Remi Veneziano
Massachusetts Institute of Technology
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Publication
Featured researches published by Remi Veneziano.
bioRxiv | 2017
Syuan-Ming Guo; Remi Veneziano; Simon Gordonov; Li Li; Demian Park; Anthony Kulesa; Paul C. Blainey; Jeffrey R. Cottrell; Edward S. Boyden; Mark Bathe
Neuronal synapses contain dozens of protein species whose expression levels and localizations are key determinants of synaptic transmission and plasticity. The spectral properties of fluorophores used in conventional microscopy limit the number of measured proteins to four species within a given sample. The ability to perform high-throughput confocal or super-resolution imaging of many proteins simultaneously without limitation in target number imposed by this spectral limit would enable large-scale characterization of synaptic protein networks in situ. Here, we introduce PRISM: Probe-based Imaging for Sequential Multiplexing, a method that sequentially utilizes either high affinity Locked Nucleic Acid (LNA) or low affinity DNA probes to enable diffraction-limited confocal and PAINT-based super-resolution imaging. High-affinity LNA probes offer high-throughput, confocal-based imaging compared with PAINT, which uses low affinity probes to realize localization-based super-resolution imaging. Simultaneous immunostaining of all targets is performed prior to imaging, followed by sequential LNA/DNA probe exchange that requires only minutes under mild wash conditions. We apply PRISM to quantify the co-expression levels and nanometer-scale organization of one dozen cytoskeletal and synaptic proteins within individual neuronal synapses. Our approach is scalable to dozens of target proteins and is compatible with high-content screening platforms commonly used to interrogate phenotypic changes associated with genetic and drug perturbations in a variety of cell types.
Journal of Physical Chemistry Letters | 2017
James L. Banal; Toru Kondo; Remi Veneziano; Mark Bathe; Gabriela S. Schlau-Cohen
Achieving nanoscale spatial and electronic control over the formation of dye aggregates is a major synthetic challenge due to their typically inhomogeneous self-assembly, which limits control over their higher-order organization. To address these challenges, synthetic DNA-templated pseudoisocyanine (PIC) J-aggregates were recently introduced. However, the dependence of the photophysics of the superradiant exciton on the underlying DNA template length and the impact of static disorder on energy transfer through these PIC J-aggregates remain unknown. We examine the delocalization length progression of superradiant PIC excitons by varying the length of poly-A DNA tracts that template PIC J-aggregates. We then investigate the energy-transfer efficiency from PIC J-aggregates with DNA duplex template length, which we found to be limited by static disorder. Utilizing the self-assembled and selective formation of superradiant excitons on DNA provides a platform to determine the function of delocalized excitons in the context of nanoscale energy transport.
bioRxiv | 2017
Remi Veneziano; Tyson R. Shepherd; Sakul Ratanalert; Leila Bellou; Chaoqun Tao; Mark Bathe
Single-stranded DNA (ssDNA) increases the likelihood of homology directed repair with reduced cellular toxic ity, yet ssDNA synthesis strategies are limited by the maximum length attainable, as well control over nucleotide composition. Here, we apply purely enzymatic synthesis to generate ssDNA greater than 15 kb using asymmetric PCR, and illustrate the incorporation of diverse modified nucleotides for therapeutic and imaging applications.
Biophysical Journal | 2015
Syuan-Ming Guo; Remi Veneziano; Russell E. McConnell; Sarit S. Agasti; Simon Gordonov; Tony Kulesa; Frank B. Gertler; Paul C. Blainey; Edward S. Boyden; Peng Yin; Mark Bathe
Protocol exchange | 2018
Remi Veneziano; Syuan-Ming Guo; Li Li; Simon Gordonov; Jeffrey R. Cottrell; Mark Bathe
Nature | 2018
Remi Veneziano; Tyson R. Shepherd; Sakul Ratanalert; Leila Bellou; Chaoqun Tao; Mark Bathe
Biophysical Journal | 2017
Remi Veneziano; Mark Bathe
Biophysical Journal | 2017
Sakul Ratanalert; Remi Veneziano; Mark Bathe
PMC | 2016
Fei Zhang; Hao Yan; Wah Chiu; Remi Veneziano; Sakul Ratanalert; Mark Bathe
Biophysical Journal | 2016
Étienne Boulais; Nicolas P. D. Sawaya; Remi Veneziano; Alessio Andreoni; Su Lin; Neal W. Woodbury; Hao Yan; Alán Aspuru-Guzik; Mark Bathe