Raphael Emuebie Okonji

Prophylactic and Curative Assessment of Essentiale Forte® On Carbon Tetrachloride-Induced Liver Damage in Wistar Rats

1 Department of Physiological Sciences, Obafemi Awolowo University, Ile-Ife, Nigeria; 2 Department of Medical Pharmacology and Therapeutics, Obafemi Awolowo University, Ile-Ife, Nigeria; 3 Department of Medicine, College of Medicine, Ekiti State University, Ado-Ekiti, Nigeria; 4 Department of Biochemistry, Obafemi Awolowo University, Ile-Ife, Nigeria; 5 Department of Morbid Anatomy and Forensic Medicine, Obafemi Awolowo University, Ile-Ife, Nigeria

Comparative Study of Thiaminase in Headfoot and Hepatopancreas of Limicolaria flammea (Müller, 1774)

Aims: This study aimed at determining the level of thiaminase in the Limicolaria flammea with a view to ascertaining the nutritional and health implications of its consumption. Methodology: Proximate analyses of two tissues, headfoot and hepatopancreas, were investigated to assess their nutritional qualities. The thiaminases in the tissues were purified by chromatographic separations using DEAE-Sephacel ion - exchange and Biogel P-100 columns. Results: The Michealis Menten constant obtained for hepatopancreas thiaminase was 0.83 mM and 0.13 mM for thiamine and aniline respectively; for the headfoot thiaminase, 1.06 mM and 0.16 mM was obtained for thiamine and aniline respectively. An optimum pH of 8.5 was obtained for thiaminase in the two tissues. Temperature optimum of 65°C and 70°C was obtained for the headfoot and hepatopancreas enzymes respectively. The amino acids and glutathione activated the enzyme from hepatopancreas, while the headfoot enzyme was significantly inhibited except proline which showed very high activation. The cations: NH 4 + , Hg