Raffaele Leone

Study of quantitative and qualitative variations in essential oils of Sicilian Rosmarinus officinalis L.

In this study the chemical characterisation of 10 Sicilian Rosmarinus officinalis L. biotypes essential oils is reported. The main goal of this work was to analyse the relationship between the essential oils yield and the geographical distribution of the species plants. The essential oils were analysed by GC-FID and GC-MS. Hierarchical cluster analysis and principal component analysis statistical methods were used to cluster biotypes according to the essential oils chemical composition. The essential oil yield ranged from 0.8 to 2.3 (v/w). In total 82 compounds have been identified, these represent 96.7–99.9% of the essential oil. The most represented compounds in the essential oils were 1.8-cineole, linalool, α-terpineol, verbenone, α-pinene, limonene, bornyl acetate and terpinolene. The results show that the essential oil yield of the 10 biotypes is affected by the environmental characteristics of the sampling sites while the chemical composition is linked to the genetic characteristics of different biotypes.

Study of quantitative and qualitative variations in essential oils of Sicilian oregano biotypes

Essential oil (EO) was extracted using hydrodistillation from samples of Origanum vulgare subspecies hirtum (Link) Ietswaart, gathered from the wild in various parts of Sicily, Italy; GC-FID and GC-MS analyses were subsequently performed. The aim of the study was to analyze the relationship between essential oil yields and the geographical distribution of oregano wild populations based on variations in environmental factors as collection sites. Moreover, the purpose was to group Origanum vulgare subspecies hirtum biotypes according to the chemical composition of the EO. The seven principal components in the EO was thymol (24.0–54.4%), γ-terpinene (9.8–30.5%), ρ-cymene (5.2–18.7%), α-terpinene (2.7–5.7%), carvacrol (0.3–8.3%), terpinen-4-ol (0.5–9.4%) and trans-sabinene hydrate (0.0–12.8%). All the biotypes analyzed were identified as thymol-chemotype. Statistical analysis shows that essential oil yields are mostly affected by weather/climate and topographic characteristics of the collection sites.

Agronomical and chemical characterisation of Thymbra capitata (L.) Cav. biotypes from Sicily, Italy

In this study, the agronomical and chemical characterisation of 13 Sicilian biotypes of Thymbra capitata (L.) Cav., grown under the same agricultural and environmental condition, are reported. The main morpho-productive parameters and quali-quantitative profile of essential oils (EOs) were determined. The EOs were analysed by gas chromatography-flame ionisation detector and gas chromatography–mass spectrometry. Hierarchical cluster analysis and principal component analysis statistical methods were used to group biotypes according to the EOs chemical composition. The EO yield ranged between 4.6 and 8.1 (v/w). A total of 38 EO compounds have been identified. The compounds mostly represented were α-pinene, myrcene, α-terpinene, p-cymene, γ-terpinene, borneol, carvacrol and β-caryophyllene. In all biotypes, the carvacrol (67.4–79.5%) was the main compound, confirming that T. capitata is a carvacrol chemotype. The results showed that all Sicilian Thymbra biotypes have a good adaptation to the climatic conditions of the test environment.

Essential oil characteristics of wild Sicilian oregano populations in relation to environmental conditions

A study was carried out on the essential oil of Origanum vulgare ssp. hirtum (Link) Ietswaart taken from thirteen wild populations found in different parts of Sicily, Italy. The aim of the study was to evaluate the relationship that exists between essentials oil extracted from Sicilian wild populations of O. vulgare ssp. hirtum (Link) Ietswaart and various weather/climate and topographic factors in order to provide more information on aspects that influence the production potential of this species. The essential oils from the samples were extracted by hydrodistillation and analyzed by a combination of gas chromatography–flame ionization detector (GC–FID) and GC–mass spectrometry (GC–MS); seventy-one components were fully identified, however, three main components were characteristic of the examined samples accounting for a significant amount of all oils: thymol (24.0–54.4%), γ-terpinene (9.8–30.5%) and p-cymene (5.2–18.7%). Analyses highlighted a different qualitative and quantitative essential oil composition compared with other wild populations in the Mediterranean area and in relation to the different weather/climate and topographic characteristics of the collection sites. These differences are probably due to a mixture of genetic and environmental factors, which influence secondary metabolite biosynthesis: the basis of essential oils.

