Rasmina Halis

Effects of plant growth regulators, carbon sources and pH values on callus induction in Aquilaria malaccensis leaf explants and characteristics of the resultant calli

The endangered tropical tree, Aquilaria malaccensis, produces agarwood for use in fragrance and medicines. Efforts are currently underway to produce valuable agarwood compoundsn tissue culture. The purpose of this study was to develop an optimal growth medium, specifically, the best hormone combination for callus suspension culture. Using nursery-grown A. malaccensis, sterilized leaf explants were first incubated on basic Murashige and Skoog (MS) gel medium containing 15g/L sucrose and at pH 5.7. Different auxin types including 1-naphthaleneacetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D), and indole-3-butyric acid (IBA), were tested at various concentrations (0.55, 1.1 and 1.65 μM) using the basic medium. Leaf explants were incubated for 30 days in the dark. Callus induced by 1.1 μM NAA had the highest biomass dry weight (DW) of 17.3 mg; however the callus was of a compact type. This auxin concentration was then combined with either 6-benzylaminopurine (BAP) or kinetin at 0.55, 1.1, 2.2 or 3.3 μM to induce growth of friable callus. The 1.1μM NAA + 2.2μM BAP combination produced friable callus with the highest biomass (93.3mg DW). When testing the different carbon sources and pHs, sucrose at 15g/L and pH at 5.7 yielded highest biomasses at 87.7mg and 83mg DW, respectively. Microscopic observations revealed the arrangement of the friable cells as loosely packed with relatively large cells, while for the compact callus, the cells were small and densely packed. We concluded that MS medium containing 15 g/L sucrose, 1.1 μM NAA + 2.2 μM BAP hormone combination, and a pH of 5.7 was highly effective for inducing friable callus from leaf explants of A. malaccensis for the purpose of establishing cell suspension culture.

Chemical, physico-mechanical properties and biological durability of rubberwood particleboards after post heat-treatment in palm oil

Abstract The chemical properties, dimensional stability, mechanical strength and termite resistance of urea formaldehyde (UF) bond rubberwood (RW) particleboard (PB) were assessed after a two-step oil heat treatment (OHT). The PB was immersed in palm oil before heating to 180, 200, and 220°C in a laboratory oven for 2 h. Anti-swelling efficiency (ASE) and water repellency efficiency (WRE) as well as bending (MOE, MOR) and internal bonding strength (IB) were determined. Resistance against a subterranean termite, Coptotermes curvignathus Holmgren, was tested. The degradation of hemicelluloses and cellulose, that are mainly responsible for wood wetting processes, was confirmed by Fourier transform infrared (FTIR) spectra. Formation of an elevated cross-linking density in lignin also contributed to the dimensional stability, where 93.6% ASE and 46.3% WRE were achieved in the samples treated at 220°C. Mechanical properties of treated samples were inferior to the control samples due to hemicelluloses degradation and breakage of the UF bonding network. A significant improvement in termite resistance has been found in the treated samples.