils and mast cells) in oral squamous cell carcinoma by using special stains.

Aim: Our study aimed to evaluate the infiltration of tissue eosinophils and mast cells in oral squamous cell carcinoma (OSCC) by using special stains. Study Design: Comparative study. Place of Study : Sharad Pawar Dental College, Sawangi (Meghe), Wardha, Maharashtra. Methodology: The study was carried out with the sample size of 30 histopathologically diagnosed cases of oral squamous cell carcinoma and comparison of infiltration of these (tissue eosinophil and mast cells) inflammatory cells with control (normal) group of patients, was done by using special stains. Special stains are wonderful because they allow us to see which we cannot see with routine Haematoxylin and eosin stain. Special stains were used to demonstrate tissue eosinophils and mast cells. Carbol Chromotrope and congo red were used for tissue eosinophil and for mast cells staining toluidine blue and thionin were used.

Comparative study using Papanicolaou stain and silver-stained nucleolar organizer region counts in exfoliative smear of oral mucosa in bidi smokers and nonsmokers

Aim: The aim of this study is to compare the proliferative activity of exfoliated cells in bidi smokers and nonsmokers oral mucosa. Materials and Methods: The oral mucosal exfoliate smears were prepared from 40 individuals (20 nonsmokers and 20 smokers) with the age group ranging from 25 to 70 years, in and around Akola (Maharashtra). The Papanicolaou (PAP) stain and silver-stained nucleolar organizer region (AgNOR) were used to prepare cytogenic smear to evaluate the presence of cytological alterations, suggestive of inflammation, dysplasia, keratinization, and proliferative activity of epithelial cells. The present study involves PAP Class I and Class II smears. The obtained data were tabulated and statistically analyzed using statistical software IBM SPSS IBM Corp., Statistics for Windows, Version 20.0. Armonk, NY, USA: IBM Corp., and using t-test. Results: There was a significant difference in mean number of AgNORs/nucleus between nonsmokers (0.947 ± 0.2533) and smokers (3.021 ± 0.2256). There were 90% inflammatory changes observed in smokers whereas nonsmokers showed only 75% changes. PAP Class II changes, i.e., significant proliferative activity, were found between smokers and nonsmokers mucosa. Conclusion: A significant difference of AgNORs/nucleus was found between nonsmokers and smokers.

Cancellous osteoma of maxilla: A rare case report

Osteoma is a slow growing benign tumor result from either cancellous or compact bone proliferation. Osteoma of the jaws may arise from the surface of the bone as a sessile mass located either peripherally or endosteally. This paper presents a rare case report of peripheral cancellous osteoma, involving the buccal cortical plate and alveolar process of the right maxilla of a 37-year-old female patient.

Multidisciplinary Approach for an Initially Misdiagnosed Case of Osteosarcoma in a Medically Compromised Patient

Osteosarcoma is a highly malignant bone tumour. It is derived from primitive mesenchymal bone forming cell. Osteosarcoma of the jaw is quite rare. Despite its rarity, the dentist may be the first health professional who observes tumours involving the jaws. As there is a greater variability in clinical, radiological and histopathological findings of jaw osteosarcoma, so there is greater scope for multidisciplinary approach. An early definite diagnosis is must for successful treatment which can save patient’s life and esthetic. This article presents a case of osteosarcoma in medically compromised 17 years old female patient who was misdiagnosed initially and later on present as a huge size of the tumour mass with gross disfigurement than she was treated by multidisciplinary approach of oncosurgeon, medical oncologist and radiation oncologist with appropriate expertise.