Radu G. Cucu

En-face optical coherence tomography - a novel application of non-invasive imaging to art conservation

Optical Coherence Tomography (OCT) is an optical interferometric technique developed mainly for in vivo imaging of the eye and biological tissues. In this paper, we demonstrate the potential of OCT for non-invasive examination of museum paintings. Two en-face scanning OCT systems operating at 850 nm and 1300 nm were used to produce B-scan and C-scan images at typical working distances of 2 cm. The 3D images produced by the OCT systems show not only the structure of the varnish layer but also the paint layers and underdrawings (preparatory drawings under the paint layers). The highest ever resolution and dynamic range images of underdrawings are presented and for the first time it is possible to find out non-invasively on which layer the underdrawings were drawn.

Combined multiplanar optical coherence tomography and confocal scanning ophthalmoscopy

We demonstrate the clinical application of a multiplanar imaging system that simultaneously acquires en face (C-scan) optical coherence tomography (OCT) and the corresponding confocal ophthalmoscopic images, along with cross-sectional (B-scan) OCT at specifiable locations on the confocal image. The advantages of the simultaneous OCT and confocal acquisition as well as the challenges of interpreting the C-scan OCT images are discussed. Variations in tissue inclination with respect to the coherence wave surface alter the sampling of structures within the depth of the retina, producing novel slice orientations that are often challenging to interpret. We have evaluated for the first time the utility of C-scan OCT for a variety of pathologies, including melanocytoma, diabetic retinopathy, choroidal neovascular membrane, and macular pucker. Several remarkable new aspects of clinical anatomy were revealed using this new technique. The versatility of selective capture of C-scan OCT images and B-scan OCT images at precise points on the confocal image affords the clinician a more complete and interactive tool for 3-D imaging of retinal pathology.

Sequential optical coherence tomography and confocal imaging

We report a system capable of sequentially acquiring two en-face images of different depth resolutions. The two images are generated by use of different principles, optical coherence tomography (OCT) and confocal microscopy, and have depth resolutions, at present, of better than 20 microm and over 0.12 mm, respectively. The lower-depth-resolution image is ideal for target positioning before collection of stacks of en-face OCT images. Switching between the two types of image by flipping an opaque screen in the reference arm, coupled with self-adjusting gain operation of avalanche photodiodes in the receiver. We illustrate the usefulness of the system by imaging a leaf and an optic nerve in vivo.

Combined confocal/en face T-scan-based ultrahigh-resolution optical coherence tomography in vivo retinal imaging

Combined confocal scanning ophthalmoscopy/en face T-scan-based ultrahigh-resolution optical coherence tomography (OCT) of the human retina in vivo is reported for the first time to our knowledge. The system uses a superluminescent diode-based broadband source, which gives an axial resolution of 3.2 microm in the retina. We demonstrate acquisition of T-scan-based OCT B-scan and simultaneous confocal/C-scan images of the human retina of large lateral size (covering a field of up to 20 degrees ) at a frame rate of 2Hz.

Simultaneous low coherence interferometry imaging at two depths using an integrated optic modulator

Abstract A Mach–Zehnder unbalanced LiNbO3 integrated modulator with independent control of the phase of each arm is incorporated into the reference arm of a low coherence interferometer set-up. Using different RF modulation frequency and processing electronics tuned to these frequencies, the system can be used for simultaneous interrogation of the signal reflected from two different depths in tissue or from two different axial positions in profilometry. When a pair of XY scanning mirrors are incorporated into the sensing arm, then two en-face images from different axial positions can be simultaneously produced. The depth separation between the axial positions of the points or layers interrogated is equal to half of the modulator path difference. The operation of the system is illustrated by displaying simultaneously two images from a coin.

Optical coherence tomography in otolaryngology: Original results and review of the literature

Optical coherence tomography is a diagnostic imaging technique allowing two dimensional tomographic imaging of tissue architecture. This is a review article on the use of optical coherence tomography in Otolaryngology including original images from human laryngeal tissue and temporal bones (cochlea) in our laboratory. Tissue specimens from normal larynges were imaged with an 850 nm OCT system. Our results showed good correlation between OCT image s and the corresponding haematoxylin-eosin stained histology sections in the normal larynx. Human temporal bones were also imaged using an 1300 nm OCT system. Limited morphological details were obtained due to the high scattering properties of the bony labyrinth.

