Renata Klebaniuk

Dose-dependent effects of probiotic supplementation on bone characteristics and mineralisation in meat-type female turkeys

To evaluate the influence of the probiotic on bone tissue in female turkeys, bone mineral density and geometrical and mechanical properties of the tibia and femur were determined in a dose-dependent manner (107 colony-forming units (cfu)/g, 108 cfu/g, 109 cfu/g). No effect of the treatments on bone mass and wall thickness of femur was observed, but the administration of the probiotic resulted in the elongation and the reduction of both strengths. The increase in the cross-sectional area of the femur was dose-dependent. Probiotic supplementation at a concentration of 108 cfu/g resulted in a reduction in ultimate strength, but at a concentration of 107 cfu/g, it resulted in the enhancement of the maximum elastic strength of the tibia compared with other groups. The influence of the probiotic administration on tibia geometry was dose-dependent. No effect of the treatments on the relative bone weight and the ratio of mass to length was observed. In general, the influence of the probiotic administration on bone mineral density, bone mineral concentration, bone tissue density, and bone ash, calcium and phosphorus concentrations was dose-dependent. The investigated properties of long bones in female turkeys are affected through probiotic-supplemented diets in a dose-dependent manner. However, on the basis of densitometry, it seems that the administration of the probiotic at a higher concentration of cells is more beneficial for bone development in turkeys.

Gut-bone axis response to dietary replacement of soybean meal with raw low-tannin faba bean seeds in broiler chickens

It seems that faba bean (FB) seeds could be a good protein-energy component in animal feed, but the presence of anti-nutritional substances limits their use as a substitute of soybean meal. The aim of the study was to examine the influence of different concentrations of raw, low-tannin, FB seeds on the gut-bone axis in Ross 308 broilers. One-day old chickens were randomly subjected to one of the 3 dietary treatments: the control group was fed standard diet based on soybean meal and without FB seeds, and two groups were fed 8%/15% and 16%/22% of raw low-tannin FB seeds in the starter and grower, respectively. On the 35th day, hematological and serum biochemical analyses as well histomorphometry of the small intestine and liver tissue and bone mechanical tests were performed. The diet type had no effect on the body weight gain and feed conversion ratio. However, the basal intestinal structures were significantly reduced in birds fed the lower concentration of FB. The enlargement of nerve plexuses was dependent on the concentration used in the diet and, additionally, on the kind of plexus and location in the intestinal tract. The liver was characterized by an increase in non-hepatocytes. There was no influence of the low-tannin FB seeds on most of the analyzed serum parameters in the 35-day-old broiler chickens, except the decreased concentration of total cholesterol and Ca in both experimental groups, triglycerides in group I, and P and uric acid in group II. Furthermore, the increasing concentration of the dietary low-tannin FB did not influence the activities of AspAT (except the group fed the higher amount of FB), ALAT, and LDH. The broiler chickens had no visible leg lesions and no problem in the locomotor function, but the tibiae were lighter mainly in birds fed the higher concentration of FB seeds. Geometric analysis revealed reduction of the cross section area and wall thickness, indicating a decline in the bone midshaft, which influenced the densitometric parameters and the results of mechanical tests. In conclusion, the dietary inclusion of raw low-tannin FB seeds had no negative effects on broiler growth, but disturbed the intestine structure and tibia characteristics. Therefore, all these negative effects necessitate additional examinations before inclusion of raw low-tannin FB seed into poultry diet.

Is Strip Cropping an Effective Way for Maize Biofortyfication

The aim of the study was to assess the impact of strip cropping on the content of nitrogen, phosphorus, potassium, magnesium, and calcium in maize. A field experiment was conducted during 2008–2010 in South Poland. The two cropping methods of maize were studied: sole cropping and strip cropping with common bean and spring barley. Maize was harvested in two different cycle stages: for silage in milky-wax phase and for grain in full maturity phase. Strip cropping significantly increased the accumulation of Mg and Ca by maize biomass and grain. However, the phosphorus content was higher only in maize grain. The row position in the strip influenced the macronutrients content in maize biomass and in grain. The placement adjacent to the bean resulted in higher Ca and Mg content in maize biomass and in grain. The phosphorus content in maize grain was also significantly higher in neighbouring rows with common bean strip. The least amounts of P and N were found in maize from the row adjacent to the barley strip. The results obtained indicated that strip cropping of maize with appropriate plant species, especially pulses, that is, common bean, seems to be an effective way to biofortify maize grain with Ca, especially in regions where it is the staple food. Furthermore, this may mitigate the deficiency of Mg and Ca in maize forage.

Peroxidation and unsaturation indices as potential biomarkers of multifarious zinc and copper micro-supplementation in Helix pomatia L.

