R. M. W. Dixon

Effects of Phyllanthus plant extracts on duck hepatitis B virus in vitro and in vivo

The effects of extracts of five Australian Phyllanthus species (P. hirtellus, P. gunnii, P. gasstroemii, P. similis and P. tenellus), other plant extracts and the antiviral drug foscarnet on duck hepatitis B virus (DHBV) endogenous DNA polymerase (DNAp) activity were compared. All 5 Phyllanthus species caused 50% inhibition at concentrations of dry weight between 350-800 micrograms/ml, which is comparable with the effect described for P. amarus on the DNAp of human and woodchuck hepatitis B viruses. Incubation of P. hirtellus with 100 ID50 DHBV neutralized infection. However, neither P. gasstroemi extract, given by intraperitoneal injection (i.p.) at a dose of 20 mg/kg 3 times per week to ducklings early in the incubation period, or P. hirtellus extract, given to established DHBV carrier ducklings, prevented or eliminated infection.

The use of ampligen alone and in combination with ganciclovir and coumermycin A1 for the treatment of ducks congenitally-infected with duck hepatitis B virus

Ampligen, a known immunomodulator and interferon inducer, was used alone and in combination with other antiviral agents to treat ducks congenitally-infected with duck hepatitis B virus. These antiviral agents included the conventional nucleoside analogue ganciclovir and the prokaryotic DNA gyrase B inhibitor coumermycin A1. When used alone, ampligen decreased the amount of serum and liver viral DNA, but had no effect on circulating duck hepatitis B surface antigen (DHBsAg). In combination with ganciclovir, the antiviral effect appeared at least additive with a greater inhibition of viral DNA replication within the liver. The combination of ampligen with coumermycin A1 also resulted in inhibition of viral replication but to a lesser extent than ampligen alone. When all three agents were used together, viral DNA replication was again inhibited, but as with previous treatment regimes, serum DHBsAg levels remained unchanged. At the end of the treatment period for all regimes, analysis of viral DNA forms in the liver showed that the viral relaxed circular and supercoiled DNA forms had persisted. Within 1 week of cessation of therapy, viral replication had often returned to pre-treatment levels. Interferon-like activity was detected in the sera of the majority of the treated ducks during the ampligen therapy, but no clear relationship between the presence of interferon and antiviral effect could be established. These observations in the duck hepatitis B model may provide a rational basis for the use of combinations of antiviral and immunomodulatory regimes for the management of chronic hepatitis B infection in man.

Australian Aboriginal words in English : their origin and meaning

This extensive reference provides authoritative information about the history of over 400 words from Aboriginal languages, offering the fullest available information about their Aboriginal background and Australian English history. The book begins with a general history of the 250 Australian aboriginal languages, including profiles of the languages that have been most significant as sources for borrowing. The words are then grouped according to subject: birds, fish, edible flora, dwellings, etc., with each work listed in a dictionary-style entry. The book concludes by addressing how words changed in English, and discusses English words that have since been adopted into Aboriginal languages.

Yir-Yoront lexicon : sketch and dictionary of an Australian language

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Cellular immune response of ducks to duck hepatitis B virus infection

Duck hepatitis B virus (DHBV) has been a useful model for hepadnavirus infection. There have been few studies on immunity to DHBV and none describing the cell‐mediated immune response by acute and chronically infected ducks. A duck hepatitis B antigen‐specific blastogenesis assay was used to measure DHBV antigen‐specific responses of duck peripheral blood (PBMC) and splenic mononuclear cells (SMCs) from uninfected control ducks, ducks acutely or chronically infected with DHBV, and ducks immune to DHBV. A comparison of the group mean responses by PBMC to DHBV surface antigen (DHBsAg) found that the immune group was significantly different to the other three groups (controls or unexposed, P < 0.0001; acutely infected, P < 0.01; chronically infected, P < 0.01). The responses to DHBsAg by PBMC of the acute group (P < 0.01) were significantly different also to that of the unexposed group. For DHBV core antigen (DHBcAg), significant differences in the responses were found between immune ducks and unexposed (P < 0.0005) and acutely infected (P < 0.05) groups. The SMC showed a significant difference between unexposed ducks and immune ducks (P < 0.05) in the group mean responses to DHBsAg. The responses to DHBcAg were significantly different between the immune group and the acute (P < 0.01) and unexposed (P < 0.01) groups. The group mean of unexposed ducks was also significantly different to that of acutely infected ducks (P < 0.01). This study indicates that the cellular immune response in immune animals differs from acutely and chronically infected ducks. Further studies of these differences may provide some explanations for the differing outcomes of DHBV infection. J. Med. Virol. 58:19–25, 1999.

Travelling in the West: The Writing of Amitav Ghosh

This paper is in one sense a survey of the increasingly substantial body of writing by the Indian novelist and anthropologist Amitav Ghosh. Bom in Calcutta in 1956, Ghosh has a PhD in social anthropology from Oxford and has taught in both Indian and American universities. His oeuvre now includes two novels, The Circle of Reason (1986) and The Shadow Lines (1988), the ethnography In an Antique Land (1993) and a number of essays, notably the scholarly article &dquo;The Slave of MS.H.6&dquo;, published in Subaltern Studies in 1992. My interest in Ghosh’s work arose initially from a growing concern with the way theories of colonial discourse have become globalized, while the practice of a good deal of post-colonial criticism has become overly theoreticized and predictable. Ghosh’s training in historical and anthropological research, his eschewing of grand theoreticist gestures and his links with the Subaltern Studies project, make his work an interesting site around which current arguments in post-colonial theory can be conducted.

