Raja Hussain Ali
Quaid-i-Azam University
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Featured researches published by Raja Hussain Ali.
BMC Medical Genetics | 2012
Saadullah Khan; Raja Hussain Ali; Sanaullah Abbasi; Muhammad Nawaz; Noor Muhammad; Wasim Ahmad
BackgroundNatriuretic peptides (NPs) are peptide hormones that exert their biological actions by binding to three types of cell surface natriuretic peptide receptors (NPRs). The receptor NPR-B binding C-type natriuretic peptide (CNP) acts locally as a paracrine and/or autocrine regulator in a wide variety of tissues. Mutations in the gene NPR2 have been shown to cause acromesomelic dysplasia-type Maroteaux (AMDM), an autosomal recessive skeletal disproportionate dwarfism disorder in humans.MethodsIn the study, presented here, genotyping of six consanguineous families of Pakistani origin with AMDM was carried out using polymorphic microsatellite markers, which are closely linked to the gene NPR2 on chromosome 9p21-p12. To screen for mutations in the gene NPR2, all of its coding exons and splice junction sites were PCR amplified from genomic DNA of affected and unaffected individuals of the families and sequenced.ResultsSequence analysis of the gene NPR2 identified a novel missence mutation (p.T907M) in five families, and a splice donor site mutation c.2986 + 2 T > G in the other family.ConclusionWe have described two novel mutations in the gene NPR2. The presence of the same mutation (p.T907M) and haplotype in five families (A, B, C, D, E) is suggestive of a founder effect.
Human Molecular Genetics | 2014
Regie Lyn P. Santos-Cortez; Kwanghyuk Lee; Arnaud P. Giese; Muhammad Ansar; Muhammad Amin-ud-din; Kira Rehn; Xin Wang; Abdul Aziz; Ilene Chiu; Raja Hussain Ali; Joshua D. Smith; Jay Shendure; Michael J. Bamshad; Deborah A. Nickerson; Zubair M. Ahmed; Wasim Ahmad; Saima Riazuddin; Suzanne M. Leal
Cyclic AMP (cAMP) production, which is important for mechanotransduction within the inner ear, is catalyzed by adenylate cyclases (AC). However, knowledge of the role of ACs in hearing is limited. Previously, a novel autosomal recessive non-syndromic hearing impairment locus DFNB44 was mapped to chromosome 7p14.1-q11.22 in a consanguineous family from Pakistan. Through whole-exome sequencing of DNA samples from hearing-impaired family members, a nonsense mutation c.3112C>T (p.Arg1038*) within adenylate cyclase 1 (ADCY1) was identified. This stop-gained mutation segregated with hearing impairment within the family and was not identified in ethnically matched controls or within variant databases. This mutation is predicted to cause the loss of 82 amino acids from the carboxyl tail, including highly conserved residues within the catalytic domain, plus a calmodulin-stimulation defect, both of which are expected to decrease enzymatic efficiency. Individuals who are homozygous for this mutation had symmetric, mild-to-moderate mixed hearing impairment. Zebrafish adcy1b morphants had no FM1-43 dye uptake and lacked startle response, indicating hair cell dysfunction and gross hearing impairment. In the mouse, Adcy1 expression was observed throughout inner ear development and maturation. ADCY1 was localized to the cytoplasm of supporting cells and hair cells of the cochlea and vestibule and also to cochlear hair cell nuclei and stereocilia. Ex vivo studies in COS-7 cells suggest that the carboxyl tail of ADCY1 is essential for localization to actin-based microvilli. These results demonstrate that ADCY1 has an evolutionarily conserved role in hearing and that cAMP signaling is important to hair cell function within the inner ear.
Clinical and Experimental Dermatology | 2015
Asmat Ullah; Syed Irfan Raza; Raja Hussain Ali; A. K. Naveed; Abid Jan; S. D. A. Rizvi; R. Satti; Wasim Ahmad
Autosomal recessive hypotrichosis is a rare human hereditary disorder presenting as sparse scalp hair or as woolly hair occurring on various parts of the body. Various forms of isolated hypotrichosis have been reported to date. Mutations in at least 11 genes have been reported to cause hypotrichosis.
PLOS ONE | 2014
Syed Irfan Raza; Dost Muhammad; Abid Jan; Raja Hussain Ali; Mubashir Hassan; Wasim Ahmad; Sajid Rashid
Autosomal recessive hypotrichosis is a rare genetic irreversible hair loss disorder characterized by sparse scalp hair, sparse to absent eyebrows and eyelashes, and sparse axillary and body hair. The study, presented here, established genetic linkage in four families showing similar phenotypes to lysophosphatidic acid receptor 6 (LPAR6) gene on chromosome 13q14.11-q21.32. Subsequently, sequence analysis of the gene revealed two previously reported missense mutations including p.D63V in affected members of one and p.I188F in three other families. Molecular modeling and docking analysis was performed to investigate binding of a ligand oleoyl-L-alpha-lysophosphatidic acid (LPA) to modeled protein structures of normal and mutated (D63V, G146R, I188F, N248Y, S3T, L277P) LPAR6 receptors. The mutant receptors showed a complete shift in orientation of LPA at the binding site. In addition, hydropathy analysis revealed a significant change in the membrane spanning topology of LPAR6 helical segments. The present study further substantiated involvement of LPAR6-LPA signaling in the pathogenesis of hypotrichosis/woolly hair and provided additional insight into the molecular mechanism of hair development.