The work is a continuation of two previous studies in which biomarker fatty acids (12 of 56 FA pools) were analysed in Helix pomatia L. after heterogeneous micro-supplementation of Zn and Cu (administered in five micro-doses in the form of salts and EDTA and lysine chelates). This time, peroxidation (PI) and unsaturation coefficients (UI) as biomarker were analysed. These indices were calculated based on the FA profile in the foot and hepatopancreas of snails. The correlation of frequently used oxidation status indicators of organisms (catalase - CAT, glutathione peroxidase - GPx, selenium-dependent peroxidase - se-GPx, superoxide dismutase - SOD, glutathione transferase - GST, glutathione reductase - GR, glutathione - GSH, carbonyl protein - CP, thiobarbituric acid reactive substances - TBARS) with the rarely used UI and PI ratios was analysed. It was found that the 12-week micro-exposure to Zn and Cu did not inhibit but rather stimulated antioxidative defence at a sufficient level to increase the values of peroxidation/unsaturation indices in comparison to the control groups. Induction of an opposite process to oxidation of fatty acids was demonstrated. Maximum activities and amounts of antioxidants as well as minima of protein and lipid decomposition were recorded in groups supplemented with 0.75mg/l Zn and 1.0mg/l Cu. The possibility of a direct use of fatty acids as well as peroxidation/unsaturation indices as sensitive and reproducible biomarkers of exposure and oxidative physiological status in snails was confirmed.

The influence of dietary replacement of soybean meal with high-tannin faba beans on gut-bone axis and metabolic response in broiler chickens

Abstract Faba bean (FB) seeds can be a good protein-energy component in animal feed. However, the presence of anti-nutritional substances is a negative feature of FB seeds. The aim of this study was to examine the influence of different levels of unprocessed FB seeds in feed on the gut-bone axis and metabolic profile in broilers. Ninety six, 1-day-old Ross 308 broiler chickens were randomly selected to one of the 3 dietary treatments (32 chickens in each, divided into 8 pens with 4 birds per each pen): the control group fed standard diet with soybean meal and without FB seeds, group I fed 8/15% (starter/grower) of high-tannin FB seeds, and group II fed 16/22% of high-tannin FB seeds. Bone mechanical examination, hematological and serum biochemical analysis as well histomorphometry of small intestine and liver tissue were performed. The intake of high-tannin FB seeds, irrespective of their amount, did not alter the bone geometric, mechanical and densitometric parameters nor influenced basal hematological parameters, however it resulted in: decreased serum concentration of total cholesterol and calcium; a reduced longitudinal myenteron of small intestine; increased mucosa and villus epithelium thickness, villus length, thickness and absorptive surface in duodenum; increased number of active crypts in jejunum; unchanged collagen area, intercellular space, and total cell number in the liver; decreased number of multinuclear hepatocyte cells. Moreover, the livers of birds fed the higher dose of high-tannin FB seeds had lymphocytic infiltrates in portal tracts and sinusoids. Feeding of unprocessed high-tannin FB seeds exerted an influence on the gastrointestinal tract by increased absorptive surface. In conclusion, the dietary inclusion of unprocessed high-tannin FB seeds had no negative effects on broiler growth, tibial bone mechanical properties and intestinal characteristics. Unprocessed high-tannin FB seeds may be used in broiler diets, but their dietary levels should not be higher than those discussed.

Chloramphenicol-Induced Alterations in the Liver and Small Intestine Epithelium in Pigs

Abstract An effect of the exposure to chloramphenicol (CAP) at doses used therapeutically was studied in pigs at the age of slaughter. Pigs were treated with CAP intramuscularly (20 mg/kg b.w. two times every 24 hours). Histomorphometrical and immunohistochemical analyses of small intestine and liver were done. CAP increased the thickness of myenteron and submucosa, and the length of villi; decreased the depth of crypts in the duodenum and jejunum. CAP influenced the Auerbach plexus. A decrease in cell proliferation, an increase in the number of apoptotic cells and T lymphocytes in the CAP-treated pigs were observed. CAP induces hepatotoxicity, neurotoxicity and disturbed intestinal epithelium. It can be concluded that short exposure of pigs to CAP at doses used therapeutically results in disturbed digestion and absorption process in the intestine.

