Renata Pimentel Bandeira de Melo
Universidade Federal Rural de Pernambuco
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Acta Tropica | 2015
José Givanildo da Silva; Bruno H.L.S. Alves; Renata Pimentel Bandeira de Melo; Pomy de Cássia Peixoto Kim; Orestes Luiz de Souza Neto; Mauro José G. Bezerra; Silvio Gomes de Sá; Rinaldo Aparecido Mota
The aim of the present study was to detect Toxoplasma gondii DNA in raw milk samples of goats and sheep of local breeds from the semi-arid region of the states of Pernambuco and Paraíba, Northeastern Brazil. Serum and milk samples were collected from 243 animals (186 goats and 57 sheep). The Indirect fluorescent antibody test (IFAT) was used to search for anti-T. gondii IgG antibodies with a cutoff of 64. Subsequently, the raw milk samples were subjected to DNA extraction and PCR to detect DNA of T. gondii. The IFAT results showed a 6.58% (16/243) positivity when all the samples were considered and a positivity of 15.78% (9/57) and 3.76% (7/186) for goats and sheep samples, respectively. The PCR assay detected T. gondii DNA in 2.06% (5/243) of all the samples tested. All the PCR positive samples were from goats. This result shows the importance of adopting measures of flocks sanitary management and avoiding the consumption of raw milk may constitute a potential risk to the health of milk consumers in this region.
Pesquisa Veterinaria Brasileira | 2013
Mauro José Gonçalves Bezerra; Jefferson Ayrton Leite de Oliveira Cruz; Eugênio de Souza Kung; Renata Pimentel Bandeira de Melo; Ana Lisa do Vale Gomes; Érica Paes Barreto Xavier de Moraes; José Wilton Pinheiro Junior; Rinaldo Aparecido Mota
The aim of the study was to detect genomic DNA of Toxoplasma gondii in testicle and epididymis samples from rams sold in abattoirs in the state of Pernambuco, Northeast Brazil. Fifty (50) blood serum samples were collected, as well as 50 testicle and epididymis samples. Indirect Immunofluorescence (IIF) was used during screening of the rams. The Polymerase Chain Reaction (PCR) was used with animals that were positive in serology. Our results confirmed that 24% (12/50) of the rams were positive in IIF. Genomic DNA was detected in the epididymis at 8.3% (1/12) of the animals. The molecular identity of the amplified products was confirmed through sequencing. This paper reports the first occurrence of T. gondii DNA in the reproductive organs of naturally infected rams in Brazil.
Acta Tropica | 2017
Jonatas Campos de Almeida; Renata Pimentel Bandeira de Melo; Camila de Morais Pedrosa; Marcelo da Silva Santos; Luiz Daniel de Barros; João Luis Garcia; Wagnner José Nascimento Porto; Rinaldo Aparecido Mota
Wild animals may play an important role in the transmission and maintenance of Toxoplasma gondii in the environment. The purpose of the present study was to isolate and genotype T. gondii from a free-ranging crab-eating fox (Cerdocyon thous-Linnaeus, 1766). A crab-eating fox in critical health condition was attended in a veterinary hospital in Recife, Pernambuco State, Brazil. The animal died despite emergency treatment. The brain was collected aseptically and destined for mouse bioassay. One isolate of T. gondii was obtained, and Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP) was used to assess genetic variability at 11 markers (SAG1, SAG2, altSAG2, SAG3, BTUB, GRA6, c228, c292, L358, PK1 and APICO). A murine model was used to assess the virulence of the isolate. Using the PCR-RFLP, genotype ToxoDB #13 was identified, which is considered an atypical strain. The isolate was classified as avirulent in the murine model. This is the first study to report T. gondii infection in the crab-eating fox.
