Rajesh K. Singh

Circadian periodicity of plasma lipid peroxides and anti-oxidant enzymes in pulmonary tuberculosis.

The circadian periodicity of plasma lipid peroxide levels and activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) were studied in 50 clinically, bacteriologically and radiologically proven fresh cases of pulmonary tuberculosis (age: 21–45 years) and 60 age-matched healthy volunteers with diurnal activity from 06∶00 to about 22∶00 and nocturnal rest. A marked circadian variation in plasma lipid peroxide level was recorded in healthy subjects and pulmonary tuberculosis patients with significant amplitude and acrophase around 16∶21 and 17∶12 respectively. The acrophase tended to be delayed in tuberculosis patients. Furthermore, a statistically significant circadian rhythm was found in SOD, CAT and GPx activities in normal volunteers and pulmonary tuberculopsis patients. SOD and CAT enzyme activity was noted to be maximum at 06∶00 and minimum at 00∶00 in tuberculosis patients. The circadian acrophase for GPx activity was recorded at 16∶15 in normals and around 22∶45 in patients. Moreover, the activity was found to be decreased at all sampling hours during 24-hours sleep-awake period in patients in comparison to healthy counterparts. The MESOR and circadian amplitude also decreased markedly. The decreased activity of measured antioxidant enzymes in pulmonary tuberculosis patients could probably be associated with oxidative stress and/or decreased anti-oxidant defensive mechanism in such patients.

Flow Dynamics of Ions Generated by Laser-Blow-Off of LiF-C Film

The flow dynamics of ejected ions generated by the laser-blow-off of a multicomponent LiF-C thin film deposited on a quartz substrate has been studied using the charge collector probe technique. The spatial distributions of ions were analyzed in different ambient environments ranging from high vacuum to 0.2 mbar of argon pressure and at various laser fluences varying from 2.8 to 26 J/cm2. In vacuum, a double peak structure was observed in the temporal profile of the ion signal at low laser fluence, which slowly transformed into a single peak with increasing laser fluence. The observed plume splitting in vacuum as well as in background gas is interpreted by the formation of ions by two different mechanisms that can produce two different velocity distributions. Present findings at a large collector to target distance, d = 70 mm was found to be consistent with our previously reported optical emission spectroscopic results at a relatively small distance (d = 12 mm).

Effect of gender, age, diet and smoking status on chronomics of circulating plasma lipid components in healthy Indians

BACKGROUNDnCirculating lipid components were studied under near-normal tropical conditions (around Lucknow) in 162 healthy volunteers - mostly medical students, staff members and members of their families (103 males and 59 females; 7 to 75y), subdivided into 4 age groups: A (7-20y; N=42), B (21-40y; N=60), C (41-60y; N=35) and D (61-75y; N=25).nnnMETHODSnBlood samples were collected from each subject every 6h for 24h (4 samples). Plasma was separated and total cholesterol, high-density-lipoprotein (HDL) cholesterol, phospholipids and total lipids were measured spectrophotometrically. Data from each subject were analyzed by cosinor. We examined by multiple-analysis of variance how the MESOR (Midline Estimating Statistic Of Rhythm, a rhythm-adjusted mean) and the circadian amplitude of these variables is affected by gender, age, diet (vegetarian vs. omnivore), and smoking status.nnnRESULTSnIn addition to effects of gender and age, diet and smoking were found to affect the MESOR of circulating plasma lipid components in healthy Indians residing in northern India. Age also affected the circadian amplitude of these variables.nnnCONCLUSIONnThese results indicate the possibility of using non-pharmacological interventions to improve a patients metabolic profile before prescribing medication under near normal tropical conditions. They also add information that may help refine cut-off values in the light of factors shown here to affect blood lipids.

Circadian time structure of circulating plasma lipid peroxides, antioxidant enzymes and other small molecules in peptic ulcers.

