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Dive into the research topics where Qian-Lin Hao is active.

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Featured researches published by Qian-Lin Hao.


Journal of Immunology | 2009

IL-7 Dependence in human B lymphopoiesis increases during progression of ontogeny from cord blood to bone marrow.

Yasmin Khan Parrish; Ineavely Baez; Terry-Ann Milford; Abigail Benitez; Nicholas R. Galloway; Jaqueline Willeman Rogerio; Eva Sahakian; Mercy Kagoda; Grace Huang; Qian-Lin Hao; Yazmar Sevilla; Lora Barsky; Ewa Zielinska; Mary Price; Nathan R. Wall; Sinisa Dovat; Kimberly J. Payne

IL-7 is critical for B cell production in adult mice; however, its role in human B lymphopoiesis is controversial. One challenge was the inability to differentiate human cord blood (CB) or adult bone marrow (BM) hematopoietic stem cells (HSCs) without murine stroma. Here, we examine the role of IL-7 in human B cell development using a novel, human-only model based on coculturing human HSCs on primary human BM stroma. In this model, IL-7 increases human B cell production by >60-fold from both CB and adult BM HSCs. IL-7-induced increases are dose-dependent and specific to CD19+ cells. STAT5 phosphorylation and expression of the Ki-67 proliferation Ag indicate that IL-7 acts directly on CD19+ cells to increase proliferation at the CD34+ and CD34− pro-B cell stages. Without IL-7, HSCs in CB, but not BM, give rise to a small but consistent population of CD19lo B lineage cells that express EBF (early B cell factor) and PAX-5 and respond to subsequent IL-7 stimulation. Flt3 ligand, but not thymic stromal-derived lymhopoietin (TSLP), was required for the IL-7-independent production of human B lineage cells. As compared with CB, adult BM shows a reduction of in vitro generative capacity that is progressively more profound in developmentally sequential populations, resulting in an ∼50-fold reduction in IL-7-dependent B lineage generative capacity. These data provide evidence that IL-7 is essential for human B cell production from adult BM and that IL-7-induced expansion of the pro-B compartment is increasingly critical for human B cell production during the progression of ontogeny.


Journal of Immunology | 2000

IL-3 Increases Production of B Lymphoid Progenitors from Human CD34+CD38− Cells

Qian-Lin Hao; Denise Petersen; Lora Barsky; David C. Bockstoce

The effect of IL-3 on the B lymphoid potential of human hemopoietic stem cells is controversial. Murine studies suggest that B cell differentiation from uncommitted progenitors is completely prevented after short-term exposure to IL-3. We studied B lymphopoiesis after IL-3 stimulation of uncommitted human CD34+CD38− cells, using the stromal cell line S17 to assay the B lymphoid potential of stimulated cells. In contrast to the murine studies, production of CD19+ B cells from human CD34+CD38− cells was significantly increased by a 3-day exposure to IL-3 (p < 0.001). IL-3, however, did not increase B lymphopoiesis from more mature progenitors (CD34+CD38+ cells) or from committed CD34−CD19+ B cells. B cell production was increased whether CD34+CD38− cells were stimulated with IL-3 during cocultivation on S17 stroma, on fibronectin, or in suspension. IL-3Rα expression was studied in CD34+ populations by RT-PCR and FACS. High IL-3Rα protein expression was largely restricted to myeloid progenitors. CD34+CD38− cells had low to undetectable levels of IL-3Rα by FACS. IL-3-responsive B lymphopoiesis was specifically found in CD34+ cells with low or undetectable IL-3Rα protein expression. IL-3 acted directly on progenitor cells; single cell analysis showed that short-term exposure of CD34+CD38− cells to IL-3 increased the subsequent cloning efficiency of B lymphoid and B lymphomyeloid progenitors. We conclude that short-term exposure to IL-3 significantly increases human B cell production by inducing proliferation and/or maintaining the survival of primitive human progenitors with B lymphoid potential.


Blood | 2008

Expansion of multipotent and lymphoid-committed human progenitors through intracellular dimerization of Mpl.