Wild Sicilian Rosemary: Phytochemical and Morphological Screening and Antioxidant Activity Evaluation of Extracts and Essential Oils

To identify the best biotypes, an extensive survey of Sicilian wild rosemary was carried out by collecting 57 samples from various sites, followed by taxonomic characterization from an agronomic perspective. All the biotypes collected were classified as Rosmarinus officinalis L. A cluster analysis based on the morphological characteristics of the plants allowed the division of the biotypes into seven main groups, although the characteristics examined were found to be highly similar and not area‐dependent. Moreover, all samples were analyzed for their phytochemical content, applying an extraction protocol to obtain the nonvolatile components and hydrodistillation to collect the essential oils for the volatile components. The extracts were characterized by LC‐UV‐DAD/ESI‐MS, and the essential oils by GC‐FID and GC/MS analyses. In the nonvolatile fractions, 18 components were identified, namely, 13 flavones, two organic acids, and three diterpenes. In the volatile fractions, a total of 82 components were found, with as predominant components α‐pinene and camphene among the monoterpene hydrocarbons and 1,8‐cineole, camphor, borneol, and verbenone among the oxygenated monoterpenes. Cluster analyses were carried out on both phytochemical profiles, allowing the separation of the rosemary samples into different chemical groups. Finally, the total phenol content and the antioxidant activity of the essential oils and extracts were determined with the Folin–Ciocalteu (FC) colorimetric assay, the UV radiation‐induced peroxidation in liposomal membranes (UV‐IP test), and the scavenging activity of the superoxide radical (O

Growth of Arundo donax L. and Cyperus alternifolius L. in a horizontal subsurface flow constructed wetland using pre-treated urban wastewater—a case study in Sicily (Italy)

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Agronomical evaluation of Sicilian biotypes of Lavandula stoechas L. spp. stoechas and analysis of the essential oils

). The present study confirmed that the essential oils and organic extracts of the Sicilian rosemary samples analyzed showed a considerable antioxidant/free radical‐scavenging activity.

Effects of plant species in a horizontal subsurface flow constructed wetland – phytoremediation of treated urban wastewater with Cyperus alternifolius L. and Typha latifolia L. in the West of Sicily (Italy)

AbstractConstructed wetland systems for wastewater treatment represent an excellent opportunity for the recovery of water resources in those areas subject to prolonged periods of water shortage. This paper presents a study of the efficiency of a pilot horizontal subsurface flow system using pre-treated urban wastewater from a small town in the West of Sicily. The pilot system had a total surface area of 100 m2 with two units. Unit A was planted with Arundo donax L. and unit B with Cyperus alternifolius L. The HLR was 10 cm d−1. The main objectives of research were to evaluate the growth and biomass yield of two macrophytes and to determine the removal efficiency of physical–chemical and microbiological parameters. The results showed excellent organic pollutant removal (BOD5 70–72%, COD 61–67%), whilst macronutrient removal was found to be lower (TKN 47–50%, TP 43–45%). Pathogen load removal was found to be approximately 90%, but Escherichia coli concentrations at outflow were not within Italian legislativ...

Effect of plant species on water balance in a pilot-scale horizontal subsurface flow constructed wetland planted with Arundo donax L. and Cyperus alternifolius L. – Two-year tests in a Mediterranean environment in the West of Sicily (Italy)

The aim of this study was to characterize wild lavender, which was collected in three different areas of Sicily (Italy), according to agronomic and chemical evaluation. The collection sites were located in Pantelleria island, Partinico (a warm sub-area of Lauretum) and Castelbuono (a middle sub-area of Lauretum). All the populations were identified as Lavandula stoechas L. ssp. stoechas. Essential oils were extracted by hydrodistillation and analyzed by gas chromatography–flame ionization detector (GC–FID) and GC–mass spectrometry (GC–MS). GC–FID and GC–MS analyses permitted the identification of 101 components from the essential oils. We analyzed only the flowers and leaves of L. stoechas and the samples were analyzed using the PCA (principal component analysis) methodology regarding the chemical composition of the essential oils. Comparisons were carried out between the chemical compositions of essential oils from Sicilian populations and other Mediterranean populations. The essential oils of Sicilian L. stoechas biotypes were fenchone chemotype with percentages ranging from 45.29% to 60.27%. The qualitative chemical composition of the essential oils varied according to the different areas of origin of the plant material. Sicilian biotypes of L. stoechas showed high differences in chemical composition compared with the populations coming from other Mediterranean areas.