Qiulai He

Microbial population dynamics during sludge granulation in an A/O/A sequencing batch reactor.

The evolution of the bacterial population during formation of denitrifying phosphorus removal granular sludge was investigated using high-throughput pyrosequencing. As a result, mature granules with a compact structure were obtained in an anaerobic/aerobic/anoxic (A/O/A) sequencing batch reactor under an organic loading rate as low as 0.3kg COD/(m(3)·d). Rod-shaped microbes were observed to cover with the outer surface of granules. Besides, reliable COD and simultaneous nitrogen and phosphorus removal efficiencies were achieved over the whole operation period. MiSeq pyrosequencing analysis illustrated that both the microbial diversity and richness increased sharply during the granulation process, whereas they stayed stable after the presence of granules. Some microorganisms seemed to contribute to the formation of granules, and some were identified as functional bacterial groups responsible for constructing the biological reactor.

Unraveling characteristics of simultaneous nitrification, denitrification and phosphorus removal (SNDPR) in an aerobic granular sequencing batch reactor.

An aerobic granular sequencing batch reactor (SBR) on an aerobic/oxic/anoxic (AOA) mode was operated for 50days with acetate sodium as the sole carbon source for simultaneous carbon, nitrogen and phosphorus removal. Excellent removal efficiencies for chemical oxygen demand (COD) (94.46±3.59%), nitrogen (96.56±3.44% for ammonia nitrogen (NH4(+)-N) and 93.88±6.78% for total inorganic nitrogen (TIN)) and phosphorus (97.71±3.63%) were obtained over operation. Mechanisms for simultaneous nutrients removal were explored and the results indicated that simultaneous nitrification, denitrification and phosphorus removal (SNDPR) under aerobic conditions was mainly responsible for most of nitrogen and phosphorus removal. Identification and quantification of the granular AOA SBR revealed that higher rates of nutrients removal and more potentials were to be exploited by optimizing the operating conditions including time durations for AOA mode and the feeding compositions.

Chronic responses of aerobic granules to zinc oxide nanoparticles in a sequencing batch reactor performing simultaneous nitrification, denitrification and phosphorus removal

The reactor performance, granules characteristics and microbial population dynamics were investigated to assess the chronic responses of aerobic granules to zinc oxide nanoparticles (ZnO NPs) of 0, 5, 10 and 20mg/L for a period of 180days. The results showed that ZnO NPs stimulated COD removal, whereas caused inhibition to both nitrification and denitrification. However, biological phosphorus removal remained effective and stable. Introduction of ZnO NPs sharply decreased the respiration of granules, while did not change the settleability. Both content of extracellular polymeric substances (EPS) and the ratio of protein to polysaccharides (PN/PS) rose significantly. MiSeq pyrosequencing was employed to explore the microbial population dynamics. Results demonstrated that up to 20mg/L reduced the alpha-diversity of bacterial communities. Finally, phylogenetic classification of the dominant functional species involved in biological nutrients removal were identified to assess the effects of ZnO NPs to aerobic granules from the molecular level.

Performance and microbial population dynamics during stable operation and reactivation after extended idle conditions in an aerobic granular sequencing batch reactor

The evolution of removal performance and bacterial population dynamics of an aerobic granular sequencing batch reactor were investigated during stable operation and reactivation after prolonged storage. The system was run for a period of 130days including the stable condition phase, storage period and the subsequent reactivation process. Excellent removal performance was obtained during the stable operation period, which was decayed by the extended idle conditions. The removal efficiencies for both carbon and nitrogen decayed while phosphorus removal remained unaffected. Both granules structure and physical properties could be fully restored. Microbial populations shifted sharply and the storage perturbations irreversibly altered the microbial communities at different levels. Extracellular polymeric substances (especially protein) and key groups were identified as contributors for storage and re-startup of the aerobic granular system.

Studies of malachite green adsorption on covalently functionalized Fe3O4@SiO2–graphene oxides core–shell magnetic microspheres

This paper presents the synthesis of a novel core–shell covalently functionalized Fe3O4 coated SiO2 decorated graphene oxides (Fe3O4@SiO2–GO) adsorbent. The prepared Fe3O4@SiO2–GO was characterized by fourier transform infrared spectrum, X-ray diffraction(XRD), X-ray photoelectron spectroscopy (XPS), and magnetic measurements. And the adsorption properties of the Fe3O4@SiO2–GO toward malachite green were systematically investigated. Various factors possibly affecting the adsorption behavior (initial concentration, contact time, and adsorbent dosage) were also studied in detail. The adsorption capacity (qm) of Fe3O4@SiO2–GO for malachite green was found to be 265.87 mg/g with best fit to Langmuir isotherm (R2 = 0.968), and the adsorption mechanism follows the pseudo-second-order model (R2 = 0.981). The results indicate that the Fe3O4@SiO2–GO adsorbent, which is easily separated via an external magnetic field, is a potential low-cost effective material for malachite green removal from contaminated water.Graphical Abstract

Elucidation of microbial characterization of aerobic granules in a sequencing batch reactor performing simultaneous nitrification, denitrification and phosphorus removal at varying carbon to phosphorus ratios

An aerobic granules simultaneous nitrification, denitrification and phosphorus removal (SNDPR) system was evaluated in terms of the reactor performance and microbial population dynamics with decreasing C/P ratios from 50 to 16. The effects of C/P ratios on organic carbon and nutrients removal were investigated, as well as the alpha diversity of the bacterial community and bacterial compositions by using Illumina MiSeq pyrosequencing technology. Finally, the relative abundances and distribution patterns were identified and assessed given the key functional groups involved in biological nutrients removals to reveal the effects of C/P ratios to aerobic granules in the SNDPR from the molecular level.

