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Dive into the research topics where Reg Gorczynski is active.

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Featured researches published by Reg Gorczynski.


Transplantation | 2007

Mice lacking CD200R1 show absence of suppression of lipopolysaccharide-induced tumor necrosis factor-alpha and mixed leukocyte culture responses by CD200.

Ivo Boudakov; Jian Liu; Na Fan; Pelin Gulay; Karrie Wong; Reg Gorczynski

Background. CD200:CD200R interactions deliver immunoregulatory signals. A family of CD200Rs (CD200R1-5) has been described, and engagement of CD200R1 by its ligand CD200 suppresses LPS-induced macrophage cytokine production, decreases alloimmune responses in vivo and in vitro, and suppresses collagen-induced arthritis. Methods. We generated C57BL/6 mice lacking the genomic exons encoding the extracellular domains of the CD200R1 molecule using transformation of ES cells and explored cell subtypes and immune responses in these mice. Results. Myeloid cells/splenocytes from CD200R1−/− mice were not stained in FACS by anti-CD200R1 mAb. Stimulation of splenic tumor necrosis factor-&agr; production by lipopolysaccharide was enhanced relative to control (+/+) mice and was not suppressed by addition of exogenous CD200Fc. Modulation of alloreactivity in mixed leukocyte cultures by CD200Fc depended upon CD200R1+ stimulatory cells, although maximal immunoregulation by CD200Fc occurred only when CD200R1+ T responder cells also were used. CD200Fc failed to suppress graft rejection in CD200R1−/− mice. Conclusion. CD200:CD200R1 plays an immunoregulatory role in vivo.


Journal of Immunology | 2008

An Interaction between CD200 and Monoclonal Antibody Agonists to CD200R2 in Development of Dendritic Cells That Preferentially Induce Populations of CD4+CD25+ T Regulatory Cells

Reg Gorczynski; Ismat Khatri; Lydia Lee; Ivo Boudakov

In previous studies we reported that while interaction between the relatively ubiquitously expressed molecule CD200 and one of its receptors, CD200R1, resulted in direct suppression of alloreactivity, engagement of alternate receptors led instead to altered differentiation of dendritic cells (DCs) from marrow precursors, which could in turn foster development of Foxp3+ regulatory T cells. We have explored this effect of engagement of alternate receptors by using a monoclonal agonist Ab to CD200R2 and investigating expression of TLRs on DCs induced in vivo and in vitro after CD200 stimulation in mice in which the gene encoding CD200R1 was deleted. CD200 stimulation was achieved by using either a soluble form of CD200 (CD200Fc) or overexpression of CD200 as a doxycycline-inducible transgene. Although broadly similar effects were seen, consistent with the hypothesis that triggering of CD200R2 does produce DCs with a characteristic TLR repertoire, there are subtle differences in suppression of alloreactivity achieved by CD200 delivered in these two manners, which is consistent with a complexity of CD200:CD200R engagement not previously appreciated.


Oncotarget | 2018

CD200fc enhances anti-tumoral immune response and inhibits visceral metastasis of breast carcinoma

Nuray Erin; Gamze Tanriover; Anna Curry; Muhlis Akman; Özlem Duymuş; Reg Gorczynski

CD200 is a widely expressed cell surface glycoprotein that inhibits excessive inflammation in autoimmunity, transplantation, and viral infections. We previously observed that visceral metastasis of highly aggressive and inflammatory 4THM breast carcinoma cells was markedly decreased in CD200 transgenic mice. The goal of this study was to determine whether exogenous exposure to CD200fc mimics the effects of endogenously over expressed CD200. Female BALB/c mice were injected with CD200fc two times a week for five times. Injection was started two days after orthotopic injection of 4THM cells. Tumor infiltrating Gr1+Cd11b+ cells were decreased while CD8+ cells were increased in CD200fc-treated animals. CD200fc injection significantly decreased lung and liver metastasis and the growth of primary tumors. CD200fc injection enhanced the tumor-induced IFN-g response while suppressing the IL-10 response. We observed excessive basal IL-6 secretion in MLC which was significantly decreased in CD200fc treated mice 12 days after injection of 4TM cells. These results are in accord with previous data from CD200 transgenic mice, and demonstrate for the first time that CD200 analogues might have therapeutic potential in the treatment of aggressive breast carcinoma which induces excessive systemic inflammation.


Journal of Surgical Research | 2008

Peptides of CD200 Modulate LPS-Induced TNF-α Induction and Mortality In Vivo

Reg Gorczynski; Ivo Boudakov; Ismat Khatri


Clinical Immunology | 2007

Bone-Marrow Derived DCs From CD200R1KO Mice Stimulated with the Ligand CD200Fc Preferentially Induce Populations of CD4+CD25+Treg

Reg Gorczynski; Ivo Boudakov


Journal of Immunology | 2014

Immunization of CD200R1KO mice post surgical resection of EMT6 breast cancer leads to long-term (>1year) cure of metastatic tumor growth (TUM7P.921)

Reg Gorczynski; Zhiqi Chen; Ismat Khatri; Anna Podnos


Journal of Immunology | 2014

Increased lung/liver metastasis of human breast cancer cells in NOD.SCIDIL2rγ-/- mice after treatment with soluble CD200Fc (TUM7P.922)

Reg Gorczynski; Zhiqi Chen; Ismat Khatri; Anna Podnos; Olha Kos


Clinical Immunology | 2010

Role of Host and Tumor CD200 Expression in Control of Local and Distant (metastatic) Growth of Breast Cancer in Mice

Reg Gorczynski; Nuray Erin; Kai Yu; Ismat Khatri


Clinical Immunology | 2010

A Role for and Mechanism of Action of a Novel Molecule, CD200Fc(Gly)6TGFβ, in Suppression of Transplant Rejection

Reg Gorczynski; Zhiqi Chen; Saaiis Shivagnahnam; Adriana Taseva; Karrie Wong; Kai Yu; Ismat Khatri


Clinical Immunology | 2009

F.46. Continued Expression of the CD200 Transgene in Transgenic Mice is Not Essential for Prolonged Graft Survival

Reg Gorczynski; Ivo Boudakov; William He; Gary Yu

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Ismat Khatri

University Health Network

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Ivo Boudakov

University Health Network

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Kai Yu

University Health Network

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Karrie Wong

University Health Network

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Zhiqi Chen

University Health Network

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Anna Podnos

University Health Network

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Adriana Taseva

University Health Network

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