R. A. Binaghi

Effect of heating at 56°C on mouse IgE antibodies

Abstract The thermolability at 56°C of mouse IgE antibodies, measured by their PCA activity, was studied in various experimental conditions. Mouse IgE antibodies are partially protected against heat inactivation at 56°C when the sera containing them are previously diluted in saline or phosphate media. The protection is dependant upon the dilution and the molarity of the medium, and upon the pH: it is best at higher molarity and at pH 7. The rate of heat inactivation depends upon the concentration of serum proteins in the diluting medium; it is increased by the presence of immunoglobulins and not of albumins. It is also increased in the presence of reducing, alkylating or denaturating agents. These results indicate that heat inactivation of mouse IgE antibodies dependes upon experimental conditions and that ceratin precuations should be taken in using thermolability as a criterion to distinguish IgE from other classes of anaphylactic antibodies.

Antibody-dependent mechanisms of immunity against migrating larvae of Trichinella spiralis

Abstract Normal rat peritoneal exudate cells adhere to new-born Trichinella spiralis larvae in the presence of specific antibodies produced during the course of an oral infection. After 24 hours incubation with the peritoneal cells and antibodies most of the larvae were killed. Heated immune serum had less activity. With normal serum no adherence was observed. In order to investigate the influence of antibodies on new-born larvae in vivo, larvae were pre-treated in vitro with immune serum and then inoculated intravenously into normal mice. After 30 days the yield of normal larvae was 50% of the number recovered in a control group infected with non-treated new-born larvae.

Production of different classes of immunoglobulins in rats infested with trichinella spiralis

Abstract Immunoglobulins IgGa, IgGb, IgGc, IgR (or IgG 1 ), IgM and IgE were analyzed in the serum of rats of three different strains, normal or heavily infested with Trichinella spiralis . The analysis were performed by quantitative immunochemical methods employing specific antisera against each class of immunoglubulin. A very important increase in the levels of IgE and IgR was observed in the infested animals. In regard to the other immunoglobulins, only IgM appears to be increased in a moderate but significant proportion.

Rapid and high production of precipitating antibodies in rats

Abstract Rats were inoculated into the foot-pads with 0.2–1 mg antigen mixed with complete Freunds adjuvant or Bordetella pertussis . When DNP-protein conjugates or heterologous immunoglobulins were used as antigen, serum concentrations of 1–2 mg antibody per ml were usually obtained after 11 days. After a booster injection, made in the same way, a few weeks later, much higher titres were frequently observed with most antigens. With microgram doses of antigen, good precipitating antibody production was observed only after booster injections.

Heterogeneity of Guinea Pig γ1and γ2 Immunoglobulins

Heterogeneity was shown by immunoelectrophoresis in the Fc fragments of normal and anti-DNP guinea pig γ1and γ2immunoglobulins, suggesting the existence of subclasses. An attempt