R. S. Pandey

Diurnal Variations of Oestradiol-17 β in Milk of Cross-Bred Cows During Oestrous Cycle and Early Pregnancy

SUMMARY Immunoreactive oestradiol-17β in milk was measured by radioimmunoassay in nine cross-bred dairy cows. The apparent oestradiol7β concentration was found to be significantly higher in evening (P

Diurnal variation of progesterone levels in milk and milk fat of crossbred cows during the oestrous cycle and early pregnancy.

Changes in progesterone levels in milk and in milk fat were measured by radioimmunoassay in nine crossbred cows for 40 days after insemination. On average, progesterone in milk was minimal: 1·10μg/l at the morning milking (M) and 1·63μg/l at the evening milking (E) on the day of oestrus; this increased to 15·72 μg/l at M and 22·8 μg/1 at E on day 13 after insemination, and thereafter declined to 0·99 μg/l at M and 1·12μg/l at E on the day that the next oestrous cycle commenced in non-pregnant cows. In pregnant animals the level was maintained, and fluctuated from 1505 to 19·60μg/l at M and 18·09 to 23·01 μ/1 at E up to 40 days of pregnancy. The average progesterone concentration in milk fat was 18·9μg/l at M and 24·1 μl at E on the day of oestrus, and rose to a peak of 141·7μ/l at M and 153·8μg/1 at E on day 13 after insemination. It continued to increase and fluctuated between 160·6 and 195·8μg/l at M, and 161·1 and 225·6μg/l at E, in pregnant animals, but dropped to 12·0μg/1 at M and 16·7 μg/l at E on the day of the succeeding oestrus in those animals which failed to conceive. Progesterone concentration in milk and in milk fat was significantly higher in evening samples than in morning samples (P

Hormonal changes in post-partum blood plasma and milk of buffaloes (Bubalus bubalis)

Post-partum changes in the concentration of progesterone, oestradiol-17β and prolactin were measured by radioimmunoassay in the blood plasma and milk of 28 buffaloes. The mean concentration of progesterone in the plasma of normal animals was 0·24 ± 0·04 μ.g/1 on the day of calving and decreased linearly during the post-partum period, until the complete regression of the residual corpus luteum of pregnancy occurred, when the plasma concentration was recorded as 0μ05 to 0μ08 μg/l. The concentration at a silent oestrus was 0μ17 ± 0·02 μ.g/1. The milk progesterone profile was parallel to that of plasma but the level was 2 to 4 times higher. The mean concentration of oestradiol-17β in plasma was 41·0 ± 2·34 ng/1 on the day of calving; it declined significantly by day 1 post partum ( P The mean concentration of prolactin in plasma was 212·6± 19·08 μg/1 on the day of calving and then declined and fluctuated between 70 and 270 μg/1 during the rest of the period. A minor elevation occurred around oestrus. The prolactin concentration for the first 2 days post partum in milk was significantly higher than that in plasma but during the rest of the period it was similar to that in plasma. The progesterone concentrations were slightly lower in anoestrous animals than in those cycling. The concentration of prolactin was significantly higher in multiparous animals ( P

Luteinizing hormone in blood plasma of post-partum buffaloes (Bubalus bubalis)

The changes in luteinizing hormone concentration were measured by heterologous double-antibody radioimmunoassay in blood plasma of 28 Murrah buffaloes. The LH concentration fluctuated between 0.22 to 0.78 ng/ml during first 21 days post-partum. The level increased significantly (P < 0.001) at estrus. The basal LH concentration of second and third week post-partum was inversely related to the first post-partum ovulation interval.

Gonadal steroid hormone level in blood plasma and milk of primiparous and multiparous nonpregnant and pregnant buffaloes

Changes in the concentration of progesterone and estradiol-17beta were measured by radioimmunoassay in 11 primiparous and 17 multiparous buffaloes at estrus and daily post insemination and in 6 nonbred buffaloes at 6 hour intervals from 4 days before expected estrus to one day after estrus. Plasma progesterone concentration at estrus was 0.1 ng/ml which rose to a peak level of 3.47 ng/ml on day 17. It fluctuated around this level in those animals which conceived, but followed a declining trend in those which failed to do so and attained lowest values on the day of next estrus. Temporal changes of the hormone revealed that the occurrence of major decline varied between 16 and 62 h before estrus. The average concentration in milk was about three to four times higher than in plasma. The concentration of estradiol-17beta about 23.50 pg/ml at estrus and fluctuated around 10 pg/ml in animals that returned to estrus with a peak around estrus. Temporal changes of hormone revealed that peak level occurred 8-17 h before estrus. The concentration of estradiol in pregnant animals fluctuated around 10 pg/ml. The concentration in milk was about 2-3 times higher than in plasma. There was no significant (P>0.05) difference in the concentrations of progesterone and estradiol-17beta between primiparous and multiparous animals.

Milk progesterone levels to monitor reproductive status of Murrah buffalo

Concentration of progesterone in whole milk was used to diagnose pregnancy in 44 lactating buffaloes. Milk samples were taken from days 19 to 27 post breeding and analysed for progesterone by radio-immunoassay. A level exceeding 10 ng/ml of milk was taken as an indication of pregnancy. Using this criterion, the accuracy of the pregnancy test from a single milk sample on 19, 21, 23, 25 and 27 days after insemination ranged from 71.42 to 86.95% and 86 to 87.50% for pregnant and non-pregnant animals, respectively. Milk progesterone concentrations were significantly higher (P / 0.001) in pregnant compared with those in non-pregnant buffaloes on day 19, 21, 23, 25 and 27 post-insemination.