R. Gerritsen

Association of leptin gene polymorphisms with serum leptin concentration in dairy cows

Leptin is a hormone produced by adipocytes, and its expression is regulated by body fatness and energy balance. This study describes the association of four leptin gene polymorphisms in dairy cows (R4C, A59V, RFLP1, and BM1500) with circulating leptin concentrations during the periparturient period. A59V is located at a between-species conserved region of leptin, and R4C might have effect on the tertiary structure of the leptin protein because of the presence of an extra cystein. RFLP1 is an intronic SNP and BM1500 is a microsatellite located 3.6 kb downstream of the leptin locus. The four polymorphisms were genotyped in 323 HF heifers with known pedigree. Leptin concentrations were determined biweekly from 30 days before until 80 days after parturition. The effect of genotype on leptin concentrations was modeled by fitting a spline in ASREML describing leptin concentrations as a function of days relative to parturition for each genotype/allele. Surprisingly, associations were found during pregnancy, but not during lactation. This indicates that the polymorphism could be more effective during pregnancy. If further studies demonstrate that more leptin-binding protein (Ob-Re) is present in this stage, it is hypothesized that a structural difference in the leptin protein could cause a sub-optimal binding stringency to Ob-Re. Free leptin could be cleared faster than bound leptin, and this could result in lower leptin concentrations during pregnancy for the polymorphism. The effects found might be ascribed to R4C. However, more study on the Ob-Re receptor, like binding stringencies between R4C and wild-type leptin and glycosylation during pregnancy, would provide more insight in the results found.

Peri‐oestrus Hormone Profiles and Follicle Growth in Lactating Sows with Oestrus Induced by Intermittent Suckling

This study describes follicle dynamics, endocrine profiles in multiparous sows with lactational oestrus compared with conventionally weaned sows (C). Lactational oestrus was induced by Intermittent Suckling (IS) with separation of sows and piglets for either 12 consecutive hours per day (IS12, n = 14) or twice per day for 6 h per occasion (IS6, n = 13) from day 14 of lactation onwards. Control sows (n = 23) were weaned at day 21 of lactation. Pre-ovulatory follicles (> or =6 mm) were observed in 100% of IS12, 92% of IS6 and 26% of C sows before day 21 of lactation and in the remaining 74% C sows within 7 days after weaning. All sows with pre-ovulatory follicles showed oestrus, but not all sows showed ovulation. Four IS6 sows and one IS12 sow developed cystic follicles of which two IS6 sows partially ovulated. Follicle growth, ovulation rate and time of ovulation were similar. E(2) levels tended to be higher in IS sows (p = 0.06), the pre-ovulatory LH surge tended to be lower in IS12 (5.1 +/- 1.7 ng/ml) than in C sows (8.4 +/- 5.0 ng/ml; p = 0.08) and P(4) levels were lower in IS12 and IS6 than in C sows (at 75 h after ovulation: 8.8 +/- 2.4 ng/ml vs 7.0 +/- 1.4 ng/ml vs 17.1 +/- 4.4 ng/ml; p < 0.01). In conclusion, sows with lactational oestrus induced by IS are similar to weaned sows in the timing of oestrus, early follicle development and ovulation rates, but the pre-ovulatory LH surge and post-ovulatory P(4) increase are lower.

The Intermittent Suckling Regimen in Pigs: Consequences for Reproductive Performance of Sows

Intermittent suckling (IS), a system in which the sow and the piglets are separated for a number of hours per day during lactation, is put forward as a system that can increase piglet welfare around weaning and also induce lactational oestrus. To be of practical use, IS regimes need to result in a predictable oestrus and good reproductive performance. This review describes the effects of IS on sow reproductive performance. During IS, the LH pulsatility pattern switches to a high frequency, low amplitude release during separation, as is normally observed at weaning. In sows that ovulate during IS, LH pulsatility remains higher than in anovulatory sows. Around 90% of the IS sows show follicle growth up to pre-ovulatory size in a similar time period as sows post-weaning. Depending on the stage of lactation that IS commenced, 13% (day 21) to 21% (day 14) of the sows with pre-ovulatory follicles did not ovulate, but follicles either regressed or developed into cystic follicles. In the ovulatory IS sows oestradiol production is comparable but the LH surge is lower with an early start of IS (day 14) when compared with the weaned sows. Continuation of IS post-ovulation has negative effects on pregnancy rate, embryo development and progesterone levels. Factors related to lactation (e.g. a high metabolic clearance rate), may play a role in this. In conclusion, IS can result in a fertile oestrus when the timing of start of IS and duration of IS are taken into account, but for practical use IS regimes result in a too variable reproductive performance.