Hisham Abdel-Azim; Yuhua Zhu; Roger P. Hollis; Xingchao Wang; Shundi Ge; Qian-Lin Hao; Goar Smbatyan; Donald B. Kohn; Michael Rosol; Crooks Gm

Self-renewal capacity is rapidly lost during differentiation of hematopoietic stem cells to lineage-committed progenitors. We demonstrate here that regulated intracellular signaling through the cytokine receptor Mpl induces profound expansion of not only multipotent (ie, lymphomyeloid) but also lymphoid-committed human hematopoietic progenitors. A fusion protein containing the intracellular signaling domain of Mpl and a dimerization domain was constitutively expressed in populations enriched in human lymphomyeloid progenitor/stem cells (CD34(+)CD38(-)Lin(-)CD7(-)) and multilymphoid progenitors (CD34(+)CD38(-)Lin(-)CD7(+)). Intracellular dimerization of Mpl in target cells was induced by in vitro or in vivo administration of a diffusible synthetic ligand. In vitro, Mpl dimerization produced divisions of clonogenic, multilineage CD34(+) cells able to engraft immunodeficient mice. When dimerization was induced in vivo after transplantation of either lymphomyeloid or multilymphoid progenitors, donor-derived hematopoiesis was sustained for at least 12 weeks and primitive CD34(+)Lin(-) progenitors were expanded more than 1000-fold. Lineage potential of progenitors was not altered and differentiation was not prevented by synthetically induced Mpl signaling. These data demonstrate that dimerization of a single cytokine receptor can deliver a profound expansion signal in both uncommitted and lymphoid-committed human hematopoietic progenitors.


Blood | 1996

Extended long-term culture reveals a highly quiescent and primitive human hematopoietic progenitor population

Qian-Lin Hao; Ft Thiemann; D Petersen; Smogorzewska Em; Crooks Gm


Blood | 2001

Identification of a novel, human multilymphoid progenitor in cord blood

Qian-Lin Hao; Judy Zhu; Mary Price; Kimberly J. Payne; Lora Barsky


Blood | 1998

In vitro identification of single CD34+CD38- cells with both lymphoid and myeloid potential.

Qian-Lin Hao; Smogorzewska Em; Lora Barsky; Crooks Gm


Blood | 2007

Human intrathymic lineage commitment is marked by differential CD7 expression: identification of CD7− lympho-myeloid thymic progenitors

Qian-Lin Hao; Ann George; Judy Zhu; Lora Barsky; Ewa Zielinska; Xingchao Wang; Price M; Ge S; Crooks Gm


Human Gene Therapy | 1995

Expression of Biologically Active Human Factor IX in Human Hematopoietic Cells after Retroviral Vector-Mediated Gene Transduction

Qian-Lin Hao; Punam Malik; Roberto Salazar; Hui Tang; Erlinda M. Gordon; Donald B. Kohn


Clinical Immunology | 2007

An All-Human Culture Model Supporting Human B Lymphopoesis

Mercy Kagoda; Yasmin Khan Parrish; Jaqueline Willeman Rogerio; Ineavely Baez; Qian-Lin Hao; Lora Barsky; Ewa Zielinska; Monika Smogorzewska; Kimberly J. Payne


Clinical Immunology | 2006

Sa.48. IL-7-Induced Expansion of the Human Pre-B-Cell Compartment in Nonfetal B-Cell Development

Yasmin K. Parrish; Jaqueline Rogerio; Eva Sahakian; Grace Huang; Qian-Lin Hao; Lora Barsky; Ewa Zielinska; Price M; Kimberly J. Payne

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Lora Barsky

Children's Hospital Los Angeles

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Ewa Zielinska

Children's Hospital Los Angeles

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Crooks Gm

Children's Hospital Los Angeles

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Kimberly J. Payne

Children's Hospital Los Angeles

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Yuhua Zhu

Children's Hospital Los Angeles

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Donald B. Kohn

University of California

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Price M

Children's Hospital Los Angeles

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Ann George

Children's Hospital Los Angeles

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Eva Sahakian

Children's Hospital Los Angeles

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Grace Huang

Children's Hospital Los Angeles

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