Ultrahigh-Resolution Combined Coronal Optical Coherence Tomography Confocal Scanning Ophthalmoscope (OCT/SLO): A pilot study

SummaryOBJECTIVE: To evaluate clinical images from a prototype ultrahigh resolution (UHR) combined coronal optical coherence tomography/confocal scanning ophthalmoscope (OCT/SLO) and to compare them to standard-resolution OCT/SLO images on the same patients. DESIGN: Cross-sectional pilot-study. PARTICIPANTS: Sixty-six eyes of 42 patients with various macular pathologies, such as age-related macular degeneration, macular edema, macular hole, central serous retinopathy, epiretinal membrane and posterior vitreous traction syndrome. METHODS: Each subject was first scanned with a standard-resolution OCT/SLO that has an axial resolution of ∼10 micron. Immediately following, patients were scanned with the prototype UHR OCT/SLO device. The UHR system employs a compact super luminescent diode (SLD) with a 150 nm bandwidth centered at 890 nm, which allows imaging of the retina with an axial resolution of 3 microns. Both coronal and longitudinal OCT scans were acquired with each system, and compared side-by-side. Scan quality was assessed for the observers ability to visualize the vitreo-retinal interface and retinal layers – in particular of the outer retina/RPE/choroidal interface, increased discrimination of pathological changes, and better signal intensity. MAIN OUTCOME MEASURES: Ultrahigh and standard-resolution coronal and longitudinal OCT/SLO images of macular pathologies. RESULTS: In the side-by-side comparison with the commercial standard-resolution OCT/SLO images, the scans in the Ultrahigh resolution OCT/SLO images were superior in 85% of cases. Relatively poor quality images were attributed to lower signal-to-noise ratio, limited focusing, or media opacities. Several images that had a better signal intensity in the standard-resolution OCT/SLO system were found to show more retinal detail in the UHR system. In general, intraretinal layers in the UHR OCT/SLO images were better delineated in both coronal and longitudinal scans. Enhanced details were also seen in the outer retina/RPE/choroidal complex. The UHR OCT/SLO system produced better definition of morphological changes in several macular pathologies. CONCLUSIONS: Broadband SLD-based UHR OCT/SLO offers a compact, efficient, and economic enhancement to the currently available clinical OCT imaging systems. UHR OCT/SLO imaging enhanced the quality of the OCT C-scans, facilitated appreciation of vitreo-retinal pathologies, and improved sensitivity to small changes in the retina, and the outer retina/RPE/choroidal interface.

Quasi-simultaneous OCT en-face imaging with two different depth resolutions

We report a system capable of acquiring two quasi-simultaneous en-face optical coherence tomography (OCT) images of different depth resolution (one better than 20 μm and the other between 80 and 330 μm) at a frame rate of 2 Hz. The larger depth resolution image makes it ideal for target positioning in the OCT imaging of moving organs, such as eye fundus and cornea, as well as in the alignment of stacks of en-face OCT images. This role is similar to that of the confocal channel in a previously reported dual channel OCT/confocal imaging instrument. The system presented operates as a dual channel imaging instrument, where both channels operate on the OCT principle. We illustrate the functionality of the system with examples from a coin, skin from a finger and optic nerve in vivo.

Polarization-sensitive OCT system using single-mode fiber

Polarization sensitive optical coherence tomography (PS-OCT) takes into account the vector nature of light waves (state of polarization). The most complete information about the polarization properties of a biological target is given by depth resolved Mueller matrix elements. However, the construction of a Mueller matrix PS-OCT easy to use for in-vivo imaging is a difficult task. We designed and assembled a simpler and more versatile system that provides only essential depth-resolved information about the tissue polarization properties (linear birefringence retardation). The interferometer is a hybrid configuration of bulk optic and single mode (SM) optical fiber components. No polarization maintaining (PM) fiber is used. The reference and sample beams interfere in two single mode fiber couplers. The system can display either a pair of OCT images corresponding to linear orthogonal polarization components or a pair of images consisting of a polarization insensitive (pure reflectivity) image and a linear birefringence retardation map. All images are 8 bit grayscale and are acquired by fast en face scanning (T-scan) at 2 frames/s. We demonstrate in vivo en face images of the optic nerve region and the peripheral cornea.

En face polarization sensitive optical coherence tomography

We report the first (to the best of out knowledge) en face polarization sensitive optical coherence tomography (PS-OCT) system. The transverse raster scanning of the target is achieved using a pair of galvo-scanner mirrors. The set-up is based on incoherent detection in two optical and electronic channels and employs balanced detection to reduce the excess photon noise generated by the low coherence source (superluminescent diode). The outputs of the two channels are processed using software to provide a polarisation insensitive (pure reflectivity) image and a birefringence retardation map. Images from ex vivo (human tooth) and in vivo targets (human retina) have been acquired. Particulars of en face optical coherence tomography imaging of birefringent tissue are discussed.