Timing of lactational oestrus in intermittent suckling regimes: consequences for sow fertility.

Three intermittent suckling (IS) regimes were evaluated for their effects on lactational oestrus and subsequent fertility. Control sows were weaned (CW; n = 38) at d 26 ± 2 of lactation. In IS19-7D (n=40) and IS19-14D (n=42) sows, IS started at d 19 ± 1 of lactation and sows were weaned 7 or 14 d later. In IS26-7D (n=41), IS started at d 26 ± 1 of lactation and sows were weaned 7d later. During IS, sows were separated from their piglets for 10h/day. Oestrus detection was performed twice daily without a boar and ovulation was confirmed by ultrasound once a week. In IS19-7D, IS19-14D and IS26-7D, respectively, 50%, 64% and 61% of the sows showed oestrus and ovulation during IS (P>0.05), and, of the remaining sows, 100%, 93%, and 69% showed oestrus in the first week after weaning. In CW sows, 95% showed oestrus in the first week after weaning. Parity 1 sows were considerably less likely than older parities (23% vs. 68%) to show oestrus in lactation. Pregnancy rate of the first post partum oestrus (during lactation or after weaning) was 89% (CW), 92% (IS19-7D), 80% (IS19-14D) and 77% (IS26-7D) (P>0.05) and subsequent litter size was 14.5 ± 0.5, 14.5 ± 0.6, 15.3 ± 0.5 and 15.2 ± 0.8, respectively (P>0.05). Sows mated during lactation had similar pregnancy rate and litter size to those mated after weaning. Hence, ongoing lactation for the first 2-9 d of pregnancy did not negatively affect fertility. A total of 50-64% of IS sows showed lactational oestrus, regardless of the stage of lactation. Pregnancy rates and litter size were similar to control sows, and were not affected by stage of lactation at mating.

Intermittent suckling enables estrus and pregnancy during lactation in sows: Effects of stage of lactation and lactation during early pregnancy

Previously we demonstrated that pre-ovulatory LH and post-ovulatory progesterone (P4) concentrations in plasma were low and embryo development was retarded when sows were induced to ovulate during lactation by submitting them to intermittent suckling (IS). The present study investigated whether this was due to: (1) stage of lactation when IS was initiated, and (2) continuation of IS post-ovulation. Multiparous Topigs40 sows were studied under three conditions: conventional weaning at Day 21 of lactation (C21; n=30), intermittent suckling from Day 14 of lactation (IS14; n=32), and intermittent suckling from Day 21 of lactation (IS21; n=33). Sows were separated from piglets for 12h daily during IS. IS sows were either weaned at ovulation or 20d following ovulation. One-third (21/63) of the IS21 and C21 sows had already ovulated or had large pre-ovulatory follicles at Day 21 and were excluded from further study. Initiation of IS at Day 14 instead of Day 21 of lactation tended to reduce P4 at 7d post-ovulation (P=0.07), did not affect pregnancy rate, and tended to reduce embryo survival (P=0.06). Continuation of IS during pregnancy resulted in lower P4 at 7 and 12d post-ovulation, tended to reduce embryo weight and pregnancy rate (P<0.10), whereas embryo survival was not affected. This study presents data for a population of sows in which follicle growth and ovulation are easily triggered under suckling conditions. Further, when these sows are bred during lactation, initiation of IS at 21 rather than 14d of lactation with weaning at ovulation yields the most desirable reproductive performance.

Porcine Luteal Function in Relation to IGF-1 Levels Following Ovulation During Lactation or After Weaning

This study presents relationships between peripheral progesterone and Insulin-like Growth Factor-1 (IGF-1) concentrations during the early luteal phase in sows. Data were derived from three experiments, one with primiparous weaned sows (n = 21) and two with multiparous sows that either ovulated during lactation (n = 23) or after weaning (n = 12). The sows that ovulated during lactation did so due to an intermittent suckling regime (inhibition of suckling for 12 h each day from day 14 of lactation) or due to treatment with PG600. IGF-1 concentrations varied considerably among experiments, and were the lowest in the multiparous sows, regardless of whether they were weaned or lactating: 68 +/- 5 and 85 +/- 8 ng/ml in the two experiments with multiparous sows vs 188 +/- 15 ng/ml in the primiparous sows. Progesterone concentrations were lowest for the lactating sows. Overall, the increase in progesterone during the early luteal phase was strongly correlated with IGF-1 concentrations (r = 0.7). However, the correlation was low in multiparous lactating sows (r = 0.28; p < 0.10) and nonsignificant in multiparous weaned sows (r = 0). The weaned multiparous sows had IGF-1 levels comparable to lactating multiparous sows, but higher progesterone levels. In conclusion, these data show a positive relationship between peripheral IGF-1 and progesterone concentrations in vivo during the early luteal phase. In lactating sows, IGF-1 concentrations are probably a limiting factor for progesterone secretion, although other factors may be involved.

