R. N. Pandey

EFFECT OF PACLOBUTRAZOL AND PUTRESCINE ON ANTIOXIDANT ENZYMES ACTIVITY AND NUTRIENTS CONTENT IN SALT TOLERANT CITRUS ROOTSTOCK SOUR ORANGE UNDER SODIUM CHLORIDE STRESS

The effects of paclobutrazol (PBZ) and putrescine (Put) on antioxidant enzymes activity, proline contents and nutrients uptake were studied on salt tolerant citrus rootstock sour orange. Six-month-old nucellar seedlings grown in pots and subjected to three levels of PBZ and two levels each of salinity and Put for 90 days. Seedlings treated with PBZ or Put alone or in combination had higher anti-oxidant enzymes activities, accumulation of proline and nutrients contents like potassium (K+) and calcium (Ca2+) under both saline and non-saline conditions. Further, application of PBZ or Put alone or in combination also reduced the accumulation of both Na+ and Cl− ions in leaves and roots in NaCl stressed seedlings. A combined application of 250 mg L−1 PBZ and 50 mg L−1 Put proved to be more effective in improving proline and Ca2+ content and restricting accumulation of Na+ ions in leaf tissues.

Development of Critical Values for the Leaf Color Chart, SPAD and Fieldscout CM 1000 for Fixed Time Adjustable Nitrogen Management in Aromatic Hybrid Rice (Oryza sativa L.)

The nitrogen (N) requirement of hybrid rice is generally greater than in conventional rice varieties. Recommendations for N monitoring at regular intervals of 7–10 days through leaf greenness are available, but farmers are accustomed to apply fertilizer N at selected growth stages only. An inexpensive leaf color chart (LCC) and nondestructive chlorophyll meters were evaluated for site-specific N management strategy in world’s first aromatic rice hybrid PRH-10 at the Indian Agricultural Research Institute, New Delhi. Two field experiments were conducted on PRH-10 with four levels of N (0, 70, 140, and 210 kg ha−1) during June–October of 2010 and 2011 to determine the LCC, soil–plant analysis development (SPAD), and Fieldscout CM 1000 (CM 1000) values for achieving economic optimum grain yield at three critical growth stages (tillering, panicle initiation, and flowering). Quadratic regression between N levels and grain yield were used to determine economic optimum grain yield (6427 kg ha−1 in 2010 and 6399 kg ha−1 in 2011) corresponding to optimum economical dose of 151 kg N ha−1 (2010) and 144 kg N ha−1 (2011). Nitrogen concentration in fully expanded youngest leaf correlated significantly (P < 0.01) and positively with LCC score, SPAD value, CM 1000 value, and total chlorophyll concentration at tillering, panicle initiation, and flowering for both years. The critical LCC score, SPAD, CM 1000 values, chlorophyll concentration, and leaf N concentration obtained were at tillering 4.4, 42.3, 285, and 2.16 mg g−1 fresh weight and 3.29%; at panicle initiation 4.4, 43.0, 276, and 2.16 mg g−1 fresh weight and 3.02%; and at flowering 4.5, 41.7, 270, and 2.05 mg g−1 fresh weight and 2.83%, respectively. Corrective N application should be done when observed leaf N indicator values at a particular growth stage reach or go below the critical values.

Manganese deficiency in wheat genotypes: Physiological responses and manganese deficiency tolerance index

ABSTRACT Manganese (Mn) deficiency limits wheat productivity on sandy loam, calcareous and alkaline soils cropped with rice. Variation of wheat genotypes to sustain production and Mn use from Mn deficient condition was investigated to screen efficient genotypes. Forty-seven diverse wheat genotypes were evaluated on Mn sufficient (0.195 µM) and Mn deficient (0 µM) nutrient solution to elucidate physiological basis of Mn deficiency tolerance and to develop manganese deficiency tolerance index (MDTI). Shoot dry weight and mean Mn accumulation was 136.7% and 76.5% enhanced when Mn nutrition was improved, respectively. Efficient genotypes under limited Mn had lower root length/shoot weight ratio but higher relative shoot growth rate with higher shoot demand on root which reflected higher Mn influx. Genotypes were classified as tolerant (>0.66), semi-tolerant (0.33–0.66) and sensitive (<0.33) on the basis of MDTI (0–1 scale). Manganese efficient genotypes are most desirable for sustainable production of wheat under low Mn.

Physiological basis of iron chlorosis tolerance in rice (Oryza sativa) in relation to the root exudation capacity

ABSTRACT Micronutrient deficiency in cultivable soil, particularly that of iron (Fe) and zinc (Zn), is a major productivity constraint in the world. Low Fe availability due to the low solubility of the oxidized ferric forms is a challenge. An experiment was, thus, executed to assess the performance of eight genetically diverse rice genotypes on Fe-sufficient (100 µM) and Fe-deficient (1 µM) nutrient solution, and their ability to recover from Fe deficiency was measured. Fe efficiency under Fe deficiency in terms of biomass production showed a significant positive correlation with the root release of phytosiderophore (PS) (R2 = 0.62*). This study shows that the Fe deficiency tolerance of Pusa 33 was related to both a high release of PS by the root and an efficient translocation of Fe from the root to the shoot as the Fe–PS complex, which could be useful for improving the Fe nutrition of rice particularly under aerobic conditions.