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Dive into the research topics where R. O. C. Silva is active.

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Featured researches published by R. O. C. Silva.


Animal Biotechnology | 2018

Extender Supplementation with Antioxidants Selected after the Evaluation of Sperm Susceptibility to Oxidative Challenges in Goats

D. S. R. Angrimani; R. O. C. Silva; J. D. A. Losano; A. Dalmazzo; Roberta Harue Tsunoda; E. G. A. Perez; P. A. A. Góes; V. H. Barnabe; M. Nichi

ABSTRACT This study aimed to detect the most deleterious ROS for goat sperm and then supplemented the extender with a proper antioxidant. For this, 12 adult goats (aged 1–7) were used. Fresh samples were submitted to challenge with different ROS (superoxide anion, hydrogen peroxide, and hydroxyl radical) and malondialdehyde (MDA—toxic product of lipid peroxidation). After experiment 1, sperms were cryopreserved in extenders supplemented to glutathione peroxidase (Control: 0 UI/mL; GPx1: 1 UI/mL; GPx5: 5 UI/mL, and GPx10: 10 UI/mL) and catalase (Control: 0 UI/mL; CAT60: 60 UI/mL; CAT120: 120 UI/mL, and CAT240: 240 UI/mL). Each sample was evaluated by motility, plasma membrane integrity (eosin/nigrosin), acrosome integrity (fast green/rose bengal), sperm morphology, assay of the sperm chromatin structure, mitochondrial activity (3,3-diaminobenzidine), and measurement of lipid peroxidation (thiobarbituric acid reactive substances [TBARS]). It was possible to observe a mitochondrial dysfunction (DAB—Class IV) and low membrane integrity after hydrogen peroxide action. However, the high rates of TBARS were observed on hydroxyl radical. CAT240 presents the lower percentage of plasma membrane integrity. It was possible to attest that hydrogen peroxide and hydroxyl radical are the more harmful for goat sperm. Antioxidant therapy must be improving perhaps using combination between antioxidants.


Reproduction, Fertility and Development | 2017

135 NEW INSIGHTS ON THE ROLE OF 17β-ESTRADIOL IN CORPUS LUTEUM LIFESPAN OF NON-PREGNANT BITCHES

A.P.M. Cardoso; R. O. C. Silva; F. Garcia; Ines Cristina Giometti; Paula de Carvalho Papa

Canine corpus luteum (CL) is a transient endocrine gland responsible for the synthesis of 17β-oestradiol (E2) during diestrus and acts in an autocrine and/or paracrine manner within this structure. The mechanism of action of E2 depends on the expression ratio of its receptors ERa and ERβ. Binding to ERa has a proliferative effect and to ERβ an antiproliferative effect. The aim of this study was to better understand the possible signalling pathways mediated by ESR1 and ESR2 in the formation and regression of canine corpus luteum. The CL were collected via ovariosalpingohysterectomy from nonpregnant bitches (n=30) on Days 10, 20, 30, 40, 50, and 60 (n=5/group) post-ovulation. Eighteen CL (n=3/group) were subjected to RNA sequencing (RNA-Seq) to identify differentially expressed (DE) genes during diestrus because 3 replicates per group is the minimum number required to obtain good results in the sequencing. The DE genes were submitted to oPOSSUM 3 software (http://opossum.cisreg.ca/oPOSSUM3/) for detection of over-represented conserved transcription binding sites (TFBS) related to ESR1 and ESR2 (coding genes for ERa and ERβ, respectively). We validated the expression from 10 of these genes by RT-PCR using GAPDH as the reference gene. Protein expression of the IGF-related genes was also evaluated by immunohistochemistry. The RNAseq results were analysed by Cufflinks. Data were tested for homogeneity and normality using the Kolmogorov-Smirnov test. The RT-PCR data were compared by the one-way ANOVA test. The correlations between the RNAseq and RT-PCR results were verified by Pearson correlation. The difference was considered significant when P<0.05. All statistical analyses were performed with GraphPadPrism 5 (GraphPad Software Inc., San Diego, CA, USA). Differential gene expression analysis among groups during the luteal phase showed the presence of 5116 DE genes in at least one comparison, and 1106 genes that have not been recorded to the canine genome yet. Among all DE genes, we found 295 genes showing TFBS related to ESR1 and ESR2. Of these genes, 4 that had TFBS in common with ESR2 (LEF-1, PAPPA, NDGR2, and ATP1A1) and 1 with ESR1 (CAV1) were selected for validation, and the other 5 genes were chosen because they control cell proliferation (CTNNB1, CCND1, IGFBP3, IGFBP4, and IGFBP5). The selected genes belonged to IGF system-related genes (PAPPA, IGFBP3, IGFBP4, and IGFBP5), Wnt/betacatenin signalling (CTNNB1, LEF-1, and CCND1), and genes regulated by oestrogen hormones (NDRG2 and ATP1A1) and plasma membrane ER (CAV1). The results suggest that during the first half of diestrus, E2 signalling appears to be mediated by ERa via interaction with caveolin-1 (non-genomic pathways): IGF system and Wnt/β-catenin signalling were identified as one of the cascades activated by this interaction, with a major role in the proliferative process. During the second half of diestrus, ERβ appears to regulate NDGR2 and ATP1A1 gene expression, contributing to the regression of the CL. Therefore, the results suggest that E2 might activate both luteotrophic and regression-related factors in canine CL.


