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Dive into the research topics where Rameshwar U. Kadam is active.

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Featured researches published by Rameshwar U. Kadam.


Chemistry & Biology | 2008

Inhibition and Dispersion of Pseudomonas aeruginosa Biofilms by Glycopeptide Dendrimers Targeting the Fucose-Specific Lectin LecB

Emma M. V. Johansson; Shanika A. Crusz; Elena Kolomiets; Lieven Buts; Rameshwar U. Kadam; Martina Cacciarini; Kai-Malte Bartels; Stephen P. Diggle; Miguel Cámara; Paul Williams; Remy Loris; Cristina Nativi; Frank Rosenau; Karl-Erich Jaeger; Tamis Darbre; Jean-Louis Reymond

The human pathogenic bacterium Pseudomonas aeruginosa produces a fucose-specific lectin, LecB, implicated in tissue attachment and the formation of biofilms. To investigate if LecB inhibition disrupts these processes, high-affinity ligands were obtained by screening two 15,536-member combinatorial libraries of multivalent fucosyl-peptide dendrimers. The most potent LecB-ligands identified were dendrimers FD2 (C-Fuc-LysProLeu)(4)(LysPheLysIle)(2)LysHisIleNH(2) (IC(50) = 0.14 microM by ELLA) and PA8 (OFuc-LysAlaAsp)(4)(LysSerGlyAla)(2)LysHisIleNH(2) (IC(50) = 0.11 microM by ELLA). Dendrimer FD2 led to complete inhibition of P. aeruginosa biofilm formation (IC(50) approximately 10 microM) and induced complete dispersion of established biofilms in the wild-type strain and in several clinical P. aeruginosa isolates. These experiments suggest that LecB inhibition by high-affinity multivalent ligands could represent a therapeutic approach against P. aeruginosa infections by inhibition of biofilm formation and dispersion of established biofilms.


Angewandte Chemie | 2011

A Glycopeptide Dendrimer Inhibitor of the Galactose-Specific Lectin Leca and of Pseudomonas Aeruginosa Biofilms.

Rameshwar U. Kadam; Myriam Bergmann; Matthew Hurley; Divita Garg; Martina Cacciarini; Magdalena A. Swiderska; Cristina Nativi; Michael Sattler; Alan Smyth; Paul Williams; Miguel Cámara; Achim Stocker; Tamis Darbre; Jean-Louis Reymond

Biofilm inhibition is achieved with a phenylgalactosyl peptide dendrimer (see picture) that binds to the galactose-specific lectin LecA of P. aeruginosa. The multivalency of the ligands is critical for biofilm inhibition, although the nature of the linker between the peptide dendrimer and the galactose can provide additional contacts to the lectin and also has an effect on the interaction.


ChemMedChem | 2009

Glycopeptide Dendrimers with High Affinity for the Fucose‐Binding Lectin LecB from Pseudomonas aeruginosa

Elena Kolomiets; Magdalena A. Swiderska; Rameshwar U. Kadam; Emma M. V. Johansson; Karl-Erich Jaeger; Tamis Darbre; Jean-Louis Reymond

Dendritic antibacterial agents: Glycopeptide dendrimer biofilm inhibitors were synthesized combinatorially and optimized for binding to the fucose‐specific lectin LecB, which has high affinity of fucose. These dendritic ligands are potential antibacterial agents against Pseudomonas aeruginosa, an antibiotic resistant human pathogen.


ACS Chemical Biology | 2013

CH−π “T-Shape” Interaction with Histidine Explains Binding of Aromatic Galactosides to Pseudomonas aeruginosa Lectin LecA

Rameshwar U. Kadam; Divita Garg; Julian Schwartz; Ricardo Visini; Michael Sattler; Achim Stocker; Tamis Darbre; Jean-Louis Reymond

The galactose specific lectin LecA mediates biofilm formation in the opportunistic pathogen P. aeruginosa . The interaction between LecA and aromatic β-galactoside biofilm inhibitors involves an intermolecular CH-π T-shape interaction between C(ε1)-H of residue His50 in LecA and the aromatic ring of the galactoside aglycone. The generality of this interaction was tested in a diverse family of β-galactosides. LecA binding to aromatic β-galactosides (KD ∼ 8 μM) was consistently stronger than to aliphatic β-galactosides (KD ∼ 36 μM). The CH-π interaction was observed in the X-ray crystal structures of six different LecA complexes, with shorter than the van der Waals distances indicating productive binding. Related XH/cation/π-π interactions involving other residues were identified in complexes of aromatic glycosides with a variety of carbohydrate binding proteins such as concanavalin A. Exploiting such interactions might be generally useful in drug design against these targets.


MedChemComm | 2012

Membrane disrupting antimicrobial peptide dendrimers with multiple amino termini

Michaela Stach; Noélie Maillard; Rameshwar U. Kadam; David Kalbermatter; Marcel Meury; Malcolm G. P. Page; Dimitrios Fotiadis; Tamis Darbre; Jean-Louis Reymond

Antimicrobial peptide dendrimer H1 Leu8(Lys-Leu)4(Lys-Phe)2Lys-LysNH2 (Lys = branching lysine) was identified by screening a 6750-membered combinatorial library by the bead-diffusion assay. Sequence variations also revealed dendrimer bH1 Leu8(Dap-Leu)4(Dap-Phe)2Dap-LysNH2 (Dap = branching 2,3-diaminopropanoic acid) as a more potent analog. H1 and bH1 showed good antimicrobial activities mediated by membrane disruption (MIC = 2–4 μg mL−1 on Bacillus subtilis and Escherichia coli) but low hemolytic activity (MHC = 310 μg mL−1 respectively >2000 μg mL−1).


