Rameshwar Dayal

A flavone glycoside from Dalbergia stipulacea leaves

Abstract A new luteolin 4′-rutinoside and luteolin have been characterized from the leaves of Dalbergia stipulacea .

Stipulin, a prenylated chalcone from Dalbergia stipulacea

Abstract A new diprenylated chalcone stipulin has been characterized from the roots of Dalbergia stipulacea .

Antifungal activity of phytochemicals from Eucalyptus hybrid leaves against some plant pathogenic and wood decay fungi

The in vitro fungitoxicity of the essential oil (EO), crude extracts, dried fractions and ursolic acid (UA) isolated from the abundantly available and underutilised leaves of Eucalyptus hybrid (Eucalyptus camaldulensis × Eucalyptus tereticornis) was assessed against three plant pathogenic fungi Aspergillus niger, Fusarium solani and Ganoderma lucidum causing pathogenesis in seeds, seedlings and trees, respectively, and Trametes versicolor and Pycnoporous sanguineus making wood to decay. The EO, methanol extract and UA demonstrated varied antifungal activity against all the fungi with minimum inhibitory concentrations ranging from 0.5 to 3.0%. The results of the study demonstrate a new chemical utilisation approach of the leaves of Eucalyptus hybrid towards the development of biofungicides in the management of above fungi of common occurrence in Indian forestry.

Chemical Studies of Fatty Oil of Terminalia chebula Seeds Kernels

Abstract The Terminalia chebula fruit kernel oil, constituents of methyl ester derivatives of fatty acids were analyzed using gas chromatography. The results obtained contain saturated (04), as well as unsaturated (02) fatty acids of T. chebula oils. The fatty oil content obtained from the seeds kernels of T. chebula was found to be (37.37-44.91 %) averaging (41.14 %) on moisture free basis. GLC analysis of methyl esters of the fatty acids obtained from the fatty oil after its hydrolysis followed by esterification with methanol/sulphuric acid indicated the presence of eight compounds of which six were identified. The fatty acid composition Table 2 indicated that linoleic acid (39.2 %) is the major constituent of the oil followed by oleic acid (38.5 %), palmitic acid (18.4 %), stearic acid (2.7 %), arachidic acid (0.7 %), behenic acid (0.2 %), and lipids. Due to the presence of a high percentage of valuable analytes in T. chebula, there is an increased importance in its use in pharmaceuticals, as well as its applications in the new formulations for various skin diseases to prevent and cure different infections.

GC/MS analysis of essential oils of needles and twigs of Cephalotaxus harringtonia (Knight ex Forbes) Koch var. harringtonia

Abstract The essential oils of Cephalotaxus harringtonia var. harringtonia needles and twigs were isolated by hydrodistillation. Fifteen compounds of each, representing 86.4% and 89.4% of the oils, respectively, were identified by GC/MS, while their GC/FID analyses indicated the presence of 17 compounds amounting to 86.5% and 89.5% of the identified components in each of the oils. The sesquiterpenes β-caryophyllene (27.9–31.3%), α-humulene (11.4–10.6%), germacrene D (9.5–13.0%), γ-cadinene (8.9–5.5%) and β-elemene (8.3–10.9%) were found to be the major constituents in both the oils.

TLC-DENSITOMETRIC EVALUATION OF THREE MAJOR BIOACTIVE DITERPENE LACTONES IN ANDROGRAPHIS PANICULATA INTERCROPPED WITH MORUS ALBA

Industrially used and endangered listed medicinal plant Andrographis paniculata was cultivated between rows of ten-year-old Morus alba (mulberry) plantation, spaced at 10′ × 10′ under a Randomized Block Design, already existed for sericulture purposes, and evaluated for its performance in terms of content of its three major bioactive diterpene lactones 14-deoxy-11,12-didehydroandrographolide, andrographolide, and neoandrographolide to examine the effect of intercropping on these constituents. Matured plants were harvested and the aerial parts were shade dried, milled, defatted, and extracted with methanol. The extracts were analyzed using HPLTC method validated for linearity, precision, specificity, and accuracy in accordance with the ICH guidelines. Pure lactones required for quantitative determination were isolated from column chromatography of the ethylacetate fraction of the methanol extract obtained from the aerial parts of the plant. Densitometric quantification was performed on Silica gel 60F254 pre-coated glass plates with a mobile phase of chloroform:methanol (7:1, v/v) in a twin trough chamber saturated for 20 min. Statistical analysis revealed significant increase in the contents of these lactones in the A. paniculata when intercropped than grown as sole crops. Thus, suitability of intercropping of A. paniculata in mulberry based cropping system was demonstrated.