Rajesh Pati

In vitro clonal propagation of bael (Aegle marmelos Corr.) CV. CISH- B1 through enhanced axillary branching

Rapid clonal micropropagation protocol of Aegle marmelos (L.) Corr. cv. CISH-B1 was achieved by nodal stem segment of mature bearing tree. Three centimeter long shoots having one axillary bud excised from 10–15th nodal region of shoots during September gave quick in vitro bud burst (5.33 days) when cultured on MS medium supplemented with BAP, 8.84 μM + IAA 5.7 μM. The maximum number of proliferated shoots (9.0/explant) were obtained on same medium supplemented with BAP 8.84 μM + IAA 5.7 μM. The micro shoots were rooted (100 %) on + IAA 5.7 μM. In vitro rooted plants were acclimatized on autoclaved coconut husk containing plant salt mixture and under shade net house (50 % shade 70–80 % RH). The plants were established in the field after acclimatization. The micropropagated plants were tested for its genetic fidelity using 13 RAPD, 3 ISSR and 2 DAMD primers. Profile obtained by all the three Single Primer Amplification Reaction (SPAR) technique from mother tree and micropropagated plants revealed genetic integrity of micropropagated plants with that of mother tree.

Micropropagation of certain underutilized fruit crops: a review

Abstract Micropropagation is a proven means of producing millions of identical plants under a controlled and aseptic condition, independent of seasonal constraints. It not only provides economy of time and space but also gives greater output and allows further augmentation of elite disease free propagules. India is homeland of many important yet underutilized fruit crops such as Indian gooseberry (Emblica officinalis Gaertn), bael (Aegle marmelos Corr.), jackfruit (Artocarpus hetrophyllus L.), tamarind (Tamarindus indica L.) and jamun or black plum (Syzygium cuminii L. Skeels.). Most of these crops have medicinal value and are suitable for growing under marginal situations. The commercial production of these crops is restricted due to the shortage of desirable planting material. Micropropagation can play an important role in rapidly increasing new cultivars of these fruit crops. This review paper outlines the work done on micropropagation of these fruit crops.