Randall J. Harris

Root Coverage With a Connective Tissue With Partial Thickness Double Pedicle Graft and an Acellular Dermal Matrix Graft: A Clinical and Histological Evaluation of a Case Report

Root coverage can be achieved by many techniques. In this case report a patient was treated with a connective tissue with partial thickness double pedicle graft, which resulted in complete root coverage. However, 6 days postsurgery the patient developed a bleeding problem from the palatal donor area. For this reason, a unique approach was used to treat the next area needing root coverage. The procedure combined an acellular dermal matrix and a coronally positioned pedicle. The procedure was used to treat 3 defects. Complete root coverage was obtained on 2 of the 3 defects. The third was covered to within 1 mm of the cemento-enamel junction (CEJ). Histology showed similar results with a connective tissue graft and an acellular dermal matrix. Verhoeffs staining demonstrated that the acellular dermal matrix was incorporated into the gingival tissue. This case report demonstrated that acceptable results can be obtained with the connective tissue with partial thickness double pedicle graft and the acellular dermal matrix combined with a coronally positioned pedicle, both clinically and histologically.

Role of Maternal Serum Alpha-Fetoprotein and Ultrasonography in Contemporary Detection of Spina Bifida.

OBJECTIVE Midtrimester maternal serum alpha-fetoprotein (MSAFP) and sonographic evaluation have been used to screen for spina bifida. With the increased uptake of cell-free DNA (cfDNA) and first trimester screening, MSAFP levels may no longer be obtained routinely. Our aim was to evaluate a pediatric neurosurgical referral center database of spina bifida cases to determine the antenatal detection rate and means of diagnosis. STUDY DESIGN Nested case series of all spina bifida cases referred postnatally from 2007 to 2013. Data were abstracted from the maternal record and rates of antenatal detection with MSAFP and sonographic screening were determined. RESULTS Of the 105 postnatally referred cases, 11.4% (12/105) were not identified until delivery. Overall, 39% of the cases had MSAFP screening. The odds ratio for sonogram-based detection of spina bifida was 4.9 (95% confidence interval, 2-11.9). Of the neonatally detected cases, 100% had prenatal care and 91.6% (11 of the 12 cases) had documented sonography. CONCLUSION We have found that 11.4% of the spina bifida cases were not detected before delivery. Nine out of the 12 cases of antenatally missed spina bifida were not screened using MSAFP. Our findings support the approach of midtrimester MSAFP screening combined with sonographic evaluation. We speculate that prenatal screening with MSAFP is underutilized.