Reproduction, Fertility and Development | 2011

75 INFLUENCE OF CRYOPRESERVATION ON THE SUSCEPTIBILITY OF GOAT SPERM AGAINST DIFFERENT REACTIVE OXYGEN SPECIES

P. A. A. Góes; M. Nichi; R. O. C. Silva; E. G. A. Perez; A. Dalmazzo; J. R. C. Gurgel; C. C. Rocha; R. Simões; M. A. Peres; M. E. O. A. Assumpção; Renato Campanarut Barnabe; Valquiria Hyppolito Barnabe


Reproduction, Fertility and Development | 2011

91 TREATMENT OF GOAT SPERM WITH CATALASE TO IMPROVE POST-THAW QUALITY

R. O. C. Silva; M. Nichi; E. G. A. Perez; P. A. A. Góes; A. Dalmazzo; J. R. C. Gurgel; C. C. Rocha; R. Simões; M. A. Peres; M. E. O. A. Assumpção; Renato Campanarut Barnabe; Valquiria Hyppolito Barnabe


Reproduction, Fertility and Development | 2011

235 MANGALARGA STALLION SPERM IS HIGHLY SUSCEPTIBLE TO THE HYDROXYL RADICAL

J. R. C. Gurgel; M. Nichi; E. G. A. Perez; P. A. A. Góes; A. Dalmazzo; R. O. C. Silva; C. C. Rocha; R. Simões; M. A. Peres; M. E. O. A. Assumpção; V. H. Barnabe; Renato Campanarut Barnabe


Reproduction, Fertility and Development | 2011

240 FUNCTIONAL TRAITS OF CAT SPERM DURING DISTINCT MATURATION STATUS

E. G. A. Perez; M. Nichi; C. A. Baptista Sobrinho; P. A. A. Góes; A. Dalmazzo; J. R. Gurgel; C. C. Rocha; R. O. C. Silva; Renato Campanarut Barnabe; Valquiria Hyppolito Barnabe


Reproduction, Fertility and Development | 2011

232 INFLUENCE OF SEMINAL PLASMA ON THE SUSCEPTIBILITY OF DOG SPERM AGAINST DIFFERENT REACTIVE OXYGEN SPECIES

A. Dalmazzo; P. A. A. Góes; M. Nichi; R. O. C. Silva; J. R. C. Gurgel; E. G. A. Perez; C. C. Rocha; R. Simões; M. A. Peres; M. E. O. A. Assumpção; Renato Campanarut Barnabe; V. H. Barnabe


Reproduction, Fertility and Development | 2010

317 RESISTANCE AGAINST DIFFERENT REACTIVE OXYGEN SPECIES IN BOVINE EPIDIDYMAL SPERM UNDER DISTINCT MATURATION STATUS

M. Nichi; E. G. A. Perez; C. H. C. Viana; A. C. Teodoro; P. A. A. Góes; P. E. J. Bols; A. Dalmazzo; G. H. Ferreira; R. O. C. Silva; Renato Campanarut Barnabe; Valquiria Hyppolito Barnabe


Reproduction, Fertility and Development | 2010

34 OVINE SPERM IS HIGHLY SUSCEPTIBLE TO ATTACK BY HYDROGEN PEROXIDE

E. G. A. Perez; M. Nichi; F. A. Oliveira Neto; R. O. C. Silva; A. Dalmazzo; C. H. C. Viana; D. M. C. Pesce; P. A. A. Góes; G. H. Ferreira; Valquiria Hyppolito Barnabe; Renato Campanarut Barnabe


Reproduction, Fertility and Development | 2010

320 SUSCEPTIBILITY OF GOAT SPERM TO DIFFERENT REACTIVE OXYGEN SPECIES

R. O. C. Silva; E. G. A. Perez; R. P. Cabral; D. G. Silva; C. H. C. Viana; M. Nichi; P. A. A. Góes; G. H. Ferreira; A. C. Teodoro; Renato Campanarut Barnabe; Valquiria Hyppolito Barnabe

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E. G. A. Perez

University of São Paulo

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M. Nichi

University of São Paulo

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P. A. A. Góes

University of São Paulo

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A. Dalmazzo

University of São Paulo

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C. C. Rocha

University of São Paulo

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M. A. Peres

University of São Paulo

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