Network


Latest external collaboration on country level. Dive into details by clicking on the dots.

Hotspot


Dive into the research topics where Rafael Queiroz de Souza is active.

Publication


Featured researches published by Rafael Queiroz de Souza.


American Journal of Infection Control | 2010

Analysis of the microbial load in instruments used in orthopedic surgeries

Flávia Morais Gomes Pinto; Rafael Queiroz de Souza; Cely Barreto da Silva; Lycia Mara Jenné Mimica; Kazuko Uchikawa Graziano

BACKGROUND Because of advances in technology, the number of orthopedic surgeries, mainly hip and knee replacement surgeries, has increased, with a total of 150,000 prosthetic surgeries estimated per year in the United States and 400,000 worldwide. METHODS We used an exploratory cross-sectional study, with a quantitative approach to determine the microbial load in instruments used in orthopedic surgeries, quantifying and identifying the microbial growth genus and species, according to the surgical potential of contamination that characterizes the challenge faced by the Material and Sterilization Center at the Institute of Orthopedics and Traumatology of Hospital das Clinicas of the School of Medicine of the University of Sao Paulo, Brazil.The orthopedic surgical instruments were immersed, after their use, in sterilized distilled water, sonicated in an ultrasonic washer, and posteriorly agitated. Subsequently, the wash was filtrated through a 0.45-mum membrane and incubated in aerobic and anaerobic mediums and in medium for fungi and yeasts. RESULTS In clean surgeries, 47% of the instruments were contaminated; in contaminated surgeries, 70%; and, in infected surgeries, 80%. Regardless of the contamination potential of the surgeries, the highest quantitative incidence of microorganism recovery was located in the 1 to 100 colony-forming unit range, and 13 samples presented a microbial growth potential >300 colony-forming units. Regardless of the contamination potential of the surgeries, there was a convergence in the incidence of negative-coagulase Staphylococcus growth (28%, clean surgeries; 32%, contaminated surgeries; and 29%, infected surgeries) and Staphylococcus aureus (28%, contaminated surgeries; and 43%, infected surgeries). CONCLUSION Most of the microorganisms recovered from the analyzed instruments (78%) were vegetative bacteria that presented their death curve at around 80 degrees C, characterizing a low challenge considering the processes of cleaning and sterilization currently employed by the Material and Sterilization Center. Fewer microorganisms were recovered from instruments used in clean surgeries in comparison with those used in contaminated and infected surgeries.


Revista Latino-americana De Enfermagem | 2013

Effectiveness of disinfection with alcohol 70% (w/v) of contaminated surfaces not previously cleaned

Maurício Uchikawa Graziano; Kazuko Uchikawa Graziano; Flávia Morais Gomes Pinto; Camila Quartim de Moraes Bruna; Rafael Queiroz de Souza; Cesar Angelo Lascala

OBJETIVO: avaliar a eficacia desinfetante do alcool 70% (p/v) sob friccao, sem limpeza previa, nas superficies de trabalho, como procedimento de desinfeccao concorrente em Servicos de Saude. METODO: foi desenvolvido estudo experimental laboratorial, randomizado e unicegado. As amostras foram constituidas de superficies esmaltadas, intencionalmente contaminadas com microrganismos Serratia marcescens ATCC 14756 106 Unidades Formadoras de Colonias/mL, acrescidos de 10% de saliva humana, e submetidas ao procedimento de desinfeccao sem limpeza previa. Os resultados foram comparados a desinfeccao precedida da limpeza. RESULTADOS: houve reducao de seis logaritmos da populacao microbiana inicial, igualmente nos grupos com e sem limpeza previa (p=0,440) e uma carga microbiana residual ≤ 102 Unidades Formadoras de Colonias. CONCLUSAO: a pesquisa demonstrou a aceitabilidade da pratica avaliada, trazendo importante resposta para a area da saude, especialmente a enfermagem, que mais executa os procedimentos de limpeza/desinfeccao concorrentes dessas superficies de trabalho.OBJECTIVE To evaluate the disinfectant effectiveness of alcohol 70% (w/v) using friction, without previous cleaning, on work surfaces, as a concurrent disinfecting procedure in Health Services. METHOD An experimental, randomized and single-blinded laboratory study was undertaken. The samples were enamelled surfaces, intentionally contaminated with Serratia marcescens microorganisms ATCC 14756 106 CFU/mL with 10% of human saliva added, and were submitted to the procedure of disinfection WITHOUT previous cleaning. The results were compared to disinfection preceded by cleaning. RESULTS There was a reduction of six logarithms of the initial microbial population, equal in the groups WITH and WITHOUT previous cleaning (p=0.440) and a residual microbial load ≤ 102 CFU. CONCLUSION The research demonstrated the acceptability of the practice evaluated, bringing an important response to the area of health, in particular to Nursing, which most undertakes procedures of concurrent cleaning /disinfecting of these work surfaces.


