R. K. Juneja

Towards construction of a canine linkage map: Establishment of 16 linkage groups

1Norwegian Kennel Klub and Department of Morphology, Genetics and Aquatic Biology, Section of Genetics, P.O. Box. 8146 Dep., N-0033 Oslo, Norway ZDepartment of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Box 7023, 750 07 Uppsala, Sweden 3Department of Animal Science and Animal Health, Division of Animal Genetics, The Royal Veterinary and Agricultural University, Btilowsvej, 13, DK-1870, Fredriksberg C, Copenhagen, Denmark ~The Department of Biochemistry, University of Leicester, University Road, Leicester, LE1 7RH, UK 5Centre for Preventive Medicine, Animal Health Trust, PO Box 5, Newmarket, Suffolk CB8 7DW, UK 6Medical Technology and Medicine, Michigan State University, B228 Life Science, East Lansing, Michigan 48824-1317, USA 7Institute of Animal Breeding, University of Berne, Bremgartenstrasse 109 a, 3012 Berne, Switzerland

A major locus (RN) affecting muscle glycogen content is located on pig chromosome 15.

The RN locus in pigs has a major effect on the amount of stored glycogen in white muscle and affects meat quality. The fully dominant RN− allele, associated with high glycogen content, occurs in the Hampshire breed. We have mapped the RN locus using a large half-sib family comprising one heterozygous RN− / rn+ Hampshire boar mated to homozygous rn+/rn+ Swedish Landrace × Swedish Yorkshire sows. The segregation at the RN locus was inferred from data on glycolytic potential and residual glycogen in white muscle which both showed clear bimodal distributions. Highly significant evidence for genetic linkage was obtained against microsatellite markers on Chromosome (Chr) 15. Multipoint analysis revealed the order Sw1111- 8.0 -S0088- 10.6 -RN- 4.8 -Sw936,Sw906 (recombination estimates are given as Kosambi cM). Comparative mapping data imply that the human homolog of RN is located on Chr 2q.

A within-litter comparison of the three halothane genotypes. 1. Piglet performance and effects of transportation and amperozide treatment at 12 weeks of age.

The aim of the investigation was to compare the three Hal genotypes within litter, with respect to: (1) piglet performance; (2) effects on agonistic behaviour and weight gain of transport and amperozide treatment at 12 weeks of age. The animals were offspring of heterozygous boars and sows, and the Hal genotype was revealed by the halothane test combined with blood typing. One hundred and twenty pigs, kept in groups of four (1 NN, 2 Nn and 1 nn from different litters), were allotted to the four treatments in an experiment with a 2 × 2 factorial design: with and without transport (5 h) × with and without amperozide treatment. Each transport treatment comprised 15 pens and the animals in seven of these were treated with amperozide. Piglet weight at birth and at 9 weeks did not differ between the Hal genotypes. Amperozide treatment decreased the agonistic behaviour, but the effects on weight gain were inconsistent. Overall, daily weight gain in the first 13 days after the transport/amperozide treatments did not differ, neither between treatment groups nor between Hal genotypes, but interactions between Hal genotype, sex and amperozide treatment were found. Gilts attacked more than castrates, and interactions between Hal genotype and sex were indicated for agonistic behaviour. Creatine kinase (CK) activity was higher in nn compared with NN and Nn pigs. Transport increased the CK activity.

Association of the transferrin locus on chromosome 13 with early body weights in pigs

SUMMARY The effect of the genotypes of five different blood protein loci (α1B-glycoprotein, A1BG; glucose phosphate isomerase, GPI; phosphogluconate dehydrogenase, PGD; postalbumin 1A, PO1A; transferrin, TF) on early body-weight traits was studied in one large population of Swedish Yorkshire breed pigs. A highly significant association was observed, between the transferrin genotypes and the piglet body weights, at 6 and 9 weeks of age. The TF BB type pigs were heavier than those of TF AB types at 3, 6, and 9 weeks of age, by 130, 340, and 370 g, respectively. In the light of previously published data, it was discussed that TF is an additional chromosome 13 marker that may affect early body weights in pigs. The other four loci studied, located on chromosomes 6 and 7, did not show any significant effect. ZUSAMMENFASSUNG: Zusammenhänge zwischen Transferrinlocus an Chromosom 13 und Ferkelgewichten Die Wirkung von fünf verschiedenen Blutproteinloci (αB-Glykoprotein, A1BG; Glukose Phosphat Isomerase, GPI; Phosphoglukonat Dehydrogenase, PGD; Postalbumin 1A, PO1A; Transferrin, TF) auf Ferkelwichte wurde bei Schwedischen Yorkshire Schweinen untersucht. Der Zusammenhang zwischen Transferrin Genotypen und 6 und 9 Wochen Gewichten war hochsignifikant, TF BB Ferkel waren bei 3, 6 und 9 Wochen Alter um 130, 340 und 370 g schwerer als TF AB Ferkel. In zusammenhang mit früheren Studien wird TF als ein weiterer Chromosom 13 Marker für Ferkelgewicht erörtert. Die anderen vier Loci an Chromosomen 6 bzw. 7 zeigten keine signifikante Wirkung.