Ragia M. Hegazy

Cardiorenal Effects of Newer NSAIDs (Celecoxib) versus Classic NSAIDs (Ibuprofen) in Patients with Arthritis

Background. Arthritis is a common condition that co-exists in the elderly population. This condition leads to frequent administration of comorbid analgesics especially non steroidal anti-inflammatory drugs (NSAIDs). Aim. To study cardiorenal toxicity of celecoxib versus ibuprofen in arthritic patients. Subjects and Methods. Seven hundred ninety-wo arthritic patients were enrolled in the study for 6 months. Three hundred ninety-six patients administered celecoxib 400 mg twice a day; 396 patients administered ibuprofen 300 mg three times a day. Effects measured included investigator-reported hypertension, edema, or congestive heart failure, increases in serum creatinine or reduction in serum creatinine clearance, and changes in serum electrolytes. Results. Celecoxib was associated with significant (P < .05) lower incidence of hypertension and edema in comparison with ibuprofen. Systolic hypertension occurred significantly less (P < .05) with celecoxib compared with ibuprofen. Serum creatinine was significantly increased (P < .05) in patients treated with ibuprofen in comparison with celecoxib. Creatinine clearance was significantly lower (P < .05) in cases treated with ibuprofen in comparison to celecoxib. Nonsignificant changes in serum body electrolytes occurred. Conclusion. The most important finding of this study was the lowering incidence of cardiorenal toxicity of celecoxib in comparison with ibuprofen.

Estimation of Cytotoxicity and Genotoxicity of long acting Bronchodilator: Salmeterol Xinafoate Nanoparticles (Nanotoxicity Study)

Nanoparticles (NPs) are now extensively used in different fields with inadequate knowledge on their toxicity. Salmeterol is a potent long acting-β agonist (LABA) used for the management of pulmonary disorders. Sothe present study was aimed to evaluating in vitro cytotoxicity and genotoxicity of nano salmeterol xinafoate nebulization. Blood samples from ten healthy volunteers were cultured and divided into two equal groups: control untreated group and other treated with a dose of nano salmeterol (50μg/ml) and incubated at 37°C for 24 hour then evaluated for local and systemic toxicity. Genotoxic study by using Comet assay and chromosomal karyotype was done. Morphological analysis of nano-sized salmeterol xinafoate particles was performed by Transmission Electron Microscopy (TEM) and Anderson Cascade Impactor (ACI) in which the average particle size was in the range of 300–500 nm. Our results showed that there was anon significant decrease (p>0.0.05) in the level of antioxidant enzymes: ROS, GSH, SOD, and Catalase; while, there was significant increase (p<0.0.05) in the level of Lipid Peroxidation (LPO). The Comet assay indicated that cells treated with Salmeterol xinafoate NP induced non-significant increase in percentage tail DNA damage compared to control (p>0.0.05), Also, the chromosomal aberration was non-significant increase (p>0.0.05). So our study concluded that this dose of nano-salmeterol xinafoate NP nebulizer may be tolerated by asthmatic patients and further studies need in diseased patients and with different organs.

Histopathological and immunohistochemical study of the toxicity of UVB-irradiation on rabbit's cornea: possible antioxidant role of Trehalose.

Aims: ThisStudyaimed toevaluate toxic changes that might occur in rabbit cornea after UVB exposure and possible protective role of Trehalose. Study Design: Eighteen adult white female rabbits were divided into three groups, six rabbits for each one. Group �o received buffered saline (negative control), Group �o�o irradiated by UVB (positive control) and Group �o�oI irradiated by UVB with concomitant application of Trehalose eye drops.