Chemistry: A European Journal | 2013

Structure-Based Optimization of the Terminal Tripeptide in Glycopeptide Dendrimer Inhibitors of Pseudomonas aeruginosa Biofilms Targeting LecA

Rameshwar U. Kadam; Myriam Bergmann; Divita Garg; Gabriele Gabrieli; Achim Stocker; Tamis Darbre; Jean-Louis Reymond

The galactopeptide dendrimer GalAG2 ((β-Gal-OC6H4CO-Lys-Pro-Leu)4(Lys-Phe-Lys-Ile)2Lys-His-Ile-NH2) binds strongly to the Pseudomonas aeruginosa (PA) lectin LecA, and it inhibits PA biofilms, as well as disperses already established ones. By starting with the crystal structure of the terminal tripeptide moiety GalA-KPL in complex with LecA, a computational mutagenesis study was carried out on the galactotripeptide to optimize the peptide-lectin interactions. 25 mutants were experimentally evaluated by a hemagglutination inhibition assay, 17 by isothermal titration calorimetry, and 3 by X-ray crystallography. Two of these tripeptides, GalA-KPY (dissociation constant (K(D))=2.7 μM) and GalA-KRL (K(D)=2.7 μM), are among the most potent monovalent LecA ligands reported to date. Dendrimers based on these tripeptide ligands showed improved PA biofilm inhibition and dispersal compared to those of GalAG2, particularly G2KPY ((β-Gal-OC6H4CO-Lys-Pro-Tyr)4(Lys-Phe-Lys-Ile)2Lys-His-Ile-NH2). The possibility to retain and even improve the biofilm inhibition in several analogues of GalAG2 suggests that it should be possible to fine-tune this dendrimer towards therapeutic use by adjusting the pharmacokinetic parameters in addition to the biofilm inhibition through amino acid substitutions.


ChemBioChem | 2010

Structure and Binding of Peptide-Dendrimer Ligands to Vitamin B12

Nicolas A. Uhlich; Antonino Natalello; Rameshwar U. Kadam; Silvia Maria Doglia; Jean-Louis Reymond; Tamis Darbre

The third‐generation peptide‐dendrimer B1 (AcES)8(BEA)4(K‐Amb‐Y)2BCD‐NH2 (B=branching (S)‐2,3‐diaminopropanoic acid, K=branching lysine, Amb=4‐aminomethyl‐benzoic acid) is the first synthetic model for cobalamin‐binding proteins and binds cobalamin strongly (Ka=5.0×106 M−1) and rapidly (k2=346 M−1 s−1) by coordination of cobalt to the cysteine residue at the dendrimer core. A structure–activity relationship study is reported concerning the role of negative charges in binding. Substituting glutamates (E) for glutamines (Q) in the outer branches of B1 to form N3 (AcQS)8(BQA)4(B‐Amb‐Y)2BCD‐NH2 leads to stronger (Ka=12.0×106 M−1) but slower (k2=67 M−1 s−1) cobalamin binding. CD and FTIR spectra show that the dendrimers and their cobalamin complexes exist as random‐coil structures without aggregation in solution. The hydrodynamic radii of the dendrimers determined by diffusion NMR either remains constant or slightly decreases upon binding to cobalamin; this indicates the formation of compact, presumably hydrophobically collapsed complexes.


Chemical Biology & Drug Design | 2008

Structure Function Analysis of Leishmania Sirtuin: An Ensemble of In Silico and Biochemical Studies

Rameshwar U. Kadam; Joana Tavares; V.M. Kiran; Anabela Cordeiro; Ali Ouaissi; Nilanjan Roy

Novel anti‐leishmanial target LmSir2 has few subtle but prudent structural differences in ligand binding and catalytic domain as compared to its human counterpart. In silico screening and validation followed by in vitro deacetylation and cell killing assays described herein give a proof of concept for development of strategies exploiting such minor differences for screening libraries of small molecules to identify selective inhibitors.


Chemical Communications | 2011

Expanding the accessible chemical space by solid phase synthesis of bicyclic homodetic peptides

Marco Bartoloni; Rameshwar U. Kadam; Julian Schwartz; Julien Furrer; Tamis Darbre; Jean-Louis Reymond

Norbornapeptides (bicyclo[2.2.1]heptapeptides) and related bicyclic homodetic peptides were prepared by solid-phase peptide synthesis using an orthogonal protection scheme. These conformationally rigid peptides cover an almost pristine area of peptide topological space and adopt globular shapes similar to those of short α-helical peptides.


Chemical Biology & Drug Design | 2007

Selective Mapping of Chemical Space for Pseudomonas aeruginosa Deacetylase LpxC Inhibitory Potential

Rameshwar U. Kadam; Divita Garg; Nilanjan Roy

UDP‐3‐O‐[R‐3‐hydroxymyristoyl]‐GlcNAc deacetylase enzyme of Pseudomonas aeruginosa is an interesting target for development of anti‐infective drugs against this gram‐negative bacterium. Many segregated studies analyzing the P. aeruginosa UDP‐3‐O‐[R‐3‐hydroxymyristoyl]‐GlcNAc deacetylase and its inhibitors have been reported in the recent past. In the present study, an attempt has been made to integrate this knowledge for the development of an effective multilayer screening approach. Eventually, an extensive chemical space was screened to filter out three potential P. aeruginosa UDP‐3‐O‐[R‐3‐hydroxymyristoyl]‐GlcNAc deacetylase inhibitors.

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Divita Garg

Center for Integrated Protein Science Munich

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Miguel Cámara

University of Nottingham

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Paul Williams

University of Nottingham

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