BACKGROUNDnThe circadian rhythm, as part of a broad time structure (chronome) of lipid peroxides and antioxidant defense mechanisms may relate to prevention, efficacy and management of preventive and curative chronotherapy.nnnMETHODSnFifty newly diagnosed patients with peptic ulcers, 30-45 years of age, and 60 age-matched clinically healthy volunteers were synchronized for one week with diurnal activity from about 06:00 to about 22:00 and nocturnal rest. Breakfast was served around 08:30, lunch around 13:30 and dinner around 20:30. Drugs known to affect the free-radical systems were not taken. Blood samples were collected at 6-hour intervals for 24h under standardized, presumably 24-hour synchronized conditions. Plasma lipid peroxides, in the form of malondialdehyde (MDA), blood superoxide dismutase (SOD), glutathione peroxide (GPx), glutathione reductase (GR), catalase (CAT) activities, and serum total protein, albumin, ascorbic acid, total serum cholesterol, and HDL-cholesterol concentrations were determined.nnnRESULTSnBy population-mean cosinor analysis, a marked circadian variation was demonstrated for all variables in healthy subjects and in ulcer patients (p<0.001). As compared to controls, patients had a lower MESOR of MDA, SOD, GPx, GR, ascorbic acid, and HDL-C. They also had smaller circadian amplitude of SOD, CAT, GPx, GR, ascorbic acid, T-C, and HDL-C, but larger circadian amplitude of MDA and albumin. As compared to healthy subjects, the circadian acrophase of ulcer patients occurred later for MDA and GR and earlier for GPx.nnnCONCLUSIONnMapping circadian rhythms, important chronome components that include trends with age and extra-circadian components characterizing antioxidants and pro-oxidants, is needed for exploring their putative role as markers in the treatment and management of peptic ulcers.

Diagnostic Value of PCR in Genitourinary Tuberculosis

Genitourinary tuberculosis is a disease of the genitourinary system which includes the entire urinary tract and reproductive system. Genital tuberculosis is an important cause of female infertility, especially in developing nations like India. In the present study, a total of 257 clinical specimens comprising of endometrial biopsy (109), endometrial curetting (42), menstrual blood (8), semen (17), placenta (11) and urine (70) were collected from patients and subjected for PCR, Culture and AFB detection. The endometrial biopsy, endometrial curetting, menstrual blood, semen, placenta, urine showed 30.2, 45.2,12.5, 5.8, 27.2, 31.4xa0%, positivity rate for tuberculosis by PCR, 7.3, 9.5, 25.0, 0, 9, 8.5xa0% by culture and 1.8, 2.3, 0, 0, 0, 2.8xa0% respectively by AFB smear. Being a novel, rapid technique, PCR is the method of choice for rapid diagnosis and management of genitourinary tuberculosis shared with the other concerned tests. This study reveals that genital tuberculosis can occur in any age group, however, the majority of patients were from reproductive age (nearly 75xa0% of them were from 20–45xa0years of age) group.

Usage of Conventional PCR Technology for the Detection of HLA-B27 Allele: A Significant Molecular Marker of Ankylosing Spondylitis

Ankylosing spondylitis is a chronic inflammatory disease that has been linked to the human leukocyte antigen class I allele HLA-B27. More than 90xa0% of patients with ankylosing spondylitis possess the HLA-B27 allele, but only 1xa0% of people with HLA-B27 develop the disease. Ankylosing spondylitis predominately affects young males. The present study was planned to find out the involvement of HLA-B27 specific allele in relation to age and sex in symptomatic suspected patients of ankylosing spondylitis using conventional PCR technology. Forty symptomatic patients of ankylosing spondylitis were included in the present study. SSP–PCR technique was used to detect the HLA-B27 specific allele. This study showed (out of 40 symptomatic suspected cases of ankylosing spondylitis only 12 patients were detected positive with HLA-B27 allele, while remaining 28 were negative) that the positivity rate for ankylosing spondylitis with HLA-B27 allele is low. Furthermore, it was observed that the males above 50xa0years are more prone to develop AS with HLA-B27 specific allele. It could be concluded that the conventional PCR technology is a rapid, sensitive, and confirmatory method for the diagnosis of ankylosing spondylitis.

Study of Laser-Blow-Off Plume Dynamics Using Singular Value Decomposition Technique

Singular value decomposition (SVD) of the fast imaging data of plumes in the laser-blow-off experiment at various background gas pressures is analyzed. It has been observed that structures in the plasma plume are developed not only at the edge but also inside the plume. The principal components based on eigenvectors clearly show the dynamics of plume and its structures both in the longitudinal and lateral directions. SVD analysis is proved to be much clear and better representation than the spectroscopic measurement to show the behavior of expanding plumes, particularly its internal structures in the presence of different ambient environment.

