Raúl Luis García Aranda

Comparación in vitro de la actividad antimicrobiana de AhPlus, RSA y Ledermix contra Enterococcus faecalis

Aim: In vitro assessment of antimicrobial activity sustained by two root canal sealers: RSA ® , AH Plus ® as well as Ledermix ® paste upon Enterococcus faecalis using three different techniques. Method: Direct contact test (DCT). Sealers were placed on an acrylic surface. A E. faecalis suspension was inoculated and left in a microtube with 1 mL of BHI broth. Logarithmic dilutions were conducted spreading them in blood agar plates so as to quantify CFUs (colony forming units). Dilution test (DT). Sealers and paste were placed in a plastic cylinder. Bacteriae were inoculated in the culture broth and the same quantification procedure was undertaken. Agar dilution test (ADT). On a blood agar plate three wells were manufactured: they were filled with both cements. On the Ledermix paste surface a E. faecalis suspension was inoculated so as to assess growth inhibition areas. Results: In the Direct contact test, Ledermix paste showed higher antimicrobial activity percentage. Neither of both cements nor the paste presented antimicrobial activity in dilution and Agar dilution test. In the Agar dilution test, AH Plus sealer and Ledermix paste exhibited a hemolysis halo in the blood agar plates. Conclusions: Direct contact test technique was considered the most appropriate to assess antimicrobial effects of cements. RESUMEN

Evaluación de la citotoxicidad de distintos cementos selladores endodónticos en cultivos de fibroblastos gingivales

El objetivo de esta investigacion fue evaluar in vitro la respuestade fi broblastos gingivales contra diferentes selladores endodonticosmediante cultivos celulares, en un lapso de 96 horas. Los resultadosobtenidos a intervalos de tiempo de 0, 1, 2, 3, 6, 24, 48, 72 y96 horas fueron utilizados para determinar la citotoxicidad de losselladores. Cultivos de fi broblastos gingivales sin ningun sellador ycon Sealapex fueron utilizados como controles positivos y negativosrespectivamente. Los resultados fueron comparados con los controlesnegativos y analizados estadisticamente por medio de la pruebat Dunnett (p ≤ 0.05). Los cementos selladores investigados fueron:ProRoot MTA gris y blanco CPM, MTA Angelus, Sealapex y Gutta-Flow. Los resultados demostraron que a pesar de que el ProRootMTA (gris y blanco) MTA Angelus, CPM y GuttaFlow demostrarontener un potencial citotoxico menor que el Sealapex, no se encontrarondiferencias estadisticas signifi cativas.