Rafael Boluda

Heavy metals incidence in the application of inorganic fertilizers and pesticides to rice farming soils

The concentrations of Cd, Co, Cu, Ni, Pb, Zn, Fe and Mn in different inorganic fertilizers (urea, calcium superphosphate, iron sulphate and copper sulphate) and in pesticides (two herbicides and one fungicide) are evaluated together with the contribution of these metals in soils from their use. The study was made in rice farming areas to the north of Albufera Natural Park (Valencia, Spain). The results obtained show that superphosphate is the fertilizer that contains the highest concentrations of Cd, Co, Cu and Zn as impurities. Copper sulphate and iron sulphate have the most significant concentrations of Pb, and are the only fertilizers in which Ni was detected. The three pesticides analysed show similar Cd contents and the highest levels of Fe, Mn, Zn, Pb and Ni are found in the herbicides. The most significant additions of heavy metals as impurities that soil receives from agricultural practices, are Mn, Zn, Co and Pb. Three contamination indexes have been applied to provide a basis for comparison of potential heavy metal toxicity. These results denote the potential toxicity of heavy metals in the studied soils.

Composting rice straw with sewage sludge and compost effects on the soil-plant system.

Composting organic residue is an interesting alternative to recycling waste as the compost obtained may be used as organic fertilizer. This study aims to assess the composting process of rice straw and sewage sludge on a pilot-scale, to evaluate both the quality of the composts obtained and the effects of applying such compost on soil properties and plant development in pot experiments. Two piles, with shredded and non-shredded rice straw, were composted as static piles with passive aeration. Throughout the composting process, a number of parameters were determined, e.g. colour, temperature, moisture, pH, electrical conductivity, organic matter, C/N ratio, humification index, cation exchange capacity, chemical oxygen demand, and germination index. Moreover, sandy and clayey soils were amended with different doses of mature compost and strewed with barley in pot experiments. The results show that compost made from shredded rice straw reached the temperatures required to maximise product sanitisation, and that the parameters indicating compost maturity were all positive; however, the humification index and NH(4) content were more selective. Therefore, using compost-amended soils at a dose of 34 Mg ha(-1) for sandy soil, and of 11 Mg ha(-1) for clayey soil improves soil properties and the growth of Hordeum vulgare plants. Under there conditions, the only limiting factor of agronomic compost utilisation was the increased soil salinity.

Application of the Microtox® test and pollution indices to the study of water toxicity in the Albufera Natural Park (Valencia, Spain)

The toxic effects of waters collected from irrigation channels in a Mediterranean wetland (Albufera Natural Park, Valencia, Spain) were tested with the Microtox assay and compared with six pollution indices (PIs) defined from analytical parameters. Chemical oxygen demand (COD), biological oxygen demand (BOD), nutrients, heavy metals and pesticides were measured. The bioassay result (concentrations of the water sample (% V/V) that reduced light emission to 10%, 20% and 50%, EC10, EC20 and EC50, respectively (ECs)) was compared with the PIs. This comparison has demonstrated a general agreement between ECs and PIs, except in the case of irrigation channels affected by herbicides used in rice farming (molinate and thiobencarb). No pronounced inhibition was detected in the bioluminescence in relation to the eutrophic parameters in the irrigation waters for EC50 values, indicating that this parameter does not suffice to detect eutrophic waters. Data derived from irrigation water pollution and bioassay were assembled by multivariate statistical techniques (principal component analysis). These components were associated with various contamination sources.

Environmental cadmium, lead and nickel contamination: possible relationship between soil and vegetable content

SummaryThe cadmium, lead and nickel content of soils of four agricultural areas exposed to different degrees of environmental pollution and vegetables grown there were measured by Atomic Absorption Spectrophotometry (AAS). In order to ascertain the possible relationship between the heavy metal content of soils (total and extractable) and of vegetables grown in them, the correlation between the two was calculated. The highest correlation values between metal content of soil and vegetables are these corresponding to nickel: the total metal content in soils and leaves-stems are linear (p<0.01). The extractable metal content of soils and leaves-stems are linear (p<0.1). Therefore, soil seems to be the main contributor to nickel content of plants (leaves-stems). In the case of cadmium a statistically significant linear correlation (p<0.01) was observed between total metal content in soil and in leaves-stems and a negative one between extractable metal content of soils and that of roots-bulbs. For lead a negative correlation was found between extractable metal content of soils and leaves-stems. In any case, the lead content of soils is not the main factor that influences the lead content of vegetables. Anthropogenic and environmental factors play a more important role than the lead in soils in the lead content of vegetables.