A novel method for fabrication of size-controlled metallic nanoparticles by laser ablation

A simple, yet effective method of controlling the size and size distributions of nanoparticles produced as a result of laser ablation of target material is presented. The method employs the presence of physical boundaries on either sides of the ablation site. In order to demonstrate the potential of the method, experiments have been conducted with copper and titanium as the target materials that are placed in two different liquid media (water and isopropyl alcohol). The ablation of the target material immersed in the liquid medium has been carried out using an Nd:YAG laser. Significant differences in the size and size distributions are observed in the cases of nanoparticles produced with and without confining boundaries. It is seen that for any given liquid medium and the target material, the mean size of the nanoparticles obtained with the boundary-fitted target surface is consistently higher than that achieved in the case of open (flat) targets. The observed trend has been attributed to the plausible role(s) of the confining boundaries in prolonging the thermalisation time of the plasma plume. In order to ascertain that the observed differences in sizes of the nanoparticles produced with and without the presence of the physical barriers are predominantly because of the prolonged thermalisation of the plasma plume and not due to the possible formation of oxide layer, select experiments with gold as the target material in water have also been performed. The experiments also show that, irrespective of the liquid medium, the increase in the mean size of the copper-based nanoparticles due to the presence of physical boundaries is relatively higher than that observed in the case of titanium target material under similar experimental conditions.Time resolved experimental investigation of laser produced plasma-induced shockwaves has been carried out in the presence of confining walls placed along the lateral directions using a Mach Zehnder interferometer in air ambient. Copper was used as target material. The primary and the reflected shock waves and their effects on the evolution of medium density and the plasma density have been studied. The reflected shock wave has been seen to be affecting the shape and density of the plasma plume in the confined geometry. The same experiments were performed with water and isopropyl alcohol as the ambient liquids and the produced nanoparticles were characterised for size and size distribution. Significant differences in the size and size distribution are seen in case of the nanoparticles produced from the ablation of the targets with and without confining boundary. The observed trend has been attributed to the presence of confining boundary and the way it affects the thermalisation time of the plasma plume. The experiments also show the effect of medium density on the mean size of the copper nanoparticles produced.

Chronomics of Circulating Plasma Lipid Peroxides and Antioxidant Enzymes in Renal Stone Formers

The chronome of lipid peroxidation and anti-oxidant defense mechanisms may relate to the efficacy and management of time qualified preventive therapeutic and dietary interventions. One hundred renal stone patients, 20–60xa0years of age, and 50 clinically healthy volunteers, 21–45xa0years, were synchronized for 1xa0week with diurnal activity from 06:00 to 22:00 and nocturnal rest. All subjects took their usual meals three times daily (breakfast around 08:30, lunch around 13:00, and dinner around 20:30) with usual fluid intake. Drugs known to affect free radical system were not taken. Blood samples were collected at 6-h intervals for 24-h under standardized, presumably 24-h synchronized conditions. Determinations included plasma lipid peroxides, in terms of malondialdehyde (MDA) and blood superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR) and catalase (CAT) activities. A marked circadian variation was demonstrated for each studied variable by population-mean cosinor in renal stone patients and healthy participants (pxa0<xa00.001). By comparison to healthy subjects, parameter tests indicate that the stone formers had a higher MESOR of MDA, but a lower MESOR of SOD, GPx, GR and CAT. Furthermore, the patients also differed from the healthy controls in terms of their circadian amplitude and acrophase (tested jointly) of all variables (pxa0<xa00.001). Mapping the broader time structure with multifrequency circadian characteristics of oxidants and anti-oxidants is needed for exploring their role as marker in the treatment and management of urolithiasis.

Numerical modeling of plasma plume evolution against ambient background gas in laser blow off experiments

Two dimensional numerical modelling based on simplified hydrodynamic evolution for an expanding plasma plume (created by laser blow off) against an ambient background gas has been carried out. A comparison with experimental observations shows that these simulations capture most features of the plasma plume expansion. The plume location and other gross features are reproduced as per the experimental observation in quantitative detail. The plume shape evolution and its dependence on the ambient background gas are in good qualitative agreement with the experiment. This suggests that a simplified hydrodynamic expansion model is adequate for the description of plasma plume expansion.