Network


Latest external collaboration on country level. Dive into details by clicking on the dots.

Hotspot


Dive into the research topics where Rafael Martínez-Sanz is active.

Publication


Featured researches published by Rafael Martínez-Sanz.


International Journal of Cardiology | 2013

Expression of FGF23/KLOTHO system in human vascular tissue.

Javier Donate-Correa; Carmen Mora-Fernández; Rafael Martínez-Sanz; Mercedes Muros-de-Fuentes; Horacio Pérez; Beatriz Meneses-Pérez; Violeta Cazaña-Pérez; Juan F. Navarro-González

BACKGROUND Fibroblast growth factor (FGF)-23 levels have been associated with impaired vasoreactivity, increased arterial stiffness, and cardiovascular morbi-mortality, whereas a protective function of KLOTHO against endothelial dysfunction has been reported. Since expression of the FGF23-KLOTHO system in human vascular tissue remains unproved, we aimed to study the expression of FGF23, FGF receptors (FGFR) and KLOTHO in human aorta. In addition, we analyzed the FGF23-KLOTHO expression in occlusive coronary thrombi. METHODS Thoracic aorta specimens from 44 patients underwent elective cardiac surgery, and thrombus material from 2 patients with acute coronary syndrome (ACS), were tested for FGF23-KLOTHO system expression. RESULTS Expression of KLOTHO (mean expression level 4.85 ± 5.43, arbitrary units) and two of the three cognate FGFR (FGFR-1 and -3) were detected and confirmed by RT-PCR, sequencing and qRT-PCR. KLOTHO expression was confirmed within occlusive coronary thrombi from patients with ACS. However, expression of FGF23 and FGFR4 was not observed. We also detected the aortic expression of membrane-anchored A Desintegrin and Metalloproteinases (ADAM)-17, the enzyme responsible for the shedding of KLOTHO from the cell surface, and the anti-inflammatory cytokine interleukin (IL)-10. Interestingly, in aortic samples there was a direct association between KLOTHO mRNA levels and those of ADAM-17 and IL-10 (r = 0.54, P<0.001; r = 0.51, P<0.01, respectively). CONCLUSIONS Human vascular tissue expresses members of the FGF23-KLOTHO system, indicating that it can be a direct target organ for FGF23. In addition, KLOTHO expression is also detected in occlusive coronary thrombi. These findings suggest a putative role of FGF23-KLOTHO axis in human vascular pathophysiology and cardiovascular disease.


Heart | 2014

Reduced Klotho is associated with the presence and severity of coronary artery disease

Juan F. Navarro-González; Javier Donate-Correa; Mercedes Muros de Fuentes; Horacio Pérez-Hernández; Rafael Martínez-Sanz; Carmen Mora-Fernández

Objective Klotho is involved in vascular health. We aimed to analyse in a cross-sectional study the relationship between Klotho and human coronary artery disease (CAD). Methods The study included 371 subjects who underwent coronary angiography and 70 patients who underwent elective cardiac surgery recruited between May 2008 and June 2009. The presence and severity (stenosis index) of CAD, cardiovascular risk factors, Klotho gene expression in the thoracic aorta, and serum soluble Klotho concentrations were evaluated. Results The soluble Klotho concentration was lower (p<0.001) in patients with significant CAD (n=233). The maximal stenosis observed in every epicardial artery and the stenosis severity index was significantly lower in patients within the higher soluble Klotho concentrations (p<0.0001). Multiple regression analysis showed that serum Klotho concentrations were inverse and significantly associated with CAD (adjusted R2=0.67, p<0.001). Multivariate logistic regression analysis showed that risk factors for significant CAD included age, diabetes, smoking and inflammation, whereas high serum Klotho values were associated with a lower risk for CAD. Lower mRNA expression level of Klotho was observed in 46 patients with significant CAD, as compared with subjects without CAD (p=0.01). Logistic regression analysis showed that high Klotho gene expression was independently associated with lower risk for CAD. Conclusions Patients with significant CAD present lower soluble concentrations of Klotho, as well as reduced levels of Klotho gene expression in the vascular wall. Reduced serum Klotho concentrations and decreased vascular Klotho gene expression were associated with the presence and severity of CAD independently of established cardiovascular risk factors.


Interactive Cardiovascular and Thoracic Surgery | 2012

The effect of intravenous and oral iron administration on perioperative anaemia and transfusion requirements in patients undergoing elective cardiac surgery: a randomized clinical trial

Pilar Garrido-Martín; Mohamed Ibrahim Nassar-Mansur; Ramiro de la Llana-Ducrós; Tirso Virgos-Aller; Patricia María Rodríguez Fortunez; Rosa Ávalos-Pinto; Alejandro Jiménez-Sosa; Rafael Martínez-Sanz