Quantification of infectious duck hepatitis B virus by radioimmunofocus assay

A simple method is described for the precise quantification of infectious duck hepatitis B virus (DHBV) in cell culture, using a radioimmunofocus assay (RIFA). Primary duck hepatocyte cell cultures were infected with serial dilutions of viral samples as for a plaque assay, but then maintained with liquid overlay medium. After incubation for up to 14 days, cell monolayers were fixed with acetone, then stained with a monoclonal antibody to DHBV L protein followed by secondary antibody labelled with I. Foci of infection (representing individual infectious particles in the inoculum) were detected by autoradiography. The number of foci recovered was increased by addition of dimethyl sulphoxide to culture medium, but was not appreciably altered by the use of semi‐solid medium. The titre of virus suspensions determined by RIFA correlated well with titration in ducklings. The RIFA is a useful method for titration of DHBV, as it has a wide dynamic range and is well suited to parallel titration of large numbers of samples. This assay will have wide use for the analysis of DHBV growth kinetics, antiviral efficacy, and virus inactivation procedures. J. Med. Virol. 52:354–361, 1997.

Making new words : morphological derivation in English

Making New Words provides a detailed study of the 200 or so prefixes and suffixes which create new words in todays English. Alongside a systematic discussion of these forms, Professor Dixon explores and explains the hundreds of conundrums that seem to be exceptions to general rules. Why, for instance, do we say un-distinguished (with prefix un-) but in-distinguishable (with in-); why un-ceasing but in-cesssant? Why, alongside gold-en, do we say silver-y (not silver-en)? Why is it wood-en (not wood-ic) but metall-ic (not metall-en)? After short preliminary chapters, which set the scene and outline the criteria employed, there are accounts of the derivation of negative words, of other derivations which do not change word class, on making new verbs, new adjectives, new nouns, and new adverbs. The final chapter deals with combinations of suffixes, of prefixes, and of the two together. Within each chapter, derivational affixes are arranged in semantic groups, the members of which are contrasted with respect to meaning and function; for example, child-less and child-free. For each affix there is an account of its genetic origin (from Old English, Greek, Latin, French, and so on), its phonological form and implications for stress placement, the roots it can be attached to (and why), and how its range of meanings has developed over the centuries. The book is written in the authors accustomed style - clear and well-organised, with easy-to-understand explanations. The exposition is illustrated by examples, ranging from Shakespeare, W. S. Gilbert, and modern novels to what was heard on the radio. It will be an invaluable text and sourcebook for scholars and students of the English language and of general linguistics, from undergraduate level upwards. The many fascinating facts presented here, in such a lucid and accessible manner, will also appeal to the general reader interested in picking to pieces the English language to see how it works.

ANTIGEN-SPECIFIC BLASTOGENESIS ASSAYS FOR DUCK HEPATITIS B VIRUS USING DUCK PERIPHERAL BLOOD AND SPLENIC MONONUCLEAR CELLS

An antigen-specific lymphoblastogenesis assay for duck hepatitis B surface antigen (DHBsAg) and duck hepatitis B core antigen (DHBcAg) was developed using mononuclear cells from the peripheral blood (PBMC) or spleens (SMC) of immune ducks. Optimal culture conditions for the assay were determined by testing a number of variables, including antigen concentration, cell numbers/well, and the day of harvest. The specificity of the assay was assessed. The assay used 10% pooled duck serum supplement, and 8 x 10(5) cells/well for PBMC or 5 x 10(5) cells/well for SMC. The optimum antigen concentration ranged from 0.01 to 0.1 microgram/ml for both DHBsAg and DHBcAg. Maximum antigen-specific blastogenesis occurred between 4 to 7 days after establishment of the culture. The use of PHA (10 micrograms/ml) mitogenesis could predict the optimal cell numbers/well for antigen-specific blastogenesis. The assay demonstrated specific responses by immune ducks compared with those of unexposed ducklings and adult ducks (for DHBsAg P < 0.001; DHBcAg P < 0.05). For immune ducks, PBMC from all 8 ducks responded to DHBsAg, however, cells from only 4 of 7 immune ducks, responded to DHBcAg. Splenic mononuclear cells from all immune ducks responded to either DHBsAg or DHBcAg or both antigens.

Categories of the noun phrase in Jarawara

The head of an NP is taken to be that component which determines the categories of the NP as a whole. First impression of an NP in Jarawara (Arawa family, Brazil) involving inalienable possession (e.g. o-mano ‘my arm’) is that there is conflict of criteria concerning what is head. The gender of the NP, for verbal suffix agreement, is determined by the possessor (here I sg prefix o -), suggesting that this should be taken to be head. But the whole NP counts as 3rd person, for verbal prefix agreement, suggesting that the possessed noun ( mano ‘arm’) should be taken as head. Furthermore, the NP counts as inanimate. Detailed analysis shows that there is in fact no conflict. All NPs in this language are 3rd person (1st and 2nd persons being confined to head marking within the predicate and functioning as possessors within an NP, not as full NPs). And all NPs involving inalienable possession count as inanimate. The only variable is gender, which is determined by the possessor; plainly, this is the unequivocal head of the NP.