R.K. Ameta

Preparation and characterization of bionanoemulsions for improving and modulating the antioxidant efficacy of natural phenolic antioxidant curcumin

An attempt to augment the antioxidant ability of curcumin, a natural phenolic compound via encapsulation in nontoxic nanoemulsions, has been made. Ethanol and glycerol catalyzed curcumin encapsulated nanoemulsions using cottonseed oil with mild cationic, anionic and nonionic surfactants have been prepared. The prepared bionanoemulsions were found to possess characteristic particle diameter between 100 and 900nm. The curcumin encased bionanoemulsions have been further screened for antioxidant efficacy with free radical 2, 2-diphenyl-1-picrylhydrazyl that enabled a radical scavenging activity of more than 90% as compared to pure curcumin. Alongside, the reduced surface tension and increased viscosity of curcumin encapsulated bionanoemulsions within 38.37-52.85mN/m and 0.7947-1.042mPa-s have quantitatively verified curcumin dispersion and interaction within the continuous medium, enabled via thermodynamic shift.

Synthesis and structure–activity relationship of benzylamine supported platinum(IV) complexes

A series of benzylamine derivative (BAD) supported platinum(IV) complexes (PtCl4(BADs)2) have been synthesized. The complexes were tested in vitro against the MCF-7 cell line, and the 4-fluoro and 4-chloro containing complexes expressed impressive anticancer activities. Their DNA binding nature for a structure–activity relationship (SAR) study was investigated with physicochemical-indicators (PCI) which categorized them as good intercalators for host–guest chemistry. A mechanism for drug efficacy is proposed by analysis of the resultant viscosity and surface tension of PtCl4(BADs)2–DNA solutions named as the drug-friccohesity interaction (DFI). The complexes have shown significant antioxidant activity, determined on the basis of free radical scavenging effects due to their terminating action against the reactive species.

Synthesis, characterization, EDX, thermal, antioxidant, antibacterial, topographical, and gas adsorption studies of supramolecular tetraammoniumplatinate

Reaction of K2PtCl4 with N-alkyl-N-benzyl-N,N-dimethyl ammonium chloride (n = 8, 10, 12 and 14 for alkyl) yielded supramolecular bis(benzyldimethylalkylazaniumyl) tetrachloroplatinumdiuide (metallobenzalkonium8 (MBK8), MBK10, MBK12, MBK14) characterized with spectroscopic and energy dispersive X-ray techniques. X-ray difraction powder analysis was performed which confirmed the crystalline nature of the compound. UV/Vis study performed in dimethylformamide, dimethylsulphoxide (DMSO)/H2O, and DMSO/phosphate buffers under physiological conditions show stability of the supramolecular units, with [PtCl4]2−. Zeta potential with particle size distribution of MBK8 obtained with DLS-confirmed agglomeration of the coordinate units. Thermal analysis by differential scattering calorimetric showed an effect of alkyl chain on their heat retention. For MBK8, topographical analysis and specific surface area were investigated with atomic force microscopy and nitrogen adsorption method, illustrating an outstanding alignment in arrays of ionic units, and an extent of apparent cross-sectional areas, respectively. Antioxidant and antibacterial activities were determined with 1–2,5-diphenyl-2-picrylhydrazyl-scavenging effect and Gram-positive and Gram-negative bacteria, respectively. The compounds exhibited activities for both scavenging and bacteria.

Synthesis, Characterization, Anticancer, DNA Binding and Antioxidant Studies of Benzylamine Supported Pd (II) Complex

A benzylamine supported palladium (II) complex has been synthesized and characterized using different spectroscopic techniques, such as FTIR, UV/Vis., 1H, 13C NMR and LCMS. The semi crystalline nature of the complex was investigated through powder XRD and SAED pattern analysed by HRTEM. The in vitro biological study against breast cancer cell lines MCF-7 and MDA-MB-231 was not much effective but their strong DNA binding activity (DBA) compared to free ligand suggested them as a good DNA binder and makes them interesting against other cancerous cell lines. The DBA further supported by physicochemical studies such as surface tension and viscosity of complex+DNA which inferred the disruption and intercalation of DNA and complex. The scavenging activity (SA) also proves the anticancer nature of complex, in this context, the scavenging activity of complex was determined through DPPH• free radical and the result indicates good antioxidant behaviour of complexes.

Biological Impact of Pd (II) Complexes: Synthesis, Spectral Characterization, In Vitro Anticancer, CT-DNA Binding, and Antioxidant Activities

A new series of Pd (II) complexes of methyl substituted benzylamine ligands (BLs) has been synthesized and characterized via spectroscopic techniques such as UV/Vis. FTIR, LCMS, 1H, and 13C NMR. The UV/Vis study in DMSO, DMSO + water, and DMSO + PBS buffer (pH = 7.2) confirmed their molecular sustainability in liquids. Their in vitro anticancer activity against breast cancer cell lines such as MCF-7 and MDA-MB-231 makes them interesting for in vivo analysis. Their stronger DNA binding activity (DBA) compared with free ligand suggested them as a good DNA binder. DBA was further confirmed by physicochemical studies such as surface tension and viscosity of complex + DNA which inferred the disruption of DNA and intercalation of complexes, respectively. Their % binding activity, % disruption of DNA base pairs (DNABP), and % intercalating strength are reported in this paper for the first time for better understanding of DNA binding mechanism. Along with this, their scavenging activity (SA) determined through DPPH free radical and the results indicate good antioxidant behaviour of complexes.

From Synthesis to Biological Impact of Pd (II) Complexes: Synthesis, Characterization, and Antimicrobial and Scavenging Activity

The Pd (II) complexes with a series of halosubstituted benzylamine ligands (BLs) have been synthesized and characterized with different spectroscopic technique such as FTIR, UV/Vis, LCMS, 1H, and 13C NMR. Their molecular sustainability in different solvents such as DMSO, DMSO : H2O, and DMSO : PBS at physiological condition (pH 7.2) was determined by UV/Vis spectrophotometer. The in vitro antibacterial and antifungal activities of the complexes were investigated against Gram-positive and Gram-negative microbes and two different fungi indicated their significant biological potential. Additionally, their antioxidant activity has been analyzed with DPPH• free radical through spectrophotometric method and the result inferred them as an antioxidant. The stronger antibacterial and antioxidant activities of the synthesized complexes suggested them as a stronger antimicrobial agent. Our study advances the biological importance of palladium (II) amine complexes in the field of antimicrobial and antioxidant activities.