Journal of Cataract and Refractive Surgery | 2013

Cytotoxicity of cannulas for ophthalmic surgery after cleaning and sterilization: evaluation of the use of enzymatic detergent to remove residual ophthalmic viscosurgical device material.

Natacha S.M. Tamashiro; Rafael Queiroz de Souza; Cláudia Regina Gonçalves; Tamiko Ichikawa Ikeda; Reginaldo Adalberto Luz; Áurea S. Cruz; Maria Clara Padoveze; Kazuko Uchikawa Graziano

Purpose To evaluate the cytotoxicity of reusable cannulas for ophthalmic surgery after the cannulas were filled with an ophthalmic viscosurgical device (OVD) and cleaned with an enzymatic detergent. Setting Microbiological Testing Laboratory, Department of Medical‐Surgical Nursing, University of São Paulo School of Nursing, and Cell Culture Section, Adolfo Lutz Institute, São Paulo, Brazil. Design Experimental study. Methods The sample consisted of 30 reusable 25‐gauge injection cannulas, 20.0 mm in length, whose lumens were filled with an OVD solution for 50 minutes. The following steps were used to process the cannulas: (1) presoaking, (2) washing the lumen using a high‐pressure water jet, (3) backwashing with enzymatic detergent in ultrasonic cleaner, (4) preliminary rinsing with tap water, (5) final rinsing with sterile distilled water, (6) drying with compressed filtered air, (7) wrapping in surgical‐grade paper, and (8) steam sterilization at 134°C for 4 minutes. The cannulas were then tested for cytotoxicity according to the United States Pharmacopeia 32. Results The cleaning protocol used in this study removed residues of OVD solution and enzymatic detergent as shown by the lack of cytotoxicity of all sample extracts. Conclusion This cleaning protocol has the potential to minimize the occurrence of toxic anterior segment syndrome associated with residues of OVD solutions and enzymatic detergents. Financial Disclosure No author has a financial or proprietary interest in any material or method mentioned.


Revista Da Escola De Enfermagem Da Usp | 2015

O impacto do último enxágue na citotoxicidade de produtos críticos passíveis de processamento

Rafael Queiroz de Souza; Cláudia Regina Gonçalves; Tamiko Ichikawa Ikeda; Áurea Silveira Cruz; Kazuko Uchikawa Graziano

Objective: To assess the cytotoxicity of products subsequent to a cleaning process based on a validated standard operating procedure (SOP), and a final rinse with different types of water: tap, deionized, distilled, treated by reverse osmosis and ultra-purified. Method: This was an experimental and laboratory study. The sample consisted of 130 hydrodissection cannulas, 26 per experimental group, characterized according to type of water used in the final rinse. The samples were submitted to internal and external contamination challenge with a solution containing 20% defibrinated sheep blood and 80% of sodium chloride 0.9%. Next, the lumens were filled with a ophthalmic viscosurgical device, remaining exposed for 50 minutes, and then were processed according to the validated SOP. Cytotoxicity was assessed using neutral red uptake assay. Results: No cytoxicity was detected in the sample extracts. Conclusion: The samples did not display signs of cytotoxicity, regardless of final rinse quality. The results obtained were reached by using only a validated cleaning operating procedure, based on the scientific literature, and on official recommendations and related regulation.Objective To assess the cytotoxicity of products subsequent to a cleaning process based on a validated standard operating procedure (SOP), and a final rinse with different types of water: tap, deionized, distilled, treated by reverse osmosis and ultra-purified. Method This was an experimental and laboratory study. The sample consisted of 130 hydrodissection cannulas, 26 per experimental group, characterized according to type of water used in the final rinse. The samples were submitted to internal and external contamination challenge with a solution containing 20% defibrinated sheep blood and 80% of sodium chloride 0.9%. Next, the lumens were filled with a ophthalmic viscosurgical device, remaining exposed for 50 minutes, and then were processed according to the validated SOP. Cytotoxicity was assessed using neutral red uptake assay. Results No cytoxicity was detected in the sample extracts. Conclusion The samples did not display signs of cytotoxicity, regardless of final rinse quality. The results obtained were reached by using only a validated cleaning operating procedure, based on the scientific literature, and on official recommendations and related regulation.


