Network


Latest external collaboration on country level. Dive into details by clicking on the dots.

Hotspot


Dive into the research topics where R. Hafsia is active.

Publication


Featured researches published by R. Hafsia.


Blood Cells Molecules and Diseases | 2010

Structural analysis of two novel mutations in MCFD2 gene causing combined coagulation factors V and VIII deficiency

Hejer Elmahmoudi Abdallah; Emna Gouider; Nejla Stambouli; Mohamed Ben Amor; Asma Jlizi; Nejla Belhedi; Rim Sassi; Houssein Khodjet-El-Khil; B. Meddeb; R. Hafsia; Adel Hamza; Amel Benammar Elgaaied

Combined factor V and factor VIII deficiency (F5F8D) is a rare autosomal recessive bleeding disorder reported usually in the context of consanguinous marriage. F5F8D is characterized by mild-tomoderate bleeding and coordinate reduction in plasma FV and FVIII levels, as well as platelet FV level (OMIM 227300) [1]. The disease is caused by mutations in genes encoding lectin mannose binding protein (LMAN1) and multiple coagulation factor deficiency 2 (MCFD2), which are the components of the endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC-53) involved in the FV and FVIII intracellular transport [1, 2]. LMAN1 is a type-I integral membrane protein that was first described as a 53-kDa marker of the ERGIC [3], whereas MCFD2 is a soluble luminal protein


Pathologie Biologie | 2014

Diagnostic de lignée dans les leucémies aiguës : confrontation entre cytologie et immunophénotypage

N. Ben Salah; W. El Borgi; A. Chelbi; F. Ben Lakhal; Emna Gouider; H. Aounallah Skhiri; R. Hafsia

OBJECTIVE The determination of the cellular lineage in acute leukemia is a crucial step in the diagnosis and the later therapeutic conduct. In Tunisia, emerging country, some cases of acute leukemias are still treated on the basis of an only cytologic study because of lack of cytometry. Our objective is to realize a confrontation between cytology and flow cytometry in the diagnosis of AL and to analyze discrepancies. PATIENTS AND METHODS The study concerns 100 cases of AL. A second double-blind examination of the bone marrow smears of acute leukemias is realized by two cytologists and confronted to immunophenotyping. RESULTS In two cases of AML, flow cytometry reassigned lineage into T ALL and biphenotypic AL. In three cases of ALL the lineage was reassigned into undifferentiated acute leukemia (2 cases) and biphenotypic acute leukemia (1 case). Lineage was not established in four cases, immunophenotyping allowed the diagnosis of B ALL in 3 cases, and of biphenotypic acute leukemia in 1 case. In both cases of discrepant findings, flow cytometry allowed the diagnosis of biphenotypic acute leukemia in a case and of AML in the other one. CONCLUSION The cytological study remains insufficient in the diagnosis of lineage even with experimented cytologists. Immunophenotyping is essential in lineage assignment and reassignment.


Annales De Biologie Clinique | 2013

Immunophenotyping of B chronic lymphoproliferative syndromes (CLL excluded): confrontation with the histology

Wijden El Borgi; Nawel Ben Salah; Fatma Ben Lakhal; Lamia Makni; Emna Gouider; R. Hafsia

Immunophenotyping is a major tool for the diagnosis of the chronic lymphoïd leukaemia (CLL). Its interest remains limited in the classification of the other B chronic lymphoproliférative syndromes (B-CLPS). We evaluate the place of the flow cytometry (CMF) in the diagnosis and classification of the non CLL B-CLPS. The cases with Matutes score of 4 or more are excluded. A confrontation of the results to the histology is made. 28 cases of non CLL B-CLPS are diagnosed. CMF shows a κ monoclonal population in 15 cases and λ in 13 cases. A co-expression CD19+CD5 + is found in 11 cases concording with an atypic CLL or a mantel cell lymphoma in 6 cases with Matutes score of 3. In 5 cases, we concluded to non CLL B-CLPS (Matutes<3). The histology retained the diagnosis of a mantel cell lymphoma (4 cases), a SLVL (1 case) and an atypical LLC (1 case). CD5 is negative in 17 cases. In 5 cases, the diagnosis of hairy cell leukemia (HCL) is retained (CD 11c+ CD103+) and confirmed by the histology. The diagnosis of a marginal zone lymphoma is retained in 2 cases, a SLVL in 2 cases, a follicular lymphoma in 3 cases and prolymphocytes leukaemia in 1 case. Nine cases of non CLL B-CLPS were difficult to classify by histology. CMF is insufficient for the classification of most of the non CLL B-CLPS. Only the phenotype of the HCL is characteristic. The confrontation of the histology results remains essential.


Transfusion Clinique Et Biologique | 2014

Immunisation anti-érythrocytaire et anti-HLA au cours des hémoglobinopathies

N. Ben Salah; W. El Borgi; F. Ben Lakhal; M. Ben Mansour; Emna Gouider; Y. Gorgi; R. Bardi; B. Zoueri; R. Hafsia


Mediterranean Journal of Hematology and Infectious Diseases | 2011

TREATMENT OF ACUTE PROMYELOCYTIC LEUKEMIA WITH AIDA BASED REGIMEN

Ramzi Jeddi; Hela Ghedira; Ramzi Ben Amor; Yosr Ben Abdennebi; Kacem Karima; Zarrouk Mohamed; Hend Ben Neji; Lamia Aissaoui; Raihane Ben Lakhal; Naouel Ben Salah; Samia Menif; Zaher Belhadjali; Hela Ben Abid; Emna Gouider; R. Hafsia; Ali Saad; Pierre Fenaux; Balkis Meddeb


Haemophilia | 2009

Small insertion (c.869insC) within F13A gene is dominant in Tunisian patients with inherited FXIII deficiency due to ancient founder effect

H. El Mahmoudi; Mohamed Ben Amor; Emna Gouider; R. Horchani; R. Hafsia; K. Fadhlaoui; Balkis Meddeb; A. Hafsia; A. B. Ammar El Gaaied


Transfusion Clinique Et Biologique | 2013

Connaissances médicales en matière de sécurité immunologique en transfusion érythrocytaire en Tunisie : évaluation d’un CD-ROM d’auto-enseignement

N. Ben Salah; W. El Borgi; H. Aounallah Skhiri; F. Ben Lakhal; H. Mouelhi; B. Zoueri; Emna Gouider; R. Hafsia


Tunisie médicale | 2007

L'HEMOGLOBINE O ARAB : Etude à propos de 20 cas

R. Hafsia; Emna Gouider; Sinda Ben Moussa; Naouel Ben Salah; Wijdene Elborji; A. Hafsia


Pathologie Biologie | 2001

Syndromes de défibrination atypiques et leucémies aiguës à translocation t(9,22) ; à propos de deux observations

B. Meddeb; Sami Guermazi; R. Hafsia; H. Ben Abid; Emna Gouider; R. Ben Lakhal; Z. Bel Haj Ali; T. Ben Othman; R. Jeddi; Koussay Dellagi; A. Hafsia


Annales De Biologie Clinique | 2009

Suivi de la maladie résiduelle dans les leucémies aiguës par cytométrie en flux

S Besbes; Emna Gouider; N Ben Salah; Lamia Aissaoui; W El Borji; R. Hafsia

Collaboration


Dive into the R. Hafsia's collaboration.

Top Co-Authors

Avatar
Top Co-Authors

Avatar

Balkis Meddeb

The Chinese University of Hong Kong

View shared research outputs
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar

Koussay Dellagi

University of La Réunion

View shared research outputs
Top Co-Authors

Avatar
Researchain Logo
Decentralizing Knowledge