R. L. Riley

Influence of Body Size on Gaseous Nitrogen Elimination During High Oxygen Breathing.

Summary 1. The volume of nitrogen excreted from the body by way of the lungs, when a subject breathes pure oxygen for short periods, is found to vary with the size of the individual. 2. The nitrogen excretion during 7 minutes of oxygen breathing has been determined in 30 normal individuals of various ages and sizes. The values obtained have been correlated with body weight and with surface area. Coefficients of correlation and regression equations are given.

Isolation and initial characterization of a Schizosaccharomyces pombe mutant exhibiting temperature-dependent radiation sensitivity due to a mutation in a previously unidentified rad locus

SummaryWe have isolated a mutant of the yeast Schizosaccharomyces pombe which exhibits sensitivity to UV light when grown at either 30° or 37°C, as compared to the parental wild-type strain. This increased sensitivity is more pronounced when cells are grown at 37°C. The mutant is also sensitive to 18 MeV electrons at the high temperature. Tetrad analysis of spores generated by crossing the mutant and a Rad+ strain revealed that sensitivity to both types of radiation cosegregate 2:2, relative to wild-type resistance, indicating that a single altered chromosomal locus is responsible for the radiation sensitivities observed. In addition, analysis of spores resulting from crosses between the mutant and all other known S. pombe rad mutants indicates that the temperature-dependent sensitivity described in this report is mediated by a mutation in a previously unidentified rad locus.