Revista Latino-americana De Enfermagem | 2013

Comparison of hand hygiene antimicrobial efficacy: Melaleuca alternifolia essential oil versus triclosan

Juliana Rizzo Gnatta; Flávia Morais Gomes Pinto; Camila Quartim de Moraes Bruna; Rafael Queiroz de Souza; Kazuko Uchikawa Graziano; Maria Júlia Paes da Silva

OBJETIVOS: este estudio objetivo evaluar la eficacia de la higienizacion de las manos realizada con dos formulas diferentes de jabones: aceite esencial de Melaleuca alternifolia al 0,3% versus triclosan al 0,5%, y compararlas con dos higienizaciones de referencia: procedimiento de la metodologia oficial (soft soap) versus procedimiento de la version draft (soft soap + propan-2-ol). METODO: utilizando la metodologia europea EN 1499, fueron determinados factores de reduccion logaritmica del numero de unidades formadoras de colonia de Escherichia coli K12 antes y despues de la higiene de las manos de 15 sujetos voluntarios y comparados por el test de Wilcoxon de una sola cola. RESULTADOS: Tomando como referencia el soft soap, no hubo diferencia entre el desempeno del jabon con Melaleuca alternifolia al 0,3% o jabon conteniendo triclosan al 0,5%. El soft soap + propan-2-ol se demostro mas eficaz en relacion a los demas procedimientos de higienizacion de las manos. CONCLUSION: estudios que verifiquen la eficacia terapeutica de aceite esencial en la higienizacion de las manos pueden mejorar la adhesion a esa practica.OBJECTIVE this study aimed to evaluate the efficacy of hand hygiene performed with two different soap formulations: 0.3% Melaleuca alternifolia essential oil versus 0.5% triclosan, and to compare them with two reference hygiene procedures: the official methodology procedure (soft soap) versus the draft version of the procedure (soft soap + propan-2-ol). METHOD using the European EN 1499 method, logarithmic reduction factors were determined for the number of colony forming units of Escherichia coli K12 before and after hand hygiene of 15 volunteer subjects, and compared using the one-tailed Wilcoxon test. RESULTS referring to the soft soap, there was no difference between the performance of soap with 0.3% M. alternifolia and soap containing 0.5% triclosan. The soft soap + propan-2-ol proved to be more effective than the other hand hygiene procedures. CONCLUSION studies to verify the therapeutic efficacy of essential oil in hand hygiene can improve adherence to this practice.


Revista Da Escola De Enfermagem Da Usp | 2012

Micro-organismos da subclasse Coccidia: resistência e implicações para o processamento de materiais de assistência à saúde

Rafael Queiroz de Souza; Lilian Machado Torres; Kazuko Uchikawa Graziano; Ruth Natalia Teresa Turrini

