Qingchuan Zhang

Fourier-based interpolation bias prediction in digital image correlation.

Based on the Fourier method, this paper deduces analytic formulae for interpolation bias in digital image correlation, explains the well-known sinusoidal-shaped curves of interpolation bias, and introduces the concept of interpolation bias kernel, which characterizes the frequency response of the interpolation bias and thus provides a measure of the subset matching quality of the interpolation algorithm. The interpolation bias kernel attributes the interpolation bias to aliasing effect of interpolation and indicates that high-frequency components are the major source of interpolation bias. Based on our theoretical results, a simple and effective interpolation bias prediction approach, which exploits the speckle spectrum and the interpolation transfer function, is proposed. Significant acceleration is attained, the effect of subset size is analyzed, and both numerical simulations and experimental results are found to agree with theoretical predictions. During the experiment, a novel experimental translation technique was developed that implements subpixel translation of a captured image through integer pixel translation on a computer screen. Owing to this remarkable technique, the influences of mechanical error and out-of-plane motion are eliminated, and complete interpolation bias curves as accurate as 0.01 pixel are attained by subpixel translation experiments.

Development of Protein A Functionalized Microcantilever Immunosensors for the Analyses of Small Molecules at Parts per Trillion Levels

Development of microcantilever biosensors for small molecules was exemplified with the beta-adrenergic agonist clenbuterol and the antibiotic chloramphenicol. In this paper, antibody sulfhydrylation and protein A were used to modify the microcantilever Au surface, and the antibody activities on the microcantilever were evaluated with direct competitive enzyme-linked immunosorbent assay (dcELISA). The activity of the antibodies immobilized on the microcantilever via protein A was 1.7-fold of that via the sulfhydrylation reagent 2-iminothiolane hydrochloride. A microcantilever immunosensor method with protein A as the functionalization reagent was established to detect the residues of clenbuterol and chloramphenicol at limits of detection (LOD) of approximately 0.1 and 0.2 ng/mL, respectively. Such LODs were better than that of the corresponding dcELISAs. The concentration of clenbuterol in a fortified feed sample detected with the microcantilever immunosensor after thorough extraction and purification agreed well with that detected with the dcELISA. Protein A showed to be simple and reproducible for functionalization of the antibodies on the Au surface and, thus, has common application values in microcantilever immunosensor development. The results suggest that microcantilever immunosensors be suitable for detection of small molecules, and the assay sensitivity is mainly related to the quality and activities of the antibodies.

Dynamic observation of localized strain pulsation generated in the plastic deformation process by electronic speckle pattern interferometry

An electronic speckle pattern interferometry (ESPI) system, which makes it possible to observe dynamic phenomena, was applied to investigate the plastic deformation process in tensile experiments of aluminum alloy samples. The dynamic behavior of a strain-localized band which propagated through a specimen was thoroughly investigated. A pulsating fringe variation of the order of 1 Hz was discovered to be the fine structure of the propagating band. Fringe pulsation is caused by localized strain pulsation which suggests that the stress relaxation process propagates periodically with a time constant which governs the dissipative characteristics of a heterogeneous material.

A novel uncooled substrate-free optical-readable infrared detector: design, fabrication and performance

A novel substrate-free uncooled IR detector based on an optical-readable method is presented and fabricated successfully. The detector is composed of a bi-material (BM) cantilever array, without a silicon substrate, which is eliminated in the fabrication process. Compared with the generally used sacrificial layer cantilever, the loss of incident IR energy caused by the reflection from and absorption by the silicon substrate is eliminated completely in the substrate-free structure. The IR radiation reaching the IR detector surface increases by over 80% in the case of the novel substrate-free detector array structure, compared to the sacrificial layer structure. Moreover, the substrate-free structure has less heat loss than the sacrificial layer structure. The results of thermal imaging of the human body show the detector is able to sense objects at room temperature. The experimental NETD was estimated to be 200 mK.

Noise-induced bias for convolution-based interpolation in digital image correlation.

In digital image correlation (DIC), the noise-induced bias is significant if the noise level is high or the contrast of the image is low. However, existing methods for the estimation of the noise-induced bias are merely applicable to traditional interpolation methods such as linear and cubic interpolation, but are not applicable to generalized interpolation methods such as BSpline and OMOMS. Both traditional interpolation and generalized interpolation belong to convolution-based interpolation. Considering the widely use of generalized interpolation, this paper presents a theoretical analysis of noise-induced bias for convolution-based interpolation. A sinusoidal approximate formula for noise-induced bias is derived; this formula motivates an estimating strategy which is with speed, ease, and accuracy; furthermore, based on this formula, the mechanism of sophisticated interpolation methods generally reducing noise-induced bias is revealed. The validity of the theoretical analysis is established by both numerical simulations and actual subpixel translation experiment. Compared to existing methods, formulae provided by this paper are simpler, briefer, and more general. In addition, a more intuitionistic explanation of the cause of noise-induced bias is provided by quantitatively characterized the position-dependence of noise variability in the spatial domain.