OBJECTIVES Anaemia is a frequent complication after cardiopulmonary bypass surgery. Iron therapy has been variably employed by medical centres over the years. In our study we test the clinical effectiveness of intravenous and oral iron supplementation in correcting anaemia, and its impact on blood transfusion requirements, in patients undergoing cardiopulmonary bypass surgery. METHODS A double-blind, randomized, placebo-controlled clinical trial with three parallel groups of patients. Group I (n = 54): intravenous iron(III)-hydroxide sucrose complex, three doses of 100 mg/24 h during pre- and postoperative hospitalization and 1 pill/24 h of oral placebo in the same period and during 1 month after discharge. Group II (n = 53): oral ferrous fumarate iron 1 pill/24 h pre- and postoperatively and during 1 month after discharge, and intravenous placebo while hospitalized. Group III (n = 52): oral and intravenous placebo pre- and postoperatively, following the same protocol. Data were collected preoperatively, at theatre, at intensive care unit admission, before hospital discharge and 1 month later. RESULTS (1) Baseline clinical and demographic characteristics and surgical procedures were similar in the three groups; (2) no inter-group differences were found in haemoglobin and haematocrit during the postoperative period; (3) the intravenous iron group showed higher serum ferritin levels at hospital discharge (1321 ± 495 ng/ml; P < 0.001) and 1 month later (610 ± 387; P < 0.001) compared with the other groups and (4) we did not observe statistical differences in blood transfusion requirements between the three groups. CONCLUSIONS The use of intravenous or oral iron supplementation proved ineffective in correcting anaemia after cardiopulmonary bypass and did not reduce blood transfusion requirements. [Current Controlled Trials number: NCT01078818 (oral and intravenous iron in patients postoperative cardiovascular surgery under EC)].


Journal of Cellular and Molecular Medicine | 2016

Influence of Klotho gene polymorphisms on vascular gene expression and its relationship to cardiovascular disease.

Javier Donate-Correa; Ernesto Martín-Núñez; Rafael Martínez-Sanz; Mercedes Muros-de-Fuentes; Carmen Mora-Fernández; Nayra Pérez-Delgado; Juan F. Navarro-González

Klotho protein has been associated with beneficial effects that contribute to the maintenance of cardiovascular health. Diverse studies suggest that alterations in the levels of this molecule may be associated with pathophysiological abnormalities that result in increased cardiovascular risk. The primary aim of this proof‐of‐concept study was to analyse the existence of a potential link between Klotho gene polymorphisms and the expression level of this gene in the vascular wall, and additionally with the incidence of cardiovascular disease and cardiovascular risk factors. Our results indicate that the variant G‐395A, located in the promoter region, influences Klotho gene vascular expression and is associated with the incidence of diabetes. Similarly, the exonic variant KL‐VS was associated with the incidence of atherosclerotic vascular disease and coronary artery disease. Moreover, vascular expression levels of Klotho were related with the incidence of diabetes mellitus and coronary artery disease. These findings, which need to be confirmed in larger studies, suggest a potential role of Klotho in the pathogenesis of vascular damage.


Revista Espanola De Cardiologia | 2009

Venous cannula obstruction due to vegetative endocarditis.

Ibrahim Nassar; Ramiro de la Llana; Rafael Martínez-Sanz; Juan Lacalzada

Rev Esp Cardiol. 2009;62(8):941-54 949 Suberviola Cañas B, Rodríguez Borregan JC, González Castro A, Miñambres E, Burón Mediavilla FJ. Pericarditis purulenta y empiema pleural por Streptococcus pneumoniae. An Med Interna (Madrid). 2007;24:35-7. 4. Lewinter Martin M, Kabbani Samer. Pericardial diseases. In: Branwald E, editor. Heart Disease. A Textbook of Cardiovascular Medicine. 7.a ed. Eselvier Imprint; 2006. p. 1774.


The Annals of Thoracic Surgery | 2007

Multiple Overlapped Conical Endoprostheses in a Patient With Aneurysmatic Right Aortic Arch and Aortic Coarctation

M. Ibrahim Nassar; Ramiro de la Llana; Francisco Diaz-Romero; Pilar Garrido; Rafael Martínez-Sanz


The Journal of Thoracic and Cardiovascular Surgery | 2005

Combined surgical and endoprothesical approach to solve a thoracic aortic pseudoaneurysm 15 years after coarctation correction

Ramiro de la Llana; Rafael Martínez-Sanz; Francisco Diaz-Romero; Ibrahim Nassar; Pilar Garrido


Frontiers in Pathology and Genetics | 2013

Vascular Tumor Necrosis Factor-alpha Gene Expression in Human Aortic Valve Calcification---Expression of TNF-α in Calcified Aortic Valves

Javier Donate-Correa; Rafael Martínez-Sanz; Mercedes Muros-de-Fuentes; Carmen Mora-Fernández; Ernesto Martín-Núñez; Violeta Cazaña-Pérez; Ramiro de-la-Llana; Juan F. Navarro-González


Interactive Cardiovascular and Thoracic Surgery | 2012

eReply. Re: The problem of anaemia correction in cardiac surgery patients

Pilar Garriido Martin; Rafael Martínez-Sanz; Ramiro De La Llan-Ducros; Ibrahim Nassar-Mansur


F1000Research | 2012

Expression of FGF23/KLOTHO system in human vascular tissue

Javier Donate-Correa; Rafael Martínez-Sanz; Mercedes Muro-de-Fuentes; Horacio Pérez; Violeta Cazana; Carmen Mora-Fernández; Juan F. Navarro-González

Collaboration


Dive into the Rafael Martínez-Sanz's collaboration.

Top Co-Authors

Avatar

Ramiro de la Llana

Hospital Universitario de Canarias

View shared research outputs
Top Co-Authors

Avatar

Ibrahim Nassar

Hospital Universitario de Canarias

View shared research outputs
Top Co-Authors

Avatar
Top Co-Authors

Avatar

Juan F. Navarro-González

Spanish National Research Council

View shared research outputs
Top Co-Authors

Avatar

Pilar Garrido

Hospital Universitario de Canarias

View shared research outputs
Top Co-Authors

Avatar
Top Co-Authors

Avatar

Francisco Diaz-Romero

Hospital Universitario de Canarias

View shared research outputs
Top Co-Authors

Avatar

Juan Lacalzada

Hospital Universitario de Canarias

View shared research outputs
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Researchain Logo
Decentralizing Knowledge