Raf Aerts

Towards domestication of Jatropha curcas

Jatropha curcas L. attracts a lot of interest as a biofuel crop, triggering large investments and rapid expansion of cultivation areas, and yet, it should still be considered as a (semi-)wild, undomesticated plant. To use the full potential of Jatropha and to support further expansion and systematic selection, breeding and domestication are a prerequisite. This review reveals and identifies gaps in knowledge that still impede domestication of Jatropha. Prebreeding knowledge is limited. In particular, the regeneration ecology and the degree of genetic diversity among and within natural populations in and outside the center of origin are poorly studied. There is only a limited understanding of the Jatropha breeding system and the effect of inbreeding and outbreeding. This review presents all currently available and relevant information on the species distribution, site requirements, regeneration ecology, genetic diversity, advances in selection, development of varieties and hybridization. It also describes possible routes to a better Jatropha germplasm, gives recommendations for tackling current problems and provides guidance for future research. We also discuss the participatory domestication strategy of Jatropha integration in agroforestry.

Forest restoration, biodiversity and ecosystem functioning.

Globally, forests cover nearly one third of the land area and they contain over 80% of terrestrial biodiversity. Both the extent and quality of forest habitat continue to decrease and the associated loss of biodiversity jeopardizes forest ecosystem functioning and the ability of forests to provide ecosystem services. In the light of the increasing population pressure, it is of major importance not only to conserve, but also to restore forest ecosystems.Ecological restoration has recently started to adopt insights from the biodiversity-ecosystem functioning (BEF) perspective. Central is the focus on restoring the relation between biodiversity and ecosystem functioning. Here we provide an overview of important considerations related to forest restoration that can be inferred from this BEF-perspective.Restoring multiple forest functions requires multiple species. It is highly unlikely that species-poor plantations, which may be optimal for above-ground biomass production, will outperform species diverse assemblages for a combination of functions, including overall carbon storage and control over water and nutrient flows. Restoring stable forest functions also requires multiple species. In particular in the light of global climatic change scenarios, which predict more frequent extreme disturbances and climatic events, it is important to incorporate insights from the relation between biodiversity and stability of ecosystem functioning into forest restoration projects. Rather than focussing on species per se, focussing on functional diversity of tree species assemblages seems appropriate when selecting tree species for restoration. Finally, also plant genetic diversity and above - below-ground linkages should be considered during the restoration process, as these likely have prominent but until now poorly understood effects at the level of the ecosystem.The BEF-approach provides a useful framework to evaluate forest restoration in an ecosystem functioning context, but it also highlights that much remains to be understood, especially regarding the relation between forest functioning on the one side and genetic diversity and above-ground-below-ground species associations on the other. The strong emphasis of the BEF-approach on functional rather than taxonomic diversity may also be the beginning of a paradigm shift in restoration ecology, increasing the tolerance towards allochthonous species.

Global mapping of Jatropha curcas yield based on response of fitness to present and future climate.

Although acclaimed as a biofuel crop with high potential to sustainably replace fossil fuels, Jatropha curcas L. remains a poorly studied plant. Reliable yield assessments with conventional methods require agroclimatic and physiological knowledge, which is not yet available for Jatropha. To fill this gap, we tested a novel two‐step approach integrating knowledge from biogeography and population biology with available Jatropha field data. In the first step, using MaxEnt, a widely implemented model in biogeography, we predicted Jatropha fitness in response to climate by relating natural occurrence recorded in herbaria with bioclimatic geodatasets. In the second step, we relied on population biology principles supported by seed mass addition experiments to relate fitness to reproductive potential, hence seed yield. Jatropha seed yield in response to climate was mapped worldwide for actual (1950–2000 average) and future (2020) climate conditions. The modelled Jatropha seed yield was validated against a set of on‐field yield assessments (R2=0.67, P<0.001). The discrepancies between estimated and measured yields were partially explained by model uncertainties, as quantified by the sensitivity analysis of our modelling (R2=0.57, P=0.001). Jatropha has a pan‐tropical distribution, plus specific adaptability to hot temperate areas. Climate variables most significantly affecting modelled yield response were annual average temperature, minimum temperature, annual precipitation and precipitation seasonality.

