Rajesh Asthana

Vertical distribution of bacteria in a lake sediment from Antarctica by culture-independent and culture-dependent approaches

Bacterial diversity of the subsurface (18-22 cm), middle (60-64 cm) and bottom (100-104 cm) of a 136-cm-long sediment core sampled from a freshwater lake in Antarctica was determined by the culturable approach, T-RFLP and 16S rRNA gene clone libraries. Using the culturable approach, 41 strains were isolated and, based on phylogenetic analysis, they could be categorized into 14 groups. Representatives of the 14 groups varied in their growth temperature range (4-30 °C), in their tolerance to NaCl (0-2 M NaCl) and in the growth pH range (5-11). Eleven of fourteen representative strains exhibited either amylase, lipase, protease and (or) urease activities at 4 °C. Bacterial diversity at the phyla level using T-RFLP and 16S rRNA clone libraries was similar and clones were affiliated with Proteobacteria, Bacteroidetes, Actinobacteria and Firmicutes. TRFs affiliated with Spirochaetes were detected only by the T-RFLP approach and clones affiliated with Caldiserica only in the clone libraries. Stratification of bacteria along the depth of the sediment was observed both with the T-RFLP and the 16S rRNA gene clone library methods, and results indicated that stratification was dependent on the nature of the organism, aerobic or anaerobic. For instance, aerobic Janthinobacterium and Polaromonas were confined to the surface of the sediment, whereas anaerobic Caldisericum was present only in the bottom portion of the core. It may be concluded that the bacterial diversity of an Antarctic lake sediment core sample varies throughout the length of the core depending on the oxic-anoxic conditions of the sediment. Furthermore, these psychrophilic bacteria, due to their ability to produce extracellular cold active enzymes, might play a key role in the transformation of complex organic compounds.

Sedimentary processes in two different polar periglacial environments: Examples from Schirmacher Oasis and Larsemann Hills, East Antarctica

Abstract Schirmacher Oasis and Bharati Promontory in Larsemann Hills of East Antarctica are currently ice-free coastal areas exposed in physiographically different polar periglacial environments. Schirmacher Oasis is bound by the presence of a vast stretch of ice shelf in the north and the polar ice sheet in the south. It exhibits well-developed patterned ground, abundant till deposition, block-fields, episodic development of curvilinear morainic ridges, extensive outwash plains and erratics. In contrast, the occurrence of such depositional features is sparse on Bharati Promontory, where landmass is directly in contact with the ocean. The sedimentary processes vary in their magnitude owing to different physiographic settings in these two geographically separated locations. Scanning electron microscopy of quartz grains shows subsequent reworking under glaciofluvial environment and final deposition of material in glacially scoured basins. The fluvial action is more pronounced in the Schirmacher Oasis than on Bharati Promontory. The transport of sediment by polar ice is mainly through englacial pathways with a minor contribution from the supraglacial component. Discharge of all sizes of sediments ranging from large boulders to glacially abraded rock-flour in varying proportions takes place at the ice–bedrock interface. Granulometric analysis also shows turbulence of transporting media and reworking of sediments before final deposition.

Transition in Late Quaternary Paleoclimate in Schirmacher Region, East Antarctica as Revealed from Lake Sediments

The lacustrine sediments are one of the best sources to provide information on climate change, specially in peri-glacial climatic region. Schirmacher Oasis, located on the Princess Astrid Coast in Queen Maud Land, is one of the few areas in East Antarctica that provides valuable information on paleoclimate of the region with various depositional features formed due to deglaciation process. This Oasis is dotted with more than 100 lakes of proglacial, land-locked and epi-shelf type. The multi-proxy sedimentological data, generated from the sediment cores from land-locked lakes and grab sample from a proglacial lake, lying in the same drainage line in the central part of Schirmacher region has provided better insight into the paleoclimatic evolution of the region. The immature and chemically unaltered lake sediments have shown restricted drainage pattern. Different phases of warmer and cooler intervals are highlighted by the patterns of fluctuations in different sedimentological and statistical parameters. The dominance of glacial signatures is very clear on the lake sediments as revealed by the surface textures of quartz grains. Physical weathering has mainly controlled the overall sediments and the composition of clay fraction. The clay minerals indicate a gradual shift in the weathering regime and therewith in climate from strongly glacial to fluvioglacial specially around 42 ka. This indicates beginning of warming of the area much before the LGM. But the warm period is not strong enough to alter the overall clay chemistry. Proxy records indicate short-period climatic oscillations during late Quaternary.