This theoretical study proposes a reflection on the intrinsic resistance of the subclass Coccidia, particularly the genus Cryptosporidium, considered to be potential pathogens for immunocompromised patients, and the implications for nursing practice. Currently, the international and national guidelines support the chemical disinfection of digestive system endoscopes after their cleansing as a safe and effective procedure. However, studies show that microorganisms of the subclass Coccidia, namely Cryptosporidium, responsible for enteric infection, are more resistant than mycobacteria and are not inactivated by high-level disinfectants, except for hydrogen peroxide 6% and 7.5%, which are not currently available in Brazil. We conclude that the legislation should include this agent among test microorganisms for approving high-level disinfectants. Health authorities should make efforts to ensure that healthcare institutions have access to effective disinfectants against Cryptosporidium.This theoretical study proposes a reflection on the intrinsic resistance of the subclass Coccidia, particularly the genus Cryptosporidium, considered to be potential pathogens for immunocompromised patients, and the implications for nursing practice. Currently, the international and national guidelines support the chemical disinfection of digestive system endoscopes after their cleansing as a safe and effective procedure. However, studies show that microorganisms of the subclass Coccidia, namely Cryptosporidium, responsible for enteric infection, are more resistant than mycobacteria and are not inactivated by high-level disinfectants, except for hydrogen peroxide 6% and 7.5%, which are not currently available in Brazil. We conclude that the legislation should include this agent among test microorganisms for approving high-level disinfectants. Health authorities should make efforts to ensure that healthcare institutions have access to effective disinfectants against Cryptosporidium.


Revista Da Escola De Enfermagem Da Usp | 2012

Evaluation of microbial growth on single-use vitrectomy probes reprocessed in healthcare practice

Flávia Morais Gomes Pinto; Valéria Garcia Lopes Araújo; Rafael Queiroz de Souza; Vânia Regina Goveia; Carmen Castilho Missali; Reginaldo Adalberto Luz; Kazuko Uchikawa Graziano

O objetivo deste estudo foi avaliar o crescimento microbiano em sondas para vitrectomia de uso unico, reprocessadas na pratica assistencial. Foram investigadas nove sondas reusadas e reprocessadas por diferentes metodos. As sondas foram segmentadas, individualmente, em porcoes de 3,5 cm, totalizando em 979 unidades amostrais (extensoes, conectores e ponteiras) inoculadas em meio de cultura e incubadas a 37oC, por 14 dias. Os resultados mostraram crescimento microbiano em 57 (5,8%) unidades amostrais, das quais, 25 foram esterilizadas por Oxido de Etileno, 16 por Plasma de Peroxido de Hidrogenio e 16 por Vapor a Baixa Temperatura e Formaldeido. Foram identificadas 17 especies microbianas, sendo as mais prevalentes o Micrococcus spp., Staphylococcus coagulase negativa, Pseudomonas spp. e Bacillus subtilis. O reuso de sondas de uso unico para vitrectomia nao se mostrou seguro, portanto tal pratica nao e recomendada.The aim of this study was to evaluate the microbial growth on single-use vitrectomy probes reprocessed in healthcare practice. We investigated nine vitrectomy probes that had been reused and reprocessed using different methods. The samples were sectioned, individually, in portions of 3.5 cm, totaling 979 sampling units (extensions, connectors and vitrectomy cutters), which were inoculated in culture medium and incubated at 37º C for 14 days. The results showed microbial growth on 57 (5.8%) sample units, 25 of which had been sterilized using ethylene oxide, 16 by hydrogen peroxide plasma, and 16 by low-temperature steam and formaldehyde. Seventeen microbial species were identified. The most prevalent were: Micrococcus spp., coagulase-negative Staphylococcus, Pseudomonas spp., and Bacillus subtilis. The reuse of single-use vitrectomy probes was shown to be unsafe, therefore this practice is not recommended.


Revista Latino-americana De Enfermagem | 2013

Eficácia da desinfecção com álcool 70% (p/v) de superfícies contaminadas sem limpeza prévia

Maurício Uchikawa Graziano; Kazuko Uchikawa Graziano; Flávia Morais Gomes Pinto; Camila Quartim de Moraes Bruna; Rafael Queiroz de Souza; Cesar Angelo Lascala