Veterinary Parasitology | 2016
Renata Pimentel Bandeira de Melo; Jonatas Campos de Almeida; D.C.V. Lima; Camila de Morais Pedrosa; F.J.R. Magalhães; A.M. Alcântara; Luiz Daniel de Barros; Rafael Felipe da Costa Vieira; João Luis Garcia; Rinaldo Aparecido Mota
Toxoplasma gondii isolates from Brazil have a different phenotypic and genotypic pattern, with predominance of virulent isolates and recombinant genotypes, compared to the North Hemisphere. Considering that a new T. gondii genotype, non-pathogenic to mice, was previously identified from free-range chickens from the Fernando de Noronha Island, Brazil, this study aimed to identify genotypes of this parasite in tissue samples of feral cats (Felis catus) from this Brazilian Island. Anti-T. gondii IgG antibodies were detected in 18/31 (58%) feral cats. Two non-virulent T. gondii isolates were obtained by mouse bioassay. Genotyping was performed by PCR-RFLP using 10 genetic markers (SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, PK1, L358 and Apico) and an atypical strain of T. gondii (ToxoDB #146) was identified. This is the first report of this genotype in feral cats.
Tropical Animal Health and Production | 2016
Marilene de Farias Brito; Alessandra dos Santos Belo-Reis; José Diomedes Barbosa; D. G. Ubiali; Ana Paula de Castro Pires; Elizabeth Sampaio de Medeiros; Renata Pimentel Bandeira de Melo; Pedro Paulo Feitosa de Albuquerque; Elise Myuki Yamasaki; Rinaldo Aparecido Mota
Several farms in the Northeast of Brazil were investigated for Mycobacterium avium subsp. paratuberculosis infection in order to identify the occurrence of paratuberculosis in buffaloes. Samples were obtained from 17 farms, two slaughter houses, and a quarantine area in the Northeast. About 15,000 buffaloes of the Murrah, Mediterranean, and Jafarabadi breed as well as their crossbreeds were evaluated for meat, dairy, and mixed farms with semi-intensive or extensive breeding practices. For diagnostic purposes, postmortem and histopathological examination, including Ziehl-Neelsen test of fecal smears and scraped intestinal mucosa were performed. PCR was applied for fecal samples, mesenteric lymph nodes, and intestines. Six Johne’s disease-positive farms, which together with those previously identified, indicate that the disease is spread through the Brazilian Northeast, similar to what occurs in cattle herds in other regions of the country. The increase in prevalence of paratuberculosis is a consequence of introduction of animals from other regions without adequate veterinary assistance and due to the little official attention paid to this initially silent and chronic disease.
Parasitology Research | 2015
Erika Fernanda Torres Samico-Fernandes; Renata Pimentel Bandeira de Melo; Pomy de Cássia Peixoto Kim; Jonatas Campos de Almeida; Luiz Daniel de Barros; João Luis Garcia; Jean Carlos Ramos Silva; Rinaldo Aparecido Mota
The aim of the present study was to isolate and genotype Toxoplasma gondii from pigs slaughtered for human consumption in northeastern Brazil. Indirect immunofluorescence antibody test (IFAT) was used to screen positive pigs. Tissues samples of animals with antibody titers ≥64 were submitted to bioassay in mice. One isolate of T. gondii was obtained, and the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique, using 11 markers (SAG1, SAG2, altSAG2, SAG3, BTUB, GRA6, c228, c292, L358, PK1, and APICO), was applied to evaluate the genetic variability. DNA from reference strains was used as a positive control. By means of genetic analysis, genotype ToxoDB #65 was identified, which is considered an atypical strain. This is the first record of genotype #65 in pigs. Thus, further studies in this region are necessary to determine the genetic variability of T. gondii in pigs and possible impact on public health.
Acta Parasitologica | 2016
Débora Costa Viegas de Lima; André de Souza Santos; Luana Thamires Rapôso da Silva; Renata Pimentel Bandeira de Melo; José Givanildo da Silva; José Wilton Pinheiro Junior; Rinaldo Aparecido Mota
The present study aimed to conduct a serosurvey of toxoplasmosis in domestic rabbits of northeastern Brazil. Blood samples and tissue fragments (brain, heart and diaphragm) were collected from 150 and 54 rabbits from the state of Pernambuco, Brazil, respectively. The serum samples were subjected to serological analysis (Modified Agglutination Test - MAT) and the tissue samples were assessed by PCR and histopathological analysis. Data collected through questionnaires were subjected to analysis of risk factors. According to the MAT and the PCR results, 6.7% (10/150; CI 3.2%–11.9%) of the rabbits were positive for anti- T. gondii antibodies and 9.25% (5/54) of the tissue fragments were positive for T. gondii DNA, respectively. Lesions associated with T. gondii infection, mainly characterized by granuloma, mononuclear cell infiltrates, degeneration areas and necrosis in brain and heart, were detected in the histopathological analysis. The risk factors associated with T. gondii infection identified in the present study were homade food (odds ratio = 39.00) and contact between cats and rabbits (odds ratio = 52.00). This is the first report of toxoplasmosis in rabbits of northeastern Brazil. The management problems identified in the present study must be corrected to reduce the frequency of positive animals in herds of rabbits.
