R. M. Teclaw

Leaf and canopy conductance in aspen and aspen-birch forests under free-air enrichment of carbon dioxide and ozone

Increasing concentrations of atmospheric carbon dioxide (CO2) and tropospheric ozone (O3) have the potential to affect tree physiology and structure, and hence forest feedbacks on climate. Here, we investigated how elevated concentrations of CO2 (+45%) and O3 (+35%), alone and in combination, affected conductance for mass transfer at the leaf and canopy levels in pure aspen (Populus tremuloides Michx.) and in mixed aspen and birch (Betula papyrifera Marsh.) forests in the free-air CO2-O3 enrichment experiment near Rhinelander, Wisconsin (Aspen FACE). The study was conducted during two growing seasons, when steady-state leaf area index (L) had been reached after > 6 years of exposure to CO2- and O3-enrichment treatments. Canopy conductance (g(c)) was estimated from stand sap flux, while leaf-level conductance of sun leaves in the upper canopy was derived by three different and independent methods: sap flux and L in combination with vertical canopy modelling, leaf 13C discrimination methodology in combination with photosynthesis modelling and leaf-level gas exchange. Regardless of the method used, the mean values of leaf-level conductance were higher in trees growing under elevated CO2 and/or O3 than in trees growing in control plots, causing a CO2 x O3 interaction that was statistically significant (P < or = 0.10) for sap flux- and (for birch) 13C-derived leaf conductance. Canopy conductance was significantly increased by elevated CO2 but not significantly affected by elevated O3. Investigation of a short-term gap in CO2 enrichment demonstrated a +10% effect of transient exposure of elevated CO2-grown trees to ambient CO2 on g(c). All treatment effects were similar in pure aspen and mixed aspen-birch communities. These results demonstrate that short-term primary stomatal closure responses to elevated CO2 and O3 were completely offset by long-term cumulative effects of these trace gases on tree and stand structure in determining canopy- and leaf-level conductance in pure aspen and mixed aspen-birch forests. Our results, together with the findings from other long-term FACE experiments with trees, suggest that model assumptions of large reductions in stomatal conductance under rising atmospheric CO2 are very uncertain for forests.

The impact of forest structure on near-ground temperatures during two years of contrasting temperature extremes

The thermal environment of clear-cut, partially cut, and uncut forest sites in northern Wisconsin are examined for a warm year and a cool year. Temperatures at 0.5 m above and 0.05m below ground, as well as base 5 degree C heat sums are computed for each site between May and September and differences between cut and uncut sites compared for the 2 years. differences in average and minimum air temperature and soil temperature are less than instrumental error, E = 0.3 degree C. Maximum air temperature differences between the clear-cut and uncut sites drop from 5.7 degree C in the cool year to 4.7 degree C in the warm year, while the difference for the partial cut drops from 3.2 to 2.7 degree C. The results suggest that studies of tree growth or forest development and climate change should consider the effects of forest structure on changes in daily extreme temperatures.

Stomatal uptake of O3 in aspen and aspen-birch forests under free-air CO2 and O3 enrichment

Rising atmospheric carbon dioxide (CO2) may alleviate the toxicological impacts of concurrently rising tropospheric ozone (O3) during the present century if higher CO2 is accompanied by lower stomatal conductance (gs), as assumed by many models. We investigated how elevated concentrations of CO2 and O3, alone and in combination, affected the accumulated stomatal flux of O3 (AFst) by canopies and sun leaves in closed aspen and aspen-birch forests in the free-air CO2-O3 enrichment experiment near Rhinelander, Wisconsin. Stomatal conductance for O3 was derived from sap flux data and AFst was estimated either neglecting or accounting for the potential influence of non-stomatal leaf surface O3 deposition. Leaf-level AFst (AFst(l)) was not reduced by elevated CO2. Instead, there was a significant CO2 x O(3) interaction on AFst(l), as a consequence of lower values of gs in control plots and the combination treatment than in the two single-gas treatments. In addition, aspen leaves had higher AFst(l) than birch leaves, and estimates of AFst(l) were not very sensitive to non-stomatal leaf surface O3 deposition. Our results suggest that model projections of large CO2-induced reductions in gs alleviating the adverse effect of rising tropospheric O3 may not be reasonable for northern hardwood forests.

Microclimatic variation between managed and unmanaged northern hardwood forests in Upper Michigan, USA.

Temperature, light, wind, and precipitation were measured in the understory of managed and unmanaged northern hardwood forests in the Upper Peninsula of Michigan from 1995 through 2001. These measurements provide a baseline of information to compare the microclimate under managed and unmanaged conditions. Extreme climatic events may influence growth and development of forests.

Effects of elevated atmospheric CO2 and tropospheric O3 on tree branch growth and implications for hydrologic budgeting

The forest hydrologic budget may be impacted by increasing CO(2) and tropospheric O(3). Efficient means to quantify such effects are beneficial. We hypothesized that changes in the balance of canopy interception, stem flow, and through-fall in the presence of elevated CO(2) and O(3) could be discerned using image analysis of leafless branches. We compared annual stem flow to the results of a computerized analysis of all branches from the 2002, 2004, and 2006 annual growth whorls of 97 ten-year-old trees from the Aspen Free-Air CO(2) and O(3) Enrichment (Aspen FACE) experiment in Rhinelander, WI. We found significant effects of elevated CO(2) and O(3) on some branch metrics, and that the branch metrics were useful for predicting stem flow from birch, but not aspen. The results of this study should contribute to development of techniques for efficient characterization of effects on the forest hydrologic budget of increasing CO(2) and tropospheric O(3).