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Dive into the research topics where Reinhold Förster is active.

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Featured researches published by Reinhold Förster.


Nature | 1999

Two subsets of memory T lymphocytes with distinct homing potentials and effector functions.

Federica Sallusto; Danielle Lenig; Reinhold Förster; Martin Lipp; Antonio Lanzavecchia

Naive T lymphocytes travel to T-cell areas of secondary lymphoid organs in search of antigen presented by dendritic cells. Once activated, they proliferate vigorously, generating effector cells that can migrate to B-cell areas or to inflamed tissues. A fraction of primed T lymphocytes persists as circulating memory cells that can confer protection and give, upon secondary challenge, a qualitatively different and quantitatively enhanced response. The nature of the cells that mediate the different facets of immunological memory remains unresolved. Here we show that expression of CCR7, a chemokine receptor that controls homing to secondary lymphoid organs, divides human memory T cells into two functionally distinct subsets. CCR7- memory cells express receptors for migration to inflamed tissues and display immediate effector function. In contrast, CCR7+ memory cells express lymph-node homing receptors and lack immediate effector function, but efficiently stimulate dendritic cells and differentiate into CCR7- effector cells upon secondary stimulation. The CCR7+ and CCR7- T cells, which we have named central memory (TCM) and effector memory (TEM), differentiate in a step-wise fashion from naive T cells, persist for years after immunization and allow a division of labour in the memory response.


Cell | 1999

CCR7 Coordinates the Primary Immune Response by Establishing Functional Microenvironments in Secondary Lymphoid Organs

Reinhold Förster; Andreas Schubel; Dagmar Breitfeld; Elisabeth Kremmer; Ingrid Renner-Müller; Eckhard Wolf; Martin Lipp

The proper function of immune surveillance requires well-coordinated mechanisms in order to guide the patrolling immune cells through peripheral tissues and into secondary lymphoid organs. Analyzing gene-targeted mice, we identified the chemokine receptor CCR7 as an important organizer of the primary immune response. CCR7-deficient mice show severely delayed kinetics regarding the antibody response and lack contact sensitivity and delayed type hypersensitivity reactions. Due to the impaired migration of lymphocytes, these animals reveal profound morphological alterations in all secondary lymphoid organs. Upon activation, mature skin dendritic cells fail to migrate into the draining lymph nodes. Thus, in order to bring together lymphocytes and dendritic cells to form the characteristic microarchitecture of secondary lymphoid organs, CCR7 is required to rapidly initiate an adoptive immune response.


Nature | 1998

CD40 ligand on activated platelets triggers an inflammatory reaction of endothelial cells

Volker Henn; Joseph R. Slupsky; Michael Gräfe; Ioannis Anagnostopoulos; Reinhold Förster; Gert Müller-Berghaus; Richard A. Kroczek

CD40 ligand (CD40L, CD154), a transmembrane protein structurally related to the cytokine TNF-α, was originally identified on stimulated CD4+ T cells, and later on stimulated mast cells and basophils. Interaction of CD40L on T cells with CD40 on B cells is of paramount importance for the development and function of the humoral immune system. CD40 is not only constitutively present on B cells, but it is also found on monocytes, macrophages and endothelial cells, suggesting that CD40L has a broader function in vivo. We now report that platelets express CD40L within seconds of activation in vitro and in the process of thrombus formation in vivo. Like TNF-α and interleukin-1, CD40L on platelets induces endothelial cells to secrete chemokines and to express adhesion molecules, thereby generating signals for the recruitment and extravasation of leukocytes at the site of injury. Our results indicate that platelets are not only involved in haemostasis but that they also directly initiate an inflammatory response of the vessel wall.


Cell | 1996

A PUTATIVE CHEMOKINE RECEPTOR, BLR1, DIRECTS B CELL MIGRATION TO DEFINED LYMPHOID ORGANS AND SPECIFIC ANATOMIC COMPARTMENTS OF THE SPLEEN

Reinhold Förster; Anita E Mattis; Elisabeth Kremmer; Eckhard Wolf; G. Brem; Martin Lipp

We describe the phenotype of gene-targeted mice lacking the putative chemokine receptor BLR1. In normal mice, this receptor is expressed on mature B cells and a subpopulation of T helper cells. Blr1 mutant mice lack inguinal lymph nodes and possess no or only a few phenotypically abnormal Peyers patches. The migration of lymphocytes into splenic follicles is severely impaired, resulting in morphologically altered primary lymphoid follicles. Furthermore, activated B cells fail to migrate from the T cell-rich zone into B cell follicles of the spleen, and despite high numbers of germinal center founder cells, no functional germinal centers develop in this organ. Our results identify the putative chemokine receptor BLR1 as the first G protein-coupled receptor involved in B cell migration and localization of these cells within specific anatomic compartments.