Genetic variation and risks of introgression in the wild Coffea arabica gene pool in south-western Ethiopian montane rainforests

The montane rainforests of SW Ethiopia are the primary centre of diversity of Coffea arabica and the origin of all Arabica coffee cultivated worldwide. This wild gene pool is potentially threatened by forest fragmentation and degradation, and by introgressive hybridization with locally improved coffee varieties. We genotyped 703 coffee shrubs from unmanaged and managed coffee populations, using 24 microsatellite loci. Additionally, we genotyped 90 individuals representing 23 Ethiopian cultivars resistant to coffee berry disease (CBD). We determined population genetic diversity, genetic structure, and admixture of cultivar alleles in the in situ gene pool. We found strong genetic differentiation between managed and unmanaged coffee populations, but without significant differences in within‐population genetic diversity. The widespread planting of coffee seedlings including CBD‐resistant cultivars most likely offsets losses of genetic variation attributable to genetic drift and inbreeding. Mixing cultivars with original coffee genotypes, however, leaves ample opportunity for hybridization and replacement of the original coffee gene pool, which already shows signs of admixture. In situ conservation of the wild gene pool of C. arabica must therefore focus on limiting coffee production in the remaining wild populations, as intensification threatens the genetic integrity of the gene pool by exposing wild genotypes to cultivars.

Effects of coffee management intensity on composition, structure and regeneration status of Ethiopian moist evergreen Afromontane forests

The effect of arabica coffee management intensity on composition, structure, and regeneration of moist evergreen Afromontane forests was studied in three traditional coffee-management systems of southwest Ethiopia: semiplantation coffee, semiforest coffee, and forest coffee. Vegetation and environmental data were collected in 84 plots from forests varying in intensity of coffee management. After controlling for environmental variation (altitude, aspect, slope, soil nutrient availability, and soil depth), differences in woody species composition, forest structure, and regeneration potential among management systems were compared using one way analysis of variance. The study showed that intensification of forest coffee cultivation to maximize coffee production negatively affects diversity and structure of Ethiopian moist evergreen Afromontane forests. Intensification of coffee productivity starts with the conversion of forest coffee to semiforest coffee, which has significant negative effects on tree seedling abundance. Further intensification leads to the conversion of semiforest to semiplantation coffee, causing significant diversity losses and the collapse of forest structure (decrease of stem density, basal area, crown closure, crown cover, and dominant tree height). Our study underlines the need for shade certification schemes to include variables other than canopy cover and that the loss of species diversity in intensively managed coffee systems may jeopardize the sustainability of coffee production itself through the decrease of ecosystem resilience and disruption of ecosystem services related to coffee yield, such as pollination and pest control.

Global greenhouse gas implications of land conversion to biofuel crop cultivation in arid and semi-arid lands: lessons learned from Jatropha

Biofuels are considered as a climate-friendly energy alternative. However, their environmental sustainability is increasingly debated because of land competition with food production, negative carbon balances and impacts on biodiversity. Arid and semi-arid lands have been proposed as a more sustainable alternative without such impacts. In that context this paper evaluates the carbon balance of potential land conversion to Jatropha cultivation, biofuel production and use in arid and semi-arid areas. This evaluation includes the calculation of carbon debt created by these land conversions and calculation of the minimum Jatropha yield necessary to repay the respective carbon debts within 15 or 30 years. The carbon debts caused by conversion of arid and semi-arid lands to Jatropha vary largely as a function of the biomass carbon stocks of the land use types in these regions. Based on global ecosystem carbon mapping, cultivated lands and marginal areas (sparse shrubs, herbaceous and bare areas) show to have similar biomass carbon stocks (on average 4e 8tCh a � 1 ) and together cover a total of 1.79 billion ha.

Effects of pioneer shrubs on the recruitment of the fleshy-fruited tree Olea europaea ssp. cuspidata in Afromontane savanna