Selected health behaviours of type 2 diabetes patients

Abstract Introduction. Type 2 diabetes is a chronic disease with an epidemic character. Its prevalence is associated with lifestyle, many environmental factors, and genetic determinants. Implementation of diet therapy is the basis for treatment of the disease. Aim. The aim of the study was to assess health behaviours and differences in these behaviours in type 2 diabetes patients taking their age into consideration. Material and methods. The questionnaire survey was carried out among 120 subjects diagnosed with type 2 diabetes. The original questionnaire comprised questions on selected pro-health behaviours, e.g. prophylactic behaviour or self-monitoring in the disease. The statistical analysis was performed on 111 properly completed surveys. The respondents were divided into three age groups: subjects under 60 (n=24), between 60 and 75 (n=64), and over 75 years old (n=23). Results. Regardless of their age, the patients with type 2 diabetes indicated mainly arteriel hypertension as a coexisting complication. The basic element of patients’ self-monitoring was checking the blood pressure and glycaemia. Half of the patients declared body weight control, however, a majority were characterised by excessive body weight and abdominal fat deposits, irrespective of their age. Walking was the most popular form of physical activity chosen by the respondents. Regardless of their age, the type 2 diabetes patients exhibited similar readiness for self-education related to their disease. Conclusions. The surveyed respondents exhibited similar behaviours undertaken to keep healthy. The increase in the regularity of doctor appointments and in the use of pharmacotherapy was closely related to age of the respondents. The advanced age of the respondents had an impact on the regularity of foot inspection. Respondents over 75 years of age adhered to recommendations on the schedule of food and medicine intake, but used diet therapy less frequently.

Pregnant women’s attitudes towards organic food

Abstract Introduction. Proper and healthy nutrition during pregnancy is one of the strongest external determinants of normal development of the foetus. Due to its health-enhancing properties, organic food is its desirable element. Therefore, the analysis of the consumer behaviour associated with organic food among pregnant women seems relevant, in particular given the small number of scientific reports addressing this problem. Aim. The aim of the study was to analyse pregnant women’s behaviour and attitudes towards organic food. The investigations included opinions of the entire group of respondents and was intended to show differences between individual groups of women classified in terms of having children already or not. Material and methods. The survey involved 100 pregnant women living in Lublin and its surroundings, which were assigned into two survey groups: women already having children and those without children. An anonymous questionnaire designed as part of the study was the research tool. The questions were focused on indication of the motivations in the purchase and sources of information about organic food as well as consumer behaviour on the organic food market. Results. A majority of pregnant women assessed their financial situation as satisfactory. The main motive behind buying organic food was their wish to take special care of their health during the pregnancy period and the need to take care of the family. The women were familiar with the concept of organic food. Commercials were the frequent source of information among women expecting the first child and the Internet was the main source of information for those already having children. As specified by the pregnant women, health benefits, naturalness and the absence of chemical contamination are the characteristic features of organic food. Most of the respondents declared an intention to feed their children with organic food to minimise the exposure to chemical contamination. Conclusions. There were differences in the use of sources of information about organic food or the importance of individual determinants of their purchase by the pregnant women. This knowledge can be used on the organic food market to develop marketing solutions, which will be useful in satisfying the needs of this group of consumers more effectively.

Dietary supplementation with glucogenic precusors and fatty acids improves performance and health of periparturient dairy cows

This experiment aimed to make a comparison between the effect of a mix of two energy preparations (a glucogenic preparation (G) and a free fatty acid preparation (F)), administered in the diet during the transition period over different periods of time (5 or 8 weeks), on the animal condition, feed intake, yield and composition of milk, and blood and urine parameters. The experimental additive was a mixture (1 : 1) of two energy preparations, namely, a loose mixture of propylene glycol and sodium propionate and a free fatty acid preparation in the form of rumen-undegradable pellets. The study included 50 Polish Black-and-White Holstein–Friesian dairy cows, allocated into five treatment groups, including one control (C) and four experimental ones (G1F1, G2F2, G2F1 and G1F2). The preparations were mixed at a 1 : 1 weight ratio (500 g per head, comprising 250G and 250F) and they were administered to all experimental groups of cows daily as a component of the experimental concentrate for 5 weeks (from the 2nd week before the expected calving and until the 3rd week of lactation). From the 4th until the 6th week of lactation, the supplementation in the experimental groups changed and animals received either no additive (G1F1), or were continuously supplemented with the G–F mix (G2F2), or received only G (G2F1) or only F (G1F2). The preparations used in the study had a long-term influence on the increase in the milk yield of cows, on the improvement of their health condition, and on the reduction of weight loss. The study results confirmed that prolongation of the supplementation until the 6th week of lactation may cause favourable changes in animal performance and milk quality. Due to different modes of action, the simultaneous supplementation of G and F reduced the negative energy balance in the cow and improved milk production and composition. The best results were obtained after administration of the mixture (1 : 1) of the G and F at the dose of 500 g/cow.day over the 8-week periparturient period.