Raman Jeet Singh

Effects of transgenic Bt cotton on soil fertility and biology under field conditions in subtropical inceptisol

Although there is large-scale adoption of Bt cotton by the farmers because of immediate financial gain, there is concern that Bt crops release Bt toxins into the soil environment which reduces soil chemical and biological activities. However, the majorities of such studies were mainly performed under pot experiments, relatively little research has examined the direct and indirect effects of associated cover crop of peanut with fertilization by combined application of organic and inorganic sources of nitrogen under field conditions. We compared soil chemical and biological parameters of Bt cotton with pure crop of peanut to arrive on a valid conclusion. Significantly higher dehydrogenase enzyme activity and KMnO4-N content of soil were observed in Bt cotton with cover crop of peanut over pure Bt cotton followed by pure peanut at all the crop growth stages. However, higher microbial population was maintained by pure peanut over intercropped Bt cotton, but these differences were related to the presence of high amount of KMnO4-N content of soil. By growing cover crop of peanut between Bt cotton rows, bacteria, fungi, and actinomycetes population increased by 60%, 14%, and 10%, respectively, over Bt cotton alone. Bt cotton fertilized by combined application of urea and farm yard manure (FYM) maintained higher dehydrogenase enzyme activity, KMnO4-N content of soil and microbial population over urea alone. Significant positive correlations were observed for dry matter accumulation, dehydrogenase enzyme activity, KMnO4-N content, and microbial population of soil of Bt cotton, which indicates no harmful effects of Bt cotton on soil biological parameters and associated cover crop. Our results suggest that inclusion of cover crop of peanut and FYM in Bt cotton enhanced soil chemical and biological parameters which can mask any negative effect of the Bt toxin on microbial activity and thus on enzymatic activities.

Dry Matter, Nitrogen, Phosphorous, and Potassium Partitioning, Accumulation, and Use Efficiency in Transgenic Cotton-Based Cropping Systems

A better understanding of the fate of nutrients in transgenic cotton (Gossypium hirsutum L.) fields will improve nutrient efficiencies, will optimize crop growth and development, and may help to enhance soil quality. A study was made to evaluate and quantify the effect of cropping system [sole cotton and groundnut (Arachis hypogaea) intercropping with transgenic cotton] and nitrogen (N) management [control (0N), 100% recommended dose of nitrogen (RDN) through urea, substitution of 25% RDN through farmyard manure (FYM), and substitution of 50% RDN through FYM] on dry matter (DM) and nutrient partitioning and accumulation by transgenic cotton and groundnut at New Delhi during 2006–2007. Soil and plant samples were collected and analyzed at 60, 90, and 120 days after sowing and at harvest. Results revealed that intercropping of groundnut with cotton did not significantly affect DM and nutrient partitioning in cotton, but residual soil fertility in terms of potassium permanganate (KMnO4) N showed an improvement in contrast to Olsens P and ammonium acetate (NH4OAc)–exchangeable K over sole cotton. At harvest, of total DM assimilated, leaves constituted 10–20%, stem 50%, and reproductive parts of cotton accounted for the rest. For each kilogram of seed cotton produced, the crop assimilated 61 g of N, of which 23 g was partitioned to harvested seed cotton. Substitution of 25% RDN through FYM, being on par with 100% RDN through urea, recorded greater DM, nutrient uptake in different parts of cotton, agronomic N-use efficiency (9.5 kg seed cotton kg N−1), and apparent N recovery (83.3%) over 50% RDN substitution through FYM and control. The control, being on par with 50% RDN substitution through FYM, recorded significantly greater DM and nutrient uptake by intercropped groundnut over other treatments. Apparent N and potassium (K) balance at the end of study was negative in all treatments; however, the actual change in KMnO4 N was positive in all the treatments except control. Our study suggests that intercropping of groundnut with transgenic cotton and substitution of 25% dose of N through FYM is sustainable in tropical countries.