Soil Moisture Effect on Thermal Infrared (8–13-μm) Emissivity

Thermal infrared (TIR) emissivities of soils with different textures were measured for several soil moisture (SM) contents under controlled conditions using the Box method and a high-precision multichannel TIR radiometer. The results showed a common increase of emissivity with SM at water contents lower than the field capacity. However, this dependence is negligible for higher water contents. The highest emissivity variations were observed in sandy soils, particularly in the 8-9-μm range due to water adhering to soil grains and decreasing the reflectance in the 8-9-μm quartz doublet region. Thus, in order to model the emissivity dependence on soil water content, different approaches were studied according to the a priori soil information. Soil-specific relationships were provided for each soil texture and different spectral bands between 8 and 13 μm, with determination coefficients up to 0.99, and standard estimation errors in emissivity lower than ± 0.014. When considering a general relationship for all soil types, standard estimation errors up to ±0.03 were obtained. However, if other soil properties (i.e., organic matter, quartz, and carbonate contents) were considered, along with soil water content, the general relationship predicted TIR emissivities with a standard estimation error of less than ±0.008. Furthermore, the study showed the possibility of retrieving SM from TIR emissivities with a standard estimation error of about ±0.08 m3 . m-3.

Determination of Pesticides in Soil Samples by Solid Phase Extraction Disks

SummaryA systematic study comparing the methodology and analytical results obtained in an investigation of seven pesticide residues (Molinate, Atrazine, Carbofuran, Pirimicarb, Prometryn, Malathion and Tetrachlorvinphos) in soil samples is reported. Solid-phase extraction (SPE) using glass columns and 47 mm disks of octyl and octadecyl-bonded silica was used in the pesticide analysis. The best extraction efficiency and clearest extracts are obtained with C8 disks. The analyses were carried out by capillary gas chromatography with nitrogen and phosphorus detection. Recovery experiments were performed at ppb levels in spiked soil samples. The average recoveries of the compounds were 53–77%. Detection limits are between 5 and 30 ng g−1 based on 5 g moist soil sample. The method was validated by comparing it with conventional liquid-liquid extraction.

Distribution of heavy metals in rice farming soils

The distribution of Cd, Co, Cu, Ni, Pb, Zn, Mn, and Fe in soils dedicated to rice crops have been studied. Total content and extractable fraction have been determined by acid digestion and extraction with EDTA, respectively. A method of selective sequential extraction (SSE) has been applied to determine the fraction of the metals bound to organic matter, soluble plus exchangeable, precipitated with carbonates, associated to Fe and Mn amorphous oxides, precipitated with sulfides, and the residual; all the elements show their most important fraction as precipitates, mainly as forms of sulfides and carbonates. The forms available by plants and bound to the organic matter are less represented, except for Cu and Ni.

Determination and assessment of mercury content in calcareous soils.

This paper provides the first available information on the determination of the total mercury content in different Mediterranean calcareous soils by thermal decomposition, amalgamation and atomic absorption spectrophotometry. Fifty-three samples from five soil use groups (natural, dry land, greenhouse, irrigated and rice farming soils) were analysed. The results show that the levels of mercury in these soils were 9.4-1585mugkg(-1). Soil organic matter and the zinc equivalent index have been related to Hg content in soils, suggesting that Cu, Ni, Zn and Hg are of a similar origin. The background level (BL), geochemical baseline concentrations (GBC) and the reference value (RV) were established from natural soils. Calculations are according to the log-normal distribution criterion. The BL, GBC and RV values obtained were 25.1, 9.8-64.3, and 64.3mugkg(-1), respectively. The BL was similar to the baseline concentration in soils obtained at Admiralty Bay (Antarctica) (24.6mugkg(-1)) and a Spanish peat bog (Galicia) (22mugkg(-1)). These data show that rice farming soils (gleyic-calcaric Fluvisols, Fluvaquents), irrigated soils (calcaric Fluvisols, Xerofluvents) and some greenhouse soils (cumulic Antrosols) presented much higher levels, indicating contamination. Hg accumulation in these soils was associated with local anthropogenic sources.

Optimization of a solid-phase extraction technique for the extraction of pesticides from soil samples

Progress has been made towards optimizing a rapid, accurate method for extracting pesticides from soil for routine analyses. The basis of the method is the formation of a supernatant by sonication of the soil with a water-acetone mixture. After removal from the soil particles, the pesticides are extracted by passing through a disk of octyl-bonded silica sorbent. To establish the suitable extraction conditions, several variables affecting the performance of the C8 disk, such as the influence of the use of centrifugation or filtration for the removal of supernatant and soil particles and the effectiveness of the prior sonication step, such as water proportion, water temperature and pH of the extractive solution, have been studied. Maximum yields were obtained by extraction with a mixture of water at 50°C and acetone (1:1), and centrifugation to remove the supernatant prior to the solid-phase extraction (SPE). In order to evaluate the matrix effect in SPE of the pesticides, recovery experiments were performed for soil samples with different physical and chemical characteristics.

Relation between reflectance of rice crop and indices of pollution by heavy metals in soils of albufera natural park (Valencia, Spain)

Abstract The aim of the research was to relate the spectral response of rice to heavy metal concentration in soil of Albufera Natural Park. The spectral observations were values of the crop reflectance obtained from the Landsat-5 Thematic Mapper NIR and Red hands expressed by the Normalized Difference Vegetation Index, NDVI. Soil samples of the surface 20 cm were analyzed for total and extractable heavy metal content. The strongest correlations between NDVI and heavy metal in soil were found between the available fractions of Ni, Pb, Cd, Cu and Zn and the toxicity index, Zn equivalent, defined by Zn Eq = (1× Zn ) + (2× Cu ) + (8× Ni ) where metal concentrations are in mg per kg of soil.