Purnomo Soeharso
University of Indonesia
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Featured researches published by Purnomo Soeharso.
Reproductive Biology and Endocrinology | 2013
Dwi A. Pujianto; Evelyn Loanda; Puji Sari; Yurnadi H Midoen; Purnomo Soeharso
BackgroundEpididymal sperm maturation occurs via interactions between sperm and proteins secreted by the epididymal epithelium. Although this is an important process, the genes that encode the involved proteins remain largely uncharacterized. Previous studies have demonstrated that the genes involved in sperm maturation are regulated by androgen. Spag11a is an epididymal gene that is influenced by androgen. However, little is known about the putative role of this gene in the sperm maturation process. The objective of this study was to characterize Spag11a in the mouse epididymis.MethodsIn silico analyses were performed to predict signal peptides and functional domains. Spag11a expression was measured by quantitative real-time RT-PCR. Western blots and immunocytochemistry were performed to determine protein expression.ResultsSPAG11A is a member of the beta defensin protein family and constitutes a secretory protein. Spag11a was expressed exclusively in the epididymis. Moreover, it exhibited region-specific expression in the caput, which is typical for genes that are involved in creating a suitable microenvironment for sperm maturation. Mouse Spag11a was regulated by androgen. A significant decrease of Spag11a expression was observed at third day following a gonadectomy (P < 0.001). Interestingly, testosterone replacement therapy was able to maintain the expression almost at the normal level, indicating a dependency on androgen. Besides androgen, testicular factors influenced Spag11a expression in a different way. This was revealed by efferent duct ligation in which Spag11a was transiently up-regulated at the third day following the ligation before returning to the normal level at day 5. Spag11a regional expression was also observed at protein level detected by western immunoblotting which revealed a clear band in the caput but not in other regions. The prediction that SPAG11A is a secretory protein was confirmed by immunocytochemical analyses indicating cell-specific expression mainly in the caput principal cells and detection of the protein in epididymal luminal fluid and spermatozoa.ConclusionsBased on the characteristics of Spag11a, it is likely that this gene has a specific role in epididymal sperm maturation. Further studies using functional assays are necessary to confirm this finding.
Molecular Genetics & Genomic Medicine | 2017
Y Ariani; Purnomo Soeharso; Damayanti Rusli Sjarif
Genetics and genomic medicine in Indonesia.
Medical Journal of Indonesia | 2004
Purnomo Soeharso; Arfianti Arfianti; Nukman Moeloek
Medical Journal of Indonesia | 2015
Silvia W. Lestari; Dwi A. Pujianto; Purnomo Soeharso; Evelyn Loanda
Seminar Nasional ke-22 Perhimpunan Biologi Indonesia | 2013
Roselina Panghiyangani; Purnomo Soeharso; Dwi Anita Suryandari
Medical Journal of Indonesia | 2011
Yurnadi Yurnadi; Purnomo Soeharso; Dwi Anita Suryandari; Nukman Moeloek; R. Susworo
Journal of the Indonesian Medical Association | 2011
Yurnadi Yurnadi; Dwi Anita Suryandari; Purnomo Soeharso; Nukman Moeloek; R. Susworo
Archive | 2010
Dwi Anita Suryandari; Purnomo Soeharso; Nukman Moeloek; R. Susworo
Medical Journal of Indonesia | 2005
Purnomo Soeharso
Medical Journal of Indonesia | 2004
Yuni Ahda; Jörg Gromoll; Purnomo Soeharso; Septelia Inawati Wanandi; Nukman Moeloek