PRODUCTIVITY AND PROFITABILITY OF THE TRANSGENIC COTTON-WHEAT PRODUCTION SYSTEM THROUGH PEANUT INTERCROPPING AND FYM ADDITION

SUMMARY The cotton–wheat production system (CWPS) occupies an important place in the agricultural economy of several South Asian countries. The instability of the CWPS has increased particularly during the posttransgenic hybrids phase mainly because of these hybrids calling for intensive crop management being cultivated under all situations, especially in resource-poor conditions leading to violent fluctuations during adverse years and thereby affecting the socio-economic status of these developing countries. A study was conducted to evaluate and quantify the effect of the two-tier intercropping of cotton and peanut with the substitution of a 25–50% recommended dose of nitrogen (RDN) of cotton by farmyard manure (FYM) on productivity, profitability and nitrogen economy in the CWPS at New Delhi during 2006–08. To quantify the residual effects of previous crops and their fertility levels, a succeeding crop of wheat was grown with varying rates of nitrogen, viz. 0, 50, 100 and 150 kg ha −1 . Wheat equivalent productivity was significantly more with the inclusion of peanut in the CWPS (21–26%) with a high net return (US

Effects of Transgenic Cotton-Based Cropping Systems and Their Fertility Levels on Succeeding Wheat Crop

288) than a pure stand of cotton in the CWPS. The substitution of 25% RDN of cotton by FYM being on par with no substitution recorded a higher wheat equivalent yield, nitrogen, phosphorus and potassium uptake, net return and nitrogen use efficiencies. Nitrogen economy in wheat was 22 kg ha−1 due to inclusion of peanut in the CWPS and 13 kg ha−1 due to substitution of the 25% RDN of cotton by FYM. The study suggested that for the success of the CWPS in South Asian countries, escalating prices of N fertilizers with environmental issues and the instability of transgenic hybrids can be overcome by using wider rows of cotton by peanut intercrop with the integrated use of both organic and inorganic sources of nitrogen.

Dry Matter Accumulation and Nutrient Uptake by Wheat (Triticumaestivum L.) under Poplar (Populusdeltoides)Based Agroforestry System

Cotton–wheat is the second most important cropping system after rice–wheat in India and Pakistan, and is practiced on about 4.02 mha. By 2010, more than 6 million Indian farmers had adopted transgenic Bt cotton on 9.4 mha—almost 90% of the country’s total cotton area. There is a paucity of information on the effects of intercropping and integrated nitrogen (N)–management practices in transgenic Bt cotton on productivity, nutrient availability, and soil biological properties in the succeeding wheat crop in a cotton–wheat system. A study was made to evaluate and quantify the residual effect of two-tiered intercropping of cotton and groundnut with substitution of 25–50% recommended dose of nitrogen (RDN) of cotton by farmyard manure (FYM) on productivity and soil fertility in a cotton–wheat system at New Delhi during 2006–2008. Wheat following groundnut-intercropped cotton receiving 50% RDN substitution through FYM had significantly 5% greater grain yield than that after sole cotton. Residual soil fertility in terms of organic carbon (C), potassium permanganate (KMnO4)-N, and dehydrogenase activity (14%) showed an improvement under cotton + groundnut–wheat system with substitution of 50% RDN of cotton by FYM. Apparent N balance as well as actual change in KMnO4-N at wheat harvest was negative in most of the treatments, with greater loss (–58.1) noticed under pure stand of the cotton–wheat system with 100% RDN of cotton through urea. The study suggested that inclusion of legume and organic manure in transgenic Bt-cotton–wheat system is a sustainable practice for combating escalating prices of N fertilizers with environmental issues and instability of transgenic hybrids in south Asian countries.

Growth Behaviour of Transgenic Cotton with Peanut Intercropping System Using Modified Fertilization Technique

Wheat (Triticum aestivum L.) being grown with association of boundary plantations of poplar (Populus deltoides M.) has to face competition for water and nutrients uptake. Field experiment was carried to study the dry matter accumulation pattern and nutrients uptake by wheat grown in association with boundary plantations of three- and four-year-old poplar plants under irrigated condition. Dry matter accumulation of wheat declined considerably due to presence of poplar tree line during all the growth stages as compared to pure crop. Maximum reduction in dry matter accumulation in wheat was observed near the tree line (0–3 m) under both three- as well as four-year-old plantation (21.1 and 17.8 per cent under three- and four-year-old trees, resp.) which tapered off beyond that, but synergetic effect caused by existence of trees increased dry matter significantly between 3–6 m distance and 6–9 m distance under both three- as well as four-year-old plantation. Similarly, minimum concentration of nutrients (nitrogen, phosphorus, and potassium) as well as their uptake in wheat plants was observed near the tree line (0–3 m) and increased subsequently with increase in distance from tree line.