Design, Fabrication, and Characterization of a 240

We report a significant step in the design, fabrication, and performance evaluation of a 240 × 240 microelectromechanical system uncooled infrared (IR) focal plane array (FPA) with 42-μm pitch pixels. An improved analytical model has been developed to optimize the design. The optimal key parameters have been verified through experiments, including thermal transmission efficiency, thermomechanical sensitivity, thermal sensitivity, and response time. Compared with our previous work, the number of the fabricated FPAs pixels is increased by 125% and the corresponding pixels area is decreased by 51%. Furthermore, our FPA has a good sensitivity with a noise equivalent temperature difference of about 373 mK, thus providing an extension of state-of-the-art IR FPA and practical information for future applications.

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Generation of microcantilever bending from biochemical interactions can have wide applications, ranging from high-throughput molecular detection to bioactuation. However, the origin of the biochemically induced surface stress causing the bending is a subject of much scientific debate and interest. Unlike a compressive surface stress caused by biomacromolecule antigen and antibody binding, here we show that a small molecule antigen and antibody binding on the surface gives rise to a tensile stress. We propose that the tensile stress is induced by antibody conformational change which manifests itself as Fab arm motion that exposes the C1q binding site of the antibody due to antigen binding. A microcantilever immunosensor was developed for the detection of Chlorimuron-ethyl (CE). We found that antibodies with oriented immobilization induce a greater resultant surface stress than those with random immobilization. The length of linker between the surface and the antibody plays an important role on the stress transmission. The shorter the length, the greater the surface stress. These mechanism and principles will underpin the design of devices and coatings to significantly lower the small molecule detection limit and may also have an impact on our understanding of antigen and antibody binding.

240 MEMS Uncooled Infrared Focal Plane Array With 42-

We demonstrate the photophoretic trapping of more than several hundreds of absorbing particles by tapered-ring optical traps diffracted from a circular aperture. The experiments with different laser powers show the influence of air flow acting on particles. Three kinds of particles with different densities (about 1~7 g/cm(3)) and different shapes (spherical, non-spherical) can be trapped. The non-spherical particles (toner particles) disperse in optical field, while the spherical particles (ink droplets and iron particles) arrange as a straight line. More importantly, in the experiments of two counter-propagating tapered-ring beams, the agglomeration of particles is achieved and can help research the dynamics of aerosols.

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Pixelated micropolarizer array (PMA) is a novel concept for real-time visible imaging polarimetry. A 320 × 240 aluminum PMA fabricated by electron beam lithography is described in this paper. The period, duty ratio, and depth of the grating are 140 nm, 0.5, and 100 nm, respectively. The units are standard square structures and the metal nanowires of the grating are collimating and uniformly thick. The extinction ratio of 75 and the maximum polarization transmittance of 78.8% demonstrate that the PMA is suitable for polarization imaging. When the PMA is applied to real-time polarization imaging, the degree of linear polarization image and the angle of linear polarization image are calculated from a single frame image. The polarized target object is highlighted from the unpolarized background, and the surface contour of the target object can be reflected by the polarization angle.

Pitch Pixels

The improvement of sensitivity is of great significance to the application of biochemical sensor. In this study, we propose a micocantilever-based immunosensor in surface stress mode using half antibody fragments as receptor molecules. The thiol-containing half antibody fragment was obtained with a low loss of antibody biological activity and then was covalently and orientedly immobilized on the gold surface of microcantilevers via two native thiol groups. Such a one-step reaction and immobilization of receptor molecule simplify the preparation process of micocantilever immunosensor. Using shortened and highly oriented half antibody fragments as receptor molecules, the generation of surface stress and the transmission of stress from the interaction region of molecules to the surface of the microcantilever have been elevated significantly. The limit of detection (LOD) of the presented sensor has been significantly lowered to 1 pg/mL, or 1.1 pM in equivalence, which is a 500-fold improvement when compared with intact full antibody coated conventional micocantilever sensors. The results indicate that the half antibody fragment is well suited for the functionalization of the microcantilever surface and is generally applicable to all microcantilever immunosensor development, and this principle will help to design a functional film of devices with significantly lower LOD.