OBJETIVO: avaliar a eficacia desinfetante do alcool 70% (p/v) sob friccao, sem limpeza previa, nas superficies de trabalho, como procedimento de desinfeccao concorrente em Servicos de Saude. METODO: foi desenvolvido estudo experimental laboratorial, randomizado e unicegado. As amostras foram constituidas de superficies esmaltadas, intencionalmente contaminadas com microrganismos Serratia marcescens ATCC 14756 106 Unidades Formadoras de Colonias/mL, acrescidos de 10% de saliva humana, e submetidas ao procedimento de desinfeccao sem limpeza previa. Os resultados foram comparados a desinfeccao precedida da limpeza. RESULTADOS: houve reducao de seis logaritmos da populacao microbiana inicial, igualmente nos grupos com e sem limpeza previa (p=0,440) e uma carga microbiana residual ≤ 102 Unidades Formadoras de Colonias. CONCLUSAO: a pesquisa demonstrou a aceitabilidade da pratica avaliada, trazendo importante resposta para a area da saude, especialmente a enfermagem, que mais executa os procedimentos de limpeza/desinfeccao concorrentes dessas superficies de trabalho.OBJECTIVE To evaluate the disinfectant effectiveness of alcohol 70% (w/v) using friction, without previous cleaning, on work surfaces, as a concurrent disinfecting procedure in Health Services. METHOD An experimental, randomized and single-blinded laboratory study was undertaken. The samples were enamelled surfaces, intentionally contaminated with Serratia marcescens microorganisms ATCC 14756 106 CFU/mL with 10% of human saliva added, and were submitted to the procedure of disinfection WITHOUT previous cleaning. The results were compared to disinfection preceded by cleaning. RESULTS There was a reduction of six logarithms of the initial microbial population, equal in the groups WITH and WITHOUT previous cleaning (p=0.440) and a residual microbial load ≤ 102 CFU. CONCLUSION The research demonstrated the acceptability of the practice evaluated, bringing an important response to the area of health, in particular to Nursing, which most undertakes procedures of concurrent cleaning /disinfecting of these work surfaces.


Revista Da Escola De Enfermagem Da Usp | 2010

Compatibilidades e incompatibilidades entre radiação gama e óxido de etileno como métodos sucessivos de esterilização

Rafael Queiroz de Souza; Kazuko Uchikawa Graziano

A duvida sobre a questao da re-esterilizacao de artigos confeccionados com cloreto de polivinila (PVC), esterilizados em radiacao gama (RG) e posteriormente em oxido de etileno (EO) persiste ate os dias atuais. Por meio da revisao integrativa da literatura, este artigo analisou estudos que evidenciaram compatibilidades e incompatibilidades entre os processos de esterilizacao com RG e EO quando utilizados como metodos sucessivos de esterilizacao. Foram analisados 7 estudos. Constatou-se que ha influencia multifatorial na seguranca do procedimento e que o metodo analitico da cromatografia empregado na maioria dos estudos produziu resultados controversos. Esse fato indica a necessidade da realizacao de novos estudos sobre o assunto, utilizando-se metodos analiticos mais sensiveis do que a cromatografia gasosa, como o teste de reatividade biologica em culturas celulares, que podera dirimir em nosso meio a cronica duvida da compatibilidade ou incompatibilidade de se esterilizar em EO materiais previamente gamairradiados.


Revista SOBECC | 2017

Avaliação da segurança do processamento de fresas intramedulares flexíveis Para cirurgia ortopédica

Rafael Queiroz de Souza; Jeane Aparecida Gonzalez Bronzatti; Paulo Roberto Laranjeira; Lycia Mara Jenné Mimica; Cely Barreto da Silva; Áurea Silveira Cruz; Kazuko Uchikawa Graziano

Objectives: To assess the efficacy of a standard operational procedure to clean flexible intramedullary bone reamers, as well as the sterilization level, and to show the cytotoxicity of the residual dirtiness of a flexible reamer used in care practice. Methods: Flexible intramedullary bone reamers were weighed before processing, after challenge contamination and after cleaning. They were contaminated with the Soil Test™, Geobacillus stearothermophilus suspension, in the concentration of 106 cfu/ml, and bovine bone flour. After processing, the samples were inoculated into a culture medium and incubated for 21 days. Residual dirtiness of a flexible intramedullary bone reamer used in practice was submitted to in vitro cytotoxicity test. Results: Despite being sterilized, the samples indicate to accumulated dirtiness and the processing was inefficient. Residual dirtiness presented a cytotoxic effect. Conclusion: It is recommended that the flexible design of reamers is discontinued by the lack of safety of reprocessing.

Collaboration


Dive into the Rafael Queiroz de Souza's collaboration.

Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Researchain Logo
Decentralizing Knowledge