Raymond A. Brown

Reagic serum fractions obtained by the electrophoresis-convection method

Abstract 1.1. The fractionation of serums from allergic individuals has been accomplished by electrophoresis-convection methods. 2.2. Prausnitz-Kustner tests on the resulting fractions indicated that reagic activity was closely associated with the α- and β-globulins, particularly in simple sensitivities and that activity occurred in all the globulins in multisensitivity.

Significance of a Single-hit Event in the Initiation of Antibody Response

THE origin of the physico-chemical and immuno-chemical heterogeneity of immunoglobulin molecules has been a perplexing problem for many years. Recently, evidence has been accumulating which points to variability in the expression of antigen-stimulable progenitor cells as the cause of immunoglobulin heterogeneity. Whether or not this is the case—or whether, for example, the variability may result from non-uniformity of polypeptide chain construction by polysomes—can only be decided when methods are available for analysis of immuno-globulins at various times during response by the progeny of a single precursor cell. Before such clonal investigations can be attempted, it is obviously necessary to have some clear notion about the number and frequency of these precursor cells. This communication presents the results of experiments designed to enumerate such cells in spleen cell suspensions from non-primed and primed mice of two different hybrid strains of various age groups.

The theory of electrophoresis-convection

Abstract The theory of transport in the electrophoresis-channel is developed in the quasi-stationary approximation on the basis of the equations of hydrodynamics and the equations of forced diffusion of ion constituents under the influence of an applied electric field. Times of transport of proteins through the channel and separation factors for representative protein mixtures are estimated by means of the theory.