R. Hafsia
Tunis University
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Publication
Featured researches published by R. Hafsia.
Blood Cells Molecules and Diseases | 2010
Hejer Elmahmoudi Abdallah; Emna Gouider; Nejla Stambouli; Mohamed Ben Amor; Asma Jlizi; Nejla Belhedi; Rim Sassi; Houssein Khodjet-El-Khil; B. Meddeb; R. Hafsia; Adel Hamza; Amel Benammar Elgaaied
Combined factor V and factor VIII deficiency (F5F8D) is a rare autosomal recessive bleeding disorder reported usually in the context of consanguinous marriage. F5F8D is characterized by mild-tomoderate bleeding and coordinate reduction in plasma FV and FVIII levels, as well as platelet FV level (OMIM 227300) [1]. The disease is caused by mutations in genes encoding lectin mannose binding protein (LMAN1) and multiple coagulation factor deficiency 2 (MCFD2), which are the components of the endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC-53) involved in the FV and FVIII intracellular transport [1, 2]. LMAN1 is a type-I integral membrane protein that was first described as a 53-kDa marker of the ERGIC [3], whereas MCFD2 is a soluble luminal protein
Pathologie Biologie | 2014
N. Ben Salah; W. El Borgi; A. Chelbi; F. Ben Lakhal; Emna Gouider; H. Aounallah Skhiri; R. Hafsia
OBJECTIVE The determination of the cellular lineage in acute leukemia is a crucial step in the diagnosis and the later therapeutic conduct. In Tunisia, emerging country, some cases of acute leukemias are still treated on the basis of an only cytologic study because of lack of cytometry. Our objective is to realize a confrontation between cytology and flow cytometry in the diagnosis of AL and to analyze discrepancies. PATIENTS AND METHODS The study concerns 100 cases of AL. A second double-blind examination of the bone marrow smears of acute leukemias is realized by two cytologists and confronted to immunophenotyping. RESULTS In two cases of AML, flow cytometry reassigned lineage into T ALL and biphenotypic AL. In three cases of ALL the lineage was reassigned into undifferentiated acute leukemia (2 cases) and biphenotypic acute leukemia (1 case). Lineage was not established in four cases, immunophenotyping allowed the diagnosis of B ALL in 3 cases, and of biphenotypic acute leukemia in 1 case. In both cases of discrepant findings, flow cytometry allowed the diagnosis of biphenotypic acute leukemia in a case and of AML in the other one. CONCLUSION The cytological study remains insufficient in the diagnosis of lineage even with experimented cytologists. Immunophenotyping is essential in lineage assignment and reassignment.
Annales De Biologie Clinique | 2013
Wijden El Borgi; Nawel Ben Salah; Fatma Ben Lakhal; Lamia Makni; Emna Gouider; R. Hafsia
Immunophenotyping is a major tool for the diagnosis of the chronic lymphoïd leukaemia (CLL). Its interest remains limited in the classification of the other B chronic lymphoproliférative syndromes (B-CLPS). We evaluate the place of the flow cytometry (CMF) in the diagnosis and classification of the non CLL B-CLPS. The cases with Matutes score of 4 or more are excluded. A confrontation of the results to the histology is made. 28 cases of non CLL B-CLPS are diagnosed. CMF shows a κ monoclonal population in 15 cases and λ in 13 cases. A co-expression CD19+CD5 + is found in 11 cases concording with an atypic CLL or a mantel cell lymphoma in 6 cases with Matutes score of 3. In 5 cases, we concluded to non CLL B-CLPS (Matutes<3). The histology retained the diagnosis of a mantel cell lymphoma (4 cases), a SLVL (1 case) and an atypical LLC (1 case). CD5 is negative in 17 cases. In 5 cases, the diagnosis of hairy cell leukemia (HCL) is retained (CD 11c+ CD103+) and confirmed by the histology. The diagnosis of a marginal zone lymphoma is retained in 2 cases, a SLVL in 2 cases, a follicular lymphoma in 3 cases and prolymphocytes leukaemia in 1 case. Nine cases of non CLL B-CLPS were difficult to classify by histology. CMF is insufficient for the classification of most of the non CLL B-CLPS. Only the phenotype of the HCL is characteristic. The confrontation of the histology results remains essential.
Transfusion Clinique Et Biologique | 2014
N. Ben Salah; W. El Borgi; F. Ben Lakhal; M. Ben Mansour; Emna Gouider; Y. Gorgi; R. Bardi; B. Zoueri; R. Hafsia
Mediterranean Journal of Hematology and Infectious Diseases | 2011
Ramzi Jeddi; Hela Ghedira; Ramzi Ben Amor; Yosr Ben Abdennebi; Kacem Karima; Zarrouk Mohamed; Hend Ben Neji; Lamia Aissaoui; Raihane Ben Lakhal; Naouel Ben Salah; Samia Menif; Zaher Belhadjali; Hela Ben Abid; Emna Gouider; R. Hafsia; Ali Saad; Pierre Fenaux; Balkis Meddeb
Haemophilia | 2009
H. El Mahmoudi; Mohamed Ben Amor; Emna Gouider; R. Horchani; R. Hafsia; K. Fadhlaoui; Balkis Meddeb; A. Hafsia; A. B. Ammar El Gaaied
Transfusion Clinique Et Biologique | 2013
N. Ben Salah; W. El Borgi; H. Aounallah Skhiri; F. Ben Lakhal; H. Mouelhi; B. Zoueri; Emna Gouider; R. Hafsia
Tunisie médicale | 2007
R. Hafsia; Emna Gouider; Sinda Ben Moussa; Naouel Ben Salah; Wijdene Elborji; A. Hafsia
Pathologie Biologie | 2001
B. Meddeb; Sami Guermazi; R. Hafsia; H. Ben Abid; Emna Gouider; R. Ben Lakhal; Z. Bel Haj Ali; T. Ben Othman; R. Jeddi; Koussay Dellagi; A. Hafsia
Annales De Biologie Clinique | 2009
S Besbes; Emna Gouider; N Ben Salah; Lamia Aissaoui; W El Borji; R. Hafsia