Early embryo survival and development in sows with lactational ovulation.

During lactation, daily separation of sow and piglets, intermittent suckling (IS), can induce lactational oestrus and ovulation. This study examined effects of IS on subsequent early embryo survival and development. Multiparous Topigs40 sows were separated from their piglets for either 12 consecutive hours per day (IS12, n = 13) or two times for 6 h per day (IS6, n = 10) from day 14 of lactation onwards until 23 days after ovulation. Control sows (C, n = 17) were weaned at day 21 of lactation. Oestrus was shown in all treatments within 5 days after the start of treatment. Sows were inseminated each day of oestrus and slaughtered at D23 after ovulation. Intermittent suckling did not significantly affect pregnancy rates of sows (75% IS12 vs 78% IS6 vs 94% C; p > 0.10). Embryo survival was not significantly affected by IS (IS12: 57%; IS6: 51%; p > 0.10) although it seemed to be lower than in C sows (70%). Some parameters of embryo, placental and uterine development were affected by IS, especially in the IS6 group. IS6 embryos had shorter placentas (17.5 +/- 1.2 cm; p < 0.05) than C (20.3 +/- 1.4 cm) and IS12 sows (20.9 +/- 0.7 cm) were smaller and less developed than C sows (p < 0.05). In conclusion, embryo survival does not seem significantly affected by IS, although numerical differences were great. Embryo development, however, was negatively affected in IS6 sows possibly due to a combination of high milk production, stress and lactational effects on uterine development.

Pulsatile release of luteinising hormone during the luteal phase in lactating and weaned sows

The present paper describes luteal phase luteinising hormone (LH) in sows that ovulated due to a limited nursing regimen (LN). The LN regimen was imposed either at Day 14 or at Day 21 of lactation. At ovulation, lactation was terminated (n = 8) or sows remained lactating throughout early pregnancy (n = 8). Blood samples were collected every 15 min for 8 h during the day, around Day 11 after ovulation. In addition, lactating sows were bled during the night, when piglets were allowed to suckle. The LH pattern was typical for the luteal phase, with one to five pulses per 8 h. The LH characteristics (frequency, base, average, pulse area) did not differ between lactating and weaned sows, except for the amplitude of LH pulses, which was higher in weaned sows compared with lactating sows (1.22 +/- 0.15 v. 0.76 +/- 0.11 ng mL(-1), respectively; P < 0.05). In lactating sows, average LH, basal LH and the frequency of LH pulses were significantly lower during the night, when piglets were allowed to suckle. The sage of lactation at which LN was imposed did not influence LH. In conclusion, it is unlikely that the small difference in LH explains the considerable difference between lactating and weaned sows in progesterone at Day 12 of pregnancy (24.1 +/- 1.3 v. 43.3 +/- 4.0 ng mL(-1), respectively; P < 0.01). Moreover, the difference in progesterone already exists during the early luteal phase (Day 0-10), when secretion of the corpora lutea is still independent of LH.

Cystic ovaries in intermittently-suckled sows: follicle growth and endocrine profiles

This paper presents follicle development and hormone profiles for sows with normal ovulation or cystic follicles during an intermittent-suckling (IS) regime that started at Day 14 of lactation. Sows were subjected to separation from their piglets during blocks of 6h or 12h. In total, 8 out of 52 sows developed cystic follicles; either full cystic ovaries (n=6) or partial ovulation (n=2). Increase in follicle size of these sows was similar to that of normal ovulating sows until pre-ovulatory size at Day 5 after the start of separation, but from then on became larger (P<0.05). LH surge was smaller or absent in sows that developed (partially) cystic ovaries (0.4 ± 0.1 vs 3.6 ± 0.3 ngmL(-1); P<0.01). Peak levels of oestradiol (E2) were similar but high E2 levels persisted in sows that developed (partly) cystic ovaries and duration of oestrus tended to be longer. The risk of developing (partly) cystic ovaries was higher when IS occurred in blocks of 6h versus 12h (33 vs 10%). In conclusion, the appearance of cystic ovaries at approximately Day 20 of ongoing lactation was related to an insufficient LH surge, as is also the case in non-lactating sows.