Qi Ma

Effect of Fructo-Oligosaccharide, Isomalto-Oligosaccharide, Inulin and Xylo-Oligosaccharide on Survival of B. Bifidum during Freeze-Drying

Four different prebiotics including fructo-oligosaccharide isomalto-oligosaccharide, inulin and xylo-oligosaccharide were screened for their effects on survival of Bifidobacterium bifidum during freeze-drying. The additions of prebiotics (v/v) were all 4%, 8%, 12%, 16% and 20%. The results showed that prebiotics could act on Bifidobacterium Bifidum. The optimum concentration of fructo-oligosaccharides (FOS) in samples for surival of Bifidobacterium bifidum was 12% and for viable count was 20％. Addition of xylo-oligosaccharides (XOS) played a significant role in promoting cell viablity when it was in low level. The optimum concentration of isomalto-oligosaccharides (IMO) for surival of Bifidobacterium bifidum was 16% and for viable count was 12％, while inulin in samples for surival and viable cell of Bifidobacterium bifidum both was 4％.

Effect of the Extract of Fructus tribuli on Growth of Lactobacillus Acidophilus

Effect of the extract of Fructus tribuli (EFT) on growth of Lactobacillus acidophilus LA04, LA05, LA06 and LA09 was studied by measuring optical density at 600nm (OD600) and pH using MRS culture media as the control. The addition of EFT (v/v) was 1%, 2%, 3%, 4% and 5%. Results were as follows: addition of EFT could promote the growth of Lactobacillus acidophilus LA09, The optimum concentration of EFT in MRS media was 1% at incubation 24h, but addition of EFT on growth of Lactobacillus acidophilus LA04, LA05 and LA06 has no significant effect.

Optimization of the medium for Lactobacillus acidophilus by Plackett-Burman and steepest ascent experiment.

BACKGROUND Lactobacillus acidophilus not only improves the intestinal flora balance but also inhabits the growth of undesirable microorganisms in intestine, which is benefit to the health of humans and animals. Plackett-Burman and steepest ascent experiment are the rapid and concise ways of screening the main effective factors. This study is aimed to select the main influence factors and optimize the medium for Lactobacillus acidophilus by Plackett-Burman experiment and steepest ascent experiment. METHODS The ideal carbon source was screened among glucose, maltose, lactose and whey powder, and the ideal nitrogen source was screened among casein hydrolysate, peptone, yeast extract powder, fish meal, carbamide, ammonium sulfate and sodium nitrate by single factor experiment. Plackett-Burman and steepest ascent experiment were applied to screen the main effective factors of Lactobacillus acidophilus among peptone, beef extract, yeast extract powder, glucose, K2HPO4, C6H14O7N2, CH3COONa, MgSO4 and Tween-80. Result. The results indicated that glucose (p = 0.01510) as negative factor and K2HPO4 (p = 0.02017) as positive effect were the significant growth factors of Lactobacillus acidophilus, CH3COONa (p = 0.09273) as positive effect was an important factor, and the optimized medium was as follows: glucose - 21 g/L, K2HPO4 - 3.5 g/L, CH3COONa - 6.5 g/L, peptone - 10 g/L, beef extract - 8 g/L, yeast extract pow. RESULTS nd. Lactobacillus acidophilus not only improves the intestinal flora balance but also inhabits the growth of undesirable microorganisms in intestine, which is benefit to the health of humans and animals. Plackett-Burman and steepest ascent experiment are the rapid and concise ways of screening the main effective factors. This study is aimed to select the main influence factors and optimize the medium for Lactobacillus acidophilus by Plackett-Burman experiment and steepest ascent experiment. Material and methods. The ideal carbon source was screened among glucose, maltose, lactose and whey powder, and the ideal nitrogen source was screened among casein hydrolysate, peptone, yeast extract powder, fish meal, carbamide, ammonium sulfate and sodium nitrate by single factor experiment. Plackett-Burman and steepest ascent experiment were applied to screen the main effective factors of Lactobacillus acidophilus among peptone, beef extract, yeast extract powder, glucose, K2HPO4, C6H14O7N2, CH3COONa, MgSO4 and Tween-80. Result. The results indicated that glucose (p = 0.01510) as negative factor and K2HPO4 (p = 0.02017) as positive effect were the significant growth factors of Lactobacillus acidophilus, CH3COONa (p = 0.09273) as positive effect was an important factor, and the optimized medium was as follows: glucose - 21 g/L, K2HPO4 - 3.5 g/L, CH3COONa - 6.5 g/L, peptone - 10 g/L, beef extract - 8 g/L, yeast extract powder - 8 g/L, C6H14O7N2 - 2 g/L, MgSO4 - 0.2 g/L and Tween-80 - 1 mL/L when the maximum viable count could achieve 2.72·109 cfu/mL. CONCLUSIONS The experimental model is reliable and the experimental results are of good stability. Variance analysis is performed to determine the adequacy and significance of the linear model. Thus, Plackett-Burman and steepest ascent experiment improve the veracity of optimization the medium for Lactobacillus acidophilus compared with the previous research.

The Effect of Feeding Neutralizer on the Growth of Bifidobacterium Bifidum

Abstract In order to investigative the effect of different neutralizers and their feeding time on culture of Bifidobacterium bifidum BB01, and pH, OD and viable count of B. bifidumBB01 in the medium in different time were measured. The results indicated that the NaOH solution was the optimum neutralizer compared with the others, and feeding time of the neutralizer to B. bifidum BB01 was 13 hours after inoculation. Furthermore, the OD value and viable count reached maximum at 21h (OD value= 1.667) and 20h (viable count: (3.52±0.046) ×109 CFU/mL) after the NaOH solution was added to the medium, respectively. In addition, the maximum OD value implied that the logarithmic phase of B. bifidum BB01 was delayed compared with the control and the viable count were 29.26% larger than the control group. The result of the study provides a method and an important basis for improving the viable counts of B. bifidum BB01.

Effect of Four Materials Including Trehalose, Soluble Starch, Raffinose and Galactose on Survival of Lactobacillus acidophilus during Freeze-Drying

Effect of four materials including trehalose, soluble starch, raffinose and galactose on survival of Lactobacillus acidophilus was studied by counting viable cells before and after freeze-drying. The concentrations of five carbohydrates were all 3%, 6%, 9%, 12% and 15%. Results showed as follows: the optimal concentrations of trehalose, soluble starch, raffinose and galactose were 12%, 9%, 3% and 15%, the survival of Lactobacillus acidophilus was 42.62%, 56.50%, 45.05% and 42.69% and the viable cells of lyophilized powder were 0.40×1011cfu/g,0.45×1011cfu/g, 0.52×1011 and 1.09×1011cfu/g, respectively.

Effect of Four Materials Including Glycine, Arginine, Proline and Sodium Glutamate on Survival of Bifidobacterium bifidum during Freeze-Drying

Effect of four materials including glycine, arginine, proline and sodium glutamate on survival of Bifidobacterium bifidum during freeze-drying was studied by measuring the viable counts of before and after freeze-drying. The addition of glycine, arginine, proline and sodium glutamate (%, w/v) were all 1.0%, 2.0%, 3.0%，4.0% and 5.0%. Results were as follows: addition of glycine, arginine, proline and sodium glutamate into B. bifidum paste could significantly improve the survival rate of B. bifidum and the number of viable cells of lyophilized powder. The optimal concentrations of glycine, arginine, proline and sodium glutamate were 4.0%, 4.0%, 3.0% and 2.0%, the survival of B. bifidum was 87.49%, 84.28%, 67.50% and 69.01%, respectively.