Rejane Flores

Efeito de auxinas sintéticas no enraizamento in vitro da macieira

Apple shoots (Malus domestica, Borkh), cv. Fred Hough derived from in vitro multiplication process were inoculated in MS and MS/2 basal media added by growth substances indol acetic acid (IAA); indol butiric acid (IBA) and naphtalene acetic acid (NAA) at 0, 1, 3 and 5 µM. The media also included: MS vitamins, myo-inositol (100.0 mg/L); sucrose (30.0 g/L); agar (6.0 g/L). The pH was adjusted to 5.8 before autoclaving. The treatments were incubated in a growth room at 25±2oC, 16 hours photoperiod under light intensity of 2,000 lux during 30 days. The treatments were replicated five times. Each replicate was composed by a 250 mL flask containing 40 mL medium with five explants. The medium MS/2 in all the tested concentrations was better than MS. The NAA and IBA, both at 3.0 µM on MS/2 showed similar effects as percentage of rooting and number of roots are concerned. However, NAA treated explants presented a higher callus incidence at the base of shoots along with thicker roots. IAA had a better response when used in higher concentrations but it did not achieve the performance of IBA ou NAA.

Efeito da sacarose e do benomyl na multiplicação in vitro da macieira

The purpose of this work was to study the effect of sucrose and benomyl concentrations on in vitro multiplication of apple (Malus prunifolia Willd, Borkh). The culture media composition was MS basal media plus 1 mg L-1 of BAP. Benomyl was harmful to M. prunifolia at all concentrations. The best sucrose concentrations was 30-45 g L-1, evaluated by shoot and bud number. The sucrose concentration of 45 g L-1 gave the biggest shoot length. The best shoot color was obtained at 15-30 g L-1 of sucrose.

Análises isoenzimáticas em somaclones de morangueiro (fragaria x ananassa Duch.) cv. Vila Nova

Isozymic analyses were used to investigate biochemical variations of somaclones from strawberry cultivar Vila Nova, obtained starting from calli. Analyses were performed using young leaves collected from plants during the in vitro multiplication and afther aclimatation at greenhouse. The enzyme systems used for the analysis of the clones consisted of acid phosphatase and esterase (pH 6.5 and 8.3). During the in vitro culture, two patterns of acid phosphatase were detected among the regenerants, however for the esterase all the genotypes exhibited the same pattern of bands. At greenhouse, were found three patterns of bands different for acid phosphatase. Alterations were not verified in relation to the number and band position for esterase (pH 6.5), while, for esterase (pH 8.3) polymorphism was observed among the clones. Both enzyme systems were influenced by the growth condiction (in vitro and in vivo) and by the developing stage of the plants.