European Journal of Dermatology | 2016
Farooq Ahmad; Raja Hussain Ali; Dost Muhammad; Abdul Nasir; Muhammad Umair; Salma M. Wakil; Khushnooda Ramzan; Sulman Basit; Wasim Ahmad
Human juvenile macular dystrophy (HJMD) results from a rare autosomal recessive genetic anomaly, manifesting with hypotrichosis and gradual loss of vision due to progressive macular degeneration. Variants in the CDH3 gene, encoding a trans-membrane glycoprotein P-cadherin, have been reported to result in HJMD [1] and its closely associated form, ectodermal dysplasia, ectrodactyly, macular dystrophy (EEM) [2].In the present study, two consanguineous families, A and B (figures 1A, B), in which HJMD [...]
Congenital Anomalies | 2017
Muhammad Umair; Afzal Rafique; Asmat Ullah; Farooq Ahmad; Raja Hussain Ali; Abdul Nasir; Muhammad Ansar; Wasim Ahmad
Acromesomelic dysplasia Grebe type (AMDG) is characterized by severe knob like non‐functional fingers and short acromesomelic limbs, and is inherited in an autosomal recessive manner. Disease causing sequence variants in the GDF5 (Growth Differentiation Factor 5) gene located on chromosome 20q11.22 are responsible for causing AMDG. In the study, presented here, two consanguineous families with AMDG were clinically and genetically characterized. After establishing linkage in the two families (A and B) to GDF5 gene on chromosome 20q11.22, Sanger DNA sequencing was performed in all available affected and unaffected members. Sequence analysis of the GDF5 gene revealed two novel variants including a duplication (c.157_158dupC, p.Leu53Profs*41) in family A, and a nonsense (p.Trp291*) in family B. Our findings extend the body of evidence that supports the importance of GDF5 in the development of limbs.
Clinical Genetics | 2016
Raja Hussain Ali; Khadim Shah; Abdul Nasir; Wouter Steyaert; Paul Coucke; Wasim Ahmad
Woodhouse Sakati syndrome (WSS, MIM 241080) is a rare autosomal recessive genetic condition characterized by alopecia, hypogonadism, hearing impairment, diabetes mellitus, learning disabilities and extrapydamidal manifestations. Sequence variants in the gene DCAF17, encoding nucleolar substrate receptor, were identified as the underlying cause of inherited WSS. Considerable phenotypic heterogeneity exists in WSS with regard to severity, organs involvement and age of onset, both in inter‐familial and intra‐familial cases. In this study, the genetic characterization of a consanguineous pedigree showing mild features of WSS was performed, followed by structural analysis of truncated protein. Exome sequencing identified a novel single base deletion variant (c.270delA; K90Nfs8*) in third exon of the gene DCAF17 (RefSeq; NM_025000), resulting in a truncated protein. Structural analysis of truncated DCAF17 revealed absence of amino acid residues crucial for interaction with DDB1. Taken together, the data confirmed the single base pair deletion as the underlying cause of this second report of WSS from Pakistan. This signifies the vital yet unexplored role of DCAF17 both in development and maintenance of adult tissues homeostasis.
International Journal of Dermatology | 2017
Khadim Shah; Sabba Mehmood; Abid Jan; Izoduwa Abbe; Raja Hussain Ali; Anwar Khan; Muhammad Salman Chishti; Kwanghyuk Lee; Farooq Ahmad; Muhammad Ansar; Shaheen Shahzad; Deborah A. Nickerson; Michael J. Bamshad; Paul Coucke; Regie Lyn P. Santos-Cortez; Richard A. Spritz; Suzanne M. Leal; Wasim Ahmad
Genodermatoses represent genetic anomalies of skin tissues including hair follicles, sebaceous glands, eccrine glands, nails, and teeth. Ten consanguineous families segregating various genodermatosis phenotypes were investigated in the present study.
PLOS ONE | 2016
Dara K. Mohammad; Raja Hussain Ali; Janne J. Turunen; Beston F. Nore; C. I. Edvard Smith
Protein kinase B (AKT) phosphorylates numerous substrates on the consensus motif RXRXXpS/T, a docking site for 14-3-3 interactions. To identify novel AKT-induced phosphorylation events following B cell receptor (BCR) activation, we performed proteomics, biochemical and bioinformatics analyses. Phosphorylated consensus motif-specific antibody enrichment, followed by tandem mass spectrometry, identified 446 proteins, containing 186 novel phosphorylation events. Moreover, we found 85 proteins with up regulated phosphorylation, while in 277 it was down regulated following stimulation. Up regulation was mainly in proteins involved in ribosomal and translational regulation, DNA binding and transcription regulation. Conversely, down regulation was preferentially in RNA binding, mRNA splicing and mRNP export proteins. Immunoblotting of two identified RNA regulatory proteins, RBM25 and MEF-2D, confirmed the proteomics data. Consistent with these findings, the AKT-inhibitor (MK-2206) dramatically reduced, while the mTORC-inhibitor PP242 totally blocked phosphorylation on the RXRXXpS/T motif. This demonstrates that this motif, previously suggested as an AKT target sequence, also is a substrate for mTORC1/2. Proteins with PDZ, PH and/or SH3 domains contained the consensus motif, whereas in those with an HMG-box, H15 domains and/or NF-X1-zinc-fingers, the motif was absent. Proteins carrying the consensus motif were found in all eukaryotic clades indicating that they regulate a phylogenetically conserved set of proteins.
Journal of Dermatological Science | 2015
Raja Hussain Ali; Sabba Mahmood; Syed Irfan Raza; Abdul Aziz; Irfanullah; Syed Kamran-ul-Hassan Naqvi; Naveed Wasif; Muhammad Ansar; Wasim Ahmad; Sayed Hajan Shah; Bakht Tarin Khan; Qaiser Zaman; Ajab Gul; Abdul Wali; Ghazanfar Ali; Saadulah Khan; Muhammad Khisroon; Sulman Basit
• We presented the first report of Xp22.3 micro-deletions underlying isolated XLI in seventeen familial cases representing major ethnic groups living in Pakistan.