Nature | 2000

A chemokine-driven positive feedback loop organizes lymphoid follicles.

K. Mark Ansel; Vu N. Ngo; Paul L. Hyman; Sanjiv A. Luther; Reinhold Förster; Jonathon D. Sedgwick; Jeffrey L. Browning; Martin Lipp; Jason G. Cyster

Lymphoid follicles are B-cell-rich compartments of lymphoid organs that function as sites of B-cell antigen encounter and differentiation. CXC chemokine receptor-5 (CXCR5) is required for B-cell migration to splenic follicles, but the requirements for homing to B-cell areas in lymph nodes remain to be defined. Here we show that lymph nodes contain two types of B-cell-rich compartment: follicles containing follicular dendritic cells, and areas lacking such cells. Using gene-targeted mice, we establish that B-lymphocyte chemoattractant (BLC/BCA1) and its receptor, CXCR5, are needed for B-cell homing to follicles in lymph nodes as well as in spleen. We also find that BLC is required for the development of most lymph nodes and Peyers patches. In addition to mediating chemoattraction, BLC induces B cells to upregulate membrane lymphotoxin α1β2, a cytokine that promotes follicular dendritic cell development and BLC expression, establishing a positive feedback loop that is likely to be important in follicle development and homeostasis. In germinal centres the feedback loop is overridden, with B-cell lymphotoxin α1β2 expression being induced by a mechanism independent of BLC.


Nature | 2008

Rapid leukocyte migration by integrin-independent flowing and squeezing

Tim Lämmermann; Bernhard L. Bader; Susan J. Monkley; Tim Worbs; Roland Wedlich-Söldner; Karin Hirsch; Markus Keller; Reinhold Förster; David R. Critchley; Reinhard Fässler; Michael Sixt

All metazoan cells carry transmembrane receptors of the integrin family, which couple the contractile force of the actomyosin cytoskeleton to the extracellular environment. In agreement with this principle, rapidly migrating leukocytes use integrin-mediated adhesion when moving over two-dimensional surfaces. As migration on two-dimensional substrates naturally overemphasizes the role of adhesion, the contribution of integrins during three-dimensional movement of leukocytes within tissues has remained controversial. We studied the interplay between adhesive, contractile and protrusive forces during interstitial leukocyte chemotaxis in vivo and in vitro. We ablated all integrin heterodimers from murine leukocytes, and show here that functional integrins do not contribute to migration in three-dimensional environments. Instead, these cells migrate by the sole force of actin-network expansion, which promotes protrusive flowing of the leading edge. Myosin II-dependent contraction is only required on passage through narrow gaps, where a squeezing contraction of the trailing edge propels the rigid nucleus.


Nature Reviews Immunology | 2008

CCR7 and its ligands: balancing immunity and tolerance

Reinhold Förster; Ana Clara Marques Davalos-Misslitz; Antal Rot

A key feature of the immune system is its ability to induce protective immunity against pathogens while maintaining tolerance towards self and innocuous environmental antigens. Recent evidence suggests that by guiding cells to and within lymphoid organs, CC-chemokine receptor 7 (CCR7) essentially contributes to both immunity and tolerance. This receptor is involved in organizing thymic architecture and function, lymph-node homing of naive and regulatory T cells via high endothelial venules, as well as steady state and inflammation-induced lymph-node-bound migration of dendritic cells via afferent lymphatics. Here, we focus on the cellular and molecular mechanisms that enable CCR7 and its two ligands, CCL19 and CCL21, to balance immunity and tolerance.


