Renata Dobosz

MORPHOLOGICAL AND MOLECULAR FEATURES OF PUNCTODERA STONEI BRZESKI, 1998 (NEMATODA: HETERODERIDAE) - SPECIES ASSOCIATED WITH ROOTS OF GRASSES

Abstract. This article provides morphological and molecular characteristics of Punctodera stonei Brzeski, 1998. Comparison of partial sequences of 18S and 28S rDNA genes from P. stonei sampled in Poland and Punctodera sp. from Canada showed their 100% similarity. This is the first report on the occurrence of P. stonei outside of Europe. We provide data on morphology of males and 2nd stage juveniles of this species and an identification key to males of the genus Punctodera Mulvey et Stone, 1976. Moreover, the paper presents evolutionary relationships of P. stonei within the family Heteroderidae.

Comparison of the development ability of the northern root-knot nematode Meloidogyne hapla Chitwood, 1949 on selected species and varieties of Fabaceae Lindl.Porównanie zdolności rozwoju guzaka północnego Meloidogyne hapla Chitwood, 1949 na wybranych gatunkach i odmianach roślin bobowatych Fabaceae Lindl.

Legumes of the genera Vicia, Pisum, Lupinus, Trifolium, Medicago as well as Onobrychis viciifolia, Lotus corniculatus, Ornithopus sativus, Galega orientalis and Lens culinaris were cultivated in pots filled with soil naturally infected with Meloidogyne hapla, under controlled conditions in a glasshouse. Root galls and adult females with egg masses were observed in root systems of all plant species and cultivars included in the experiment. The most numerous occurrence of root galls was recorded on root tissues of Vicia faba, Pisum sativum, Lupinus luteus (cv. Parys) and Trifolium pratense (cv. Krynia) while adult females with egg masses on roots of Trifolium incarnatum, P. sativum (cv. Hubal), Lupinus albus and both cultivars of Medicago sativa. Approximately from 5 to 57 of eggs and juvenile stages were counted in a single mass.