R. Weiger

The ability of root ZX apex locator to reduce the frequency of overestimated radiographic working length.

The aim of this in vitro study was to evaluate the ability of the Root ZX device to avoid instrumentation beyond the apical foramen in premolars after conventional working length radiography. Thirty extracted premolars with 43 root canals were subjected to radiographic and electronic working length determination. Radiographic working length determination resulted in overestimation in 51% of the root canals, although the measuring file tip was located to be 0 to 2 mm short of the radiographic apex. Electronic working length measurements with the Root ZX reduced the percentage of overestimation to 21%. In 6 root canals (14%), both radiographic and electronic working length measurements led to overestimation. It is concluded that complementing radiographic working length determination with electronic apex locator measurements may help to avoid overestimation beyond the apical foramen in premolars.

Frequency of Overinstrumentation with an Acceptable Radiographic Working Length

The aim of this in vitro study was to determine how frequent a seemingly accurate working length ending radiographically 0 to 2 mm short of the radiographic apex resulted in an instrumentation beyond the apical foramen. Under simulated clinical conditions working lengths of 169 root canals were radiographically determined in 91 extracted teeth. In all cases the measuring files adjusted to the final working length (Iwork) were located 0 to 2 mm short of the radiographic apex. Iwork was subsequently compared with the actual reference length (Iref) representing the distance between the apical foramen and the coronal reference. Instrumentation beyond the apical foramen (Iwork > ref) occurred in premolars in 51% (95% confidence interval: 36%; 66%) of the cases, in molars in 22% (95% confidence interval: 14%; 30%), and in anterior teeth in no case. These results suggest that in premolars and molars a radiographically working length ending 0 to 2 mm short of the radiographic apex provides, more often than expected, a basis for unintentional overinstrumentation.

DEPOSITION AND RETENTION OF VITAL AND DEAD STREPTOCOCCUS SANGUINIS CELLS ON GLASS SURFACES IN A FLOW-CHAMBER SYSTEM

The proportion of vital as compared with dead Streptococcus sanguinis cells attached to glass surfaces was monitored and related to varying proportions of planktonic vital as compared with dead Strep. sanguinis cells. In a flow chamber with six parallel-mounted glass plates, Strep. sanguinis was suspended in pretreated sterile human saliva. Deposition of Strep. sanguinis took place, with a proportion of vital sanguinis streptococci in saliva (%VSs) of 90%, 45% or 22.5%. After exposure times of 30, 60, 90, 120 and 240 min, adherent microorganisms were labelled with two fluorescence stains to differentiate between vital and dead bacteria. Proportions of vital attached streptococci (%VSa) were determined microscopically. Dead bacteria were detected on all glass plates. The %VSa at 30 min and 60 min was significantly lower than the baseline %VSs. During the course of a single run the %VSa frequently increased after either 30, 60 or 90 min without exceeding the %VSs at 4 h. %VSs was the only variable exerting a significant effect on %VSa at 30 and 60 min. It is suggested that during the initial events of microbial attachment the dead rather than vital Strep. sanguinis cells attach preferably to solid surfaces.

An approach to differentiate between antibacterial and antiadhesive effects of mouthrinses in vivo

An experimental set-up allowing differentiation in vivo between antibacterial and antiadhesive properties of mouthrinses is described. The percentage of vital bacteria (= microbial vitality) and the bacterial counts were microscopically evaluated in saliva and in supragingival dental plaque both collected simultaneously at various times during de novo plaque formation. In a cross-over design, 12 healthy participants refrained from all oral hygiene for four separate periods of 2 x 4 h and 2 x 72 h after having rinsed with either an amine fluoride/stannous fluoride solution (Meridol) or 0.9% NaCl (placebo). Stimulated whole saliva was collected before and after the rinse. Together with whole-saliva samples, representative 4, 24 and 72-h-old plaque samples were separately taken from defined vestibular tooth surfaces that had been either exposed to the mouthrinse (unprotected sites) or temporarily covered with inert plastic films (protected sites) during rinsing. The pooled plaque and saliva were stained with fluorescent dyes to differentiate vital from dead micro-organisms which permitted the estimation of the percentages of vital bacteria. The total bacterial counts were quantified under the darkfield microscope. The Wilcoxon test was used for selected pairwise comparisons (alpha = 0.05). The percentage of vital bacteria in saliva fell significantly from 80-95% to about 50-60% as a result of the antibacterial activity of the test solution. These baseline values and those found in the presence of 4 and 24-h-old plaque were frequently lower than those recorded after the placebo rinse. In comparison to the placebo, microbial vitality was significantly reduced in early supragingival plaque formed on unprotected sites after applying the test solution. The similar total bacterial counts in 4-h-old plaque recorded after the use of the test solution on the unprotected and the protected areas did not point to an antiadhesive effect of the agent. It is concluded that this new experimental set-up allows decoding of the mode of action of a mouthrinse.