Ramanuj Maurya

Diversity analysis in Indian genotypes of linseed (Linum usitatissimum L.) using AFLP markers.

AFLP fingerprinting of 45 Indian genotypes of linseed was carried out to determine the genetic relationship among them. Sixteen primer combinations produced 1142 fragments with 1129 as polymorphic and 13 as monomorphic fragments. Polymorphic fragments varied from 44 (E-ACA/M-CTA) to 94 (E-AGC/M-CAC) with an average of 70.6 fragments per primer combination. The frequency of polymorphism varied from 93.7% to 100% with an average of 98.8% across all the genotypes. The PIC value ranged from 0.19 to 0.31 with an average of 0.23 per primer combination. The primer pair E-AGC/M-CAC showed the maximum PIC value (0.31) followed by E-AGC/M-CAG (0.29), E-AAC/M-CAG (0.26) and E-AGC/M-CTA (0.25). Resolving power (RP) and marker index (MI) varied from 13.73 to 43.50 and 8.81 to 28.91 respectively. The Jaccards similarity coefficient varied from 0.16 to 0.57 with an average of 0.26 ± 0.05. The maximum genetic similarities (57%) were detected between genotypes Him Alsi-1 and Him Alsi-2, followed by Him Alsi-1 and GS41 and GS41 and LC-54. The genotypes R-552, Himani, RKY-14, Meera, Indira Alsi-32 and Suyog were found to be more divergent genotypes. The NJ clustering grouped all the 45 genotypes into three major clusters. In general the genotypes of cluster III had high oil content and those of cluster I had low oil content. At the population level, within population variance was much higher than between populations variance.

Promotion of Berseem Variety Wardan through Frontline Demonstrations in Unnao District of Uttar Pradesh

Berseem (Trifolium alexandrinum) is one of the most important winter forage legumes in India, Pakistan, Turkey, Egypt and countries of Mediterranean region. The crop is reported to be highly self-incompatible in its place of origin but in India it is believed to be self fertile. However, in a recent report, the crop has shown wide diversity for self fertility and population with self compatible and self pollinating, self compatible requiring tripping, Self incompatible types with broad genetic base and self incompatible types with narrow genetic base. In India, it occupies 2 M ha. The merit of the crop lies in its multicut nature (4– 6 cuts), long duration of green fodder availability (November to April), high green fodder yield (85 t/ha), good forage quality (20% crude protein), and digestibility (up to 65%) and high palatability (Anonyms 2009). The yield components in berseem varieties and number of tillers per square meter were major factors related to fodder yield (Beri and International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 7 Number 08 (2018) Journal homepage: http://www.ijcmas.com