European Journal of Immunology | 1999

DISTINCT PATTERNS AND KINETICS OF CHEMOKINE PRODUCTION REGULATE DENDRITIC CELL FUNCTION

Federica Sallusto; Belinda Palermo; Danielle Lenig; Minja Miettinen; Sampsa Matikainen; Ilkka Julkunen; Reinhold Förster; Ralf Burgstahler; Martin Lipp; Antonio Lanzavecchia

Dendritic cells (DC) have been showed to both produce and respond to chemokines. To understand how this may impact on DC function, we analyzed the kinetics of chemokine production and responsiveness during DC maturation. After stimulation with LPS, TNF‐α or CD40 ligand, the inflammatory chemokines MIP‐1α, MIP‐1β and IL‐8 were produced rapidly and at high levels, but only for a few hours, while RANTES and MCP‐1 were produced in a sustained fashion. The constitutive chemokines TARC, MDC and PARC were expressed in immature DC and were up‐regulated following maturation, while ELC was produced only at late time points. Activated macrophages produced a similar spectrum of chemokines, but did not produce TARC and ELC. In maturing DC chemokine production had different impact on chemokine receptor function. While CCR1 and CCR5 were down‐regulated by endogenous or exogenous chemokines, CCR7 levels gradually increased in maturing DC and showed a striking resistance to ligand‐induced down‐regulation, explaining how DC can sustain the response to SLC and ELC throughout the maturation process. The time‐ordered production of inflammatory and constitutive chemokines provides DC with the capacity to self‐regulate their migratory behavior as well as to recruit other cells for the afferent and efferent limb of the immune response.


Journal of Experimental Medicine | 2005

Functional specialization of gut CD103+ dendritic cells in the regulation of tissue-selective T cell homing

Bengt Johansson-Lindbom; Marcus Svensson; Oliver Pabst; Caroline Palmqvist; Gabriel Márquez; Reinhold Förster; William W. Agace

Gut-associated lymphoid tissue (GALT) dendritic cells (DCs) display a unique ability to generate CCR9+ α 4 β 7 + gut-tropic CD8+ effector T cells. We demonstrate efficient induction of CCR9 and α 4 β 7 on CD8+ T cells in mesenteric lymph nodes (MLNs) after oral but not intraperitoneal (i.p.) antigen administration indicating differential targeting of DCs via the oral route. In vitro, lamina propria (LP)–derived DCs were more potent than MLN or Peyers patch DCs in their ability to generate CCR9+ α 4 β 7 + CD8+ T cells. The integrin α chain CD103 (α E) was expressed on almost all LP DCs, a subset of MLN DCs, but on few splenic DCs. CD103+ MLN DCs were reduced in number in CCR7−/− mice and, although CD8+ T cells proliferated in the MLNs of CCR7−/− mice after i.p. but not oral antigen administration, they failed to express CCR9 and had reduced levels of α 4 β 7. Strikingly, although CD103+ and CD103− MLN DCs were equally potent at inducing CD8+ T cell proliferation and IFN-γ production, only CD103+ DCs were capable of generating gut-tropic CD8+ effector T cells in vitro. Collectively, these results demonstrate a unique function for LP-derived CD103+ MLN DCs in the generation of gut-tropic effector T cells.


Journal of Experimental Medicine | 2006

Oral tolerance originates in the intestinal immune system and relies on antigen carriage by dendritic cells

Tim Worbs; Ulrike Bode; Sheng Yan; Matthias W. Hoffmann; Gabriele Hintzen; Günter Bernhardt; Reinhold Förster; Oliver Pabst

Oral tolerance induction is a key feature of intestinal immunity, generating systemic nonresponsiveness to ingested antigens. In this study, we report that orally applied soluble antigens are exclusively recognized in the intestinal immune system, particularly in the mesenteric lymph nodes. Consequently, the initiation of oral tolerance is impeded by mesenteric lymphadenectomy. Small bowel transplantation reveals that mesenteric lymph nodes require afferent lymph to accomplish the recognition of orally applied antigens. Finally, oral tolerance cannot be induced in CCR7-deficient mice that display impaired migration of dendritic cells from the intestine to the mesenteric lymph nodes, suggesting that immunologically relevant antigen is transported in a cell-bound fashion. These results demonstrate that antigen transport via afferent lymphatics into the draining mesenteric lymph nodes is obligatory for oral tolerance induction, inspiring new therapeutic strategies to exploit oral tolerance induction for the prevention and treatment of autoimmune diseases.

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Oliver Pabst

Hannover Medical School

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Elisabeth Kremmer

German Center for Neurodegenerative Diseases

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Martin Lipp

Ludwig Maximilian University of Munich

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Tim Worbs

Hannover Medical School

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Immo Prinz

Hannover Medical School

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Lars Ohl

Hannover Medical School

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Inga Ravens

Hannover Medical School

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