Abstract Question: Is seedling recruitment of a fleshy-fruited tree in degraded Afromontane savanna dependent on shelter from pioneer shrubs, and is shelter availability related to shrub traits? Location: Degraded montane savanna in northern Ethiopia (13°36′ N, 39°21′ E). Method: Nurse plants of Olea europaea ssp. cuspidata seedlings were recorded using T-square plotless sampling and clustered according to shrub traits, using Wards method after Principal Components Analysis. Facilitation was further examined through experimental planting and Kaplan-Meier survival analysis. Results: Both in grazed and protected areas, Olea recruits were found exclusively under shrubs, primarily under Euclea racemosa although Acacia etbaica was more abundant. Olea recruitment is distributed randomly at landscape scale, but depends on shelter at patch scale. Shelter ability is related to shrub shape and species identity. Dense multi-stemmed shrubs with a wide base and crown on a mulch-rich mound are key recruitment foci. Euclea shrubs have these favoured traits and probably act as preferential perching sites for avian seed dispersers. Soil and organic matter accumulation under Euclea shrubs may also create favourable conditions for Olea germination and survival. Experimentally planted seedlings had a better chance for survival under Euclea. Conclusions: Olea regeneration is probably subject to both passive (disperser-mediated) and active facilitation. Small changes of shrub traits can alter the suitability of a patch for Olea recruitment. Protection of shrubs can increase facilitation for seedlings, while pruning may reduce competition for saplings and thus enhance forest succession. Planting of raised Olea seedlings under Euclea shrubs in years with a good rainy season may further assist forest restoration. Abbreviation: TSB = Byth robust T-square estimator. Nomenclature: Hedberg & Edwards (1989); Hedberg et al. (2003); Klopper et al. (2005).

Invasiveness risk of the tropical biofuel crop Jatropha curcas L. into adjacent land use systems: from the rumors to the experimental facts

Jatropha curcas L. produces seeds rich in non‐edible oil suitable for biodiesel but it has been categorized as invasive. Although not scientifically verified, this allegation has resulted in a cultivation ban in several countries. In this article we report an integrated series of observations and experimental findings from invasiveness research in Zambia. We studied the impacts of J. curcas plantations on adjacent land use systems focusing on spontaneous occurrence of seedlings, seed dispersal mechanisms, seed predation by animals, and germination success of dispersed seeds. No spontaneous regeneration was observed in land use systems adjacent to J. curcas plantations. Primary seed dispersal was limited, predominantly under the canopy of the mother plant. Rodents and shrews dispersed and predated J. curcas seeds and fruits. They transported the seeds up to 23 m from the sources and repositioned them in their burrows up to 0.7 m deep, but none of these seeds could establish. Germination experiments in adjacent land use systems revealed 4% germination success at the soil surface, and 65% if buried artificially at 1–2 cm depth, yet the latter is unlikely to occur under natural conditions. These findings show that J. curcas seeds may be dispersed by animals to adjacent land use systems, but no natural recruitment was observed given low germination on the surface and none in burrows. Altogether these results suggest that the plant currently does not show an elevated risk of invasion to adjacent land use systems, at least in the investigated case study. But more long‐term studies, also in other growing areas are needed to corroborate these results.

Protecting coffee from intensification.

![Figure][1] Coffee beans PHOTO: TONY OQUIAS/[ISTOCKPHOTO.COM][2] Sustainable agricultural intensification is one approach to meet food security and biodiversity conservation goals simultaneously ([ 1 ][3], [ 2 ][4]). The yield increases required to achieve these goals can be facilitated by

Status of Native Woody Species Diversity and Soil Characteristics in an Exclosure and in Plantations of Eucalyptus globulus and Cupressus lusitanica in Northern Ethiopia

Abstract Exclusion of grazing animals and tree plantations are 2 methods that have been used for restoration of degraded lands in tropical semiarid areas. These 2 options can foster secondary forest succession by improving soil conditions, attracting seed-dispersal agents, and modifying microclimate for understory growth. We compared native woody plant diversity and soil chemical and physical attributes under exclosure and on Eucalyptus globulus (EGP) and Cupressus lusitanica (CLP) plantations. Vegetation data were collected by an inventory of stands with circular plots of 5.64 m radius, and soil samples were collected from the 4 corners and center of 20 × 20 m plots. As a result, 15 native woody plant species belonging to 13 families were recorded. Importance value index (IVI), Shannon-Wiener, Simpsons diversity, and species richness were higher in the exclosure, followed by EGP and CLP. Contents of soil organic carbon and total nitrogen showed significance difference and were highest in the exclosure, followed by CLP, EGP, and grazing land. Although the difference was not significant, an increasing trend was observed for cation exchange capacity and K+. Bulk density was highest on the grazing land, followed by EGP, CLP, and exclosure. Our results suggest that degraded land reclamation can be achieved with plantation of rapidly growing tree species as well as exclosure. However, native woody species diversity and improved soil attributes are better achieved with exclosure. Exclosures can be established interspersed strategically with single- or mixed-species plantations to facilitate restoration of native vegetation. However, it is important to conduct further research on the comparative advantage of enrichment planting of exclosures with preferred tree species or exclosures interspersed within blocks of plantation.