Characteristics of Nitrate Reduction Using Fe (II) as Electron Donor in Activated Sludge

ABSTRACT Static experiments were conducted to investigate the effects of environmental factors on nitrate (NO3−-N)-removal efficiency, such as NO3−-N loading, pH value, C/N ratio and temperature in activated sludge using Fe (II) as electron donor. The results demonstrated that the average denitrification rate increased from 1.25 to 2.23 mg NO3−-N/(L·h) with NO3−-N loading increased from 30 to 60 mg/L. When pH increased from 7 to 8, the concentration of NO3−-N and nitrite (NO2−-N) in effluent were all maintained at quite low levels. C/N ratio had little impact on denitrification process, i.e., inorganic carbon (C) source could still be enough for denitrification process with C/N ratio as low as 5. Temperature had a significant effect on the denitrification efficiency, and NO3−-N removal efficiency of 92.03%, 96.77%, 97.67% and 98.23% could be obtained with temperature of 25°C, 30°C, 35°C and 40°C, respectively. SEM, XRD and XRF analysis was used to investigate microscopic surface morphology and chemical composition of the denitrifying activated sludge, and mechanism of the nitrate-dependent anaerobic ferrous oxidation (NAFO) bacterias could be explored with this research.

Hybrid functionalized chitosan-Al 2 O 3 @SiO 2 composite for enhanced Cr(VI) adsorption

In this study, we prepared a novel hybrid functionalized chitosan-Al2O3@SiO2 composite (FCAS) for removing hexavalent chromium [Cr(VI)] from aqueous system. Spectroscopic studies like Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), field emission scanning electron microscope (FESEM) and energy dispersive spectroscope (EDS) were characterized. The effects such as dosage of adsorbent, pH, contact time and initial Cr(VI) concentration were evaluated. It has been illustrated that a wide acidic condition in the pH range of 2-6 was conducive to Cr(VI) adsorption and only 10 min was required to reach about 80% adsorption. Also, the adsorption properties of prepared adsorbent such as kinetics, thermodynamics and isotherms were comprehensively studied. Additionally, the adsorption capacity barely declined even after five cycles. Studies found that FCAS with characteristics of high performance of adsorption rate and capacity and better reusability would be a potential adsorbent for wastewater treatment.

Simultaneous nitrification, denitrification and phosphorus removal in aerobic granular sequencing batch reactors with high aeration intensity: Impact of aeration time

A new operating approach by reducing the aeration time while keeping high intensity was evaluated for enhanced nutrients removal and maintenance of granular stability. Three aerobic granular sequencing batch reactors (SBR) performing simultaneous nitrification, denitrification and phosphorus removal (SNDPR) were run at different aeration time (120, 90, and 60 min). Aerobic granules could remain their integrity and stability over long-term operation under high aeration intensity and different time, and shorter aeration time favored the retention of biomass, better settleability, and more production of extracellular polymeric substances (EPS). Besides, efficient and stable reactor performance for carbon and phosphorus were achieved, especially, enhanced nitrogen removal was obtained due to reduction of aeration time. Further exploration revealed that the aeration time shaped the bacterial community in terms of diversity, composition, as well as the distribution of functional groups involving carbon, nitrogen and phosphorus removal.

Simultaneous nitrification, denitrification and phosphorus removal in an aerobic granular sludge sequencing batch reactor with high dissolved oxygen: Effects of carbon to nitrogen ratios

Simultaneous nitrification, denitrification and phosphorus removal (SNDPR) using aerobic granules is a promising approach in water treatment. The present work investigated the effects of influent carbon to nitrogen (N) ratios (20, 10, and 4) on the SNDPR performance in aerobic granular sequencing batch reactors (AGSBR) under high aeration rate. Results revealed that granules remained long-term stability when the DO concentration was 7-8 mg/L. With the decline of COD/N ratios, the denitrification efficiency was reduced due to the accumulation of nitrate, although the removal of COD and TP remained stable with good efficiency. Rising concentration of ammonia N led to the increase of PN/PS ratio of EPS as well as the protein types according to the results of 3D-EEM fluorescence spectroscopy. MiSeq pyrosequencing technology indicated that the decreasing ratio of COD/N under high DO concentration contributed to accumulation of GAOs and DNPAOs rather than PAOs.