Tropical Animal Health and Production | 2018
Pedro Paulo Feitosa de Albuquerque; Renata Pimentel Bandeira de Melo; Marilene de Farias Brito; Fernanda Bovino; Mariana Assunção de Souza; Anna Monteiro Correia Lima; Emerson Antônio Araújo de Oliveira; Helder de Moraes Pereira; Rinaldo Aparecido Mota
Paratuberculosis is an incurable disease in ruminants with great worldwide economic impact, caused by Mycobacterium avium subsp. paratuberculosis (MAP). The objective of this study was to carry out a study of the molecular epidemiology of the MAP using the restriction enzyme analysis (REA) technique of IS1311 MAP region in biological samples of feces, intestinal tissue, and mesenteric lymph nodes of cattle and buffaloes from six Brazilian states. In total, 109 samples of feces and tissues of cattle and buffaloes were collected from animal paratuberculosis suspected. Twenty-five samples were positive in the detection of the DNA of the IS900 region of MAP and it was possible to type 18 strains in the analysis of the region IS1311, being 100% of them identified as belonging to subtype Bison MAP strain. This is the first epidemiological molecular study of MAP in Brazil. The results indicate that paratuberculosis is widespread in cattle and in buffaloes in several regions of Brazil, and the subtype Bison MAP strain was the only one identified in the samples analyzed in this study, demonstrating the similarity between the strains from different states tested. These results provide the necessary support for the implementation of paratuberculosis control strategies in cattle and buffaloes in Brazil.
Brazilian Archives of Biology and Technology | 2018
Jonatas Campos de Almeida; Carlos Adriano de Santana Leal; Renata Pimentel Bandeira de Melo; Pedro Paulo Feitosa de Albuquerque; Camila de Morais Pedrosa; Fabiana Correa Zermiani; Roberto Citelli de Farias; Rinaldo Aparecido Mota
The aim of the present study was to describe the clinical manifestation, treatment and outcome of a case of coinfection by Sarcoptes scabiei and Microsporum gypseum in Cerdocyon thous (crab-eating fox) from Northeastern Brazil.
Acta Parasitologica | 2018
Jonatas Campos de Almeida; Renata Pimentel Bandeira de Melo; Pomy de Cássia Peixoto Kim; Neurisvan Ramos Guerra; Leucio Câmara Alves; Diego Figueiredo da Costa; Clebert José Alves; Wagnner José Nascimento Porto; Rinaldo Aparecido Mota
The aim of this study was to detect DNA and antibodies anti-Leishmania spp., Neospora caninum and Toxoplasma gondii in captive and free-range crab-eating fox (Cerdocyon thous) from northeastern Brazil. Twenty-five crab-eating foxes from different states of northeastern Brazil were sampled by this study. Blood samples were collected by cephalic or jugular vein punctures. The whole blood was submitted to PCR, and the sera samples to the serological analysis as follows: MAT for T. gondii, NAT for N. caninum, and ELISA for L. chagasi. The frequence of antibodies anti-T. gondii was 50% and 29.41% for free-range and captive wild canids, respectively. The frequence of antibodies anti-N. caninum observed by this study was 62.50% and 23.52% for free-range and captive wild canids, respectively. The frequence of antibodies anti-L. chagasi was 4.0% for captive wild canids. Co-infections cases were identified as follows: one captive wild canid seropositive for T. gondii and L. chagasi and two free-range animals seropositive for T. gondii and N. caninum. All PCR assays performed were negative for the pathogens analyzed. This study describes the presence of antibodies anti-T. gondii, N. caninum e L. chagasi in wild canids from northeastern Brazil and highlights the necessity of further studies on infectious diseases in free-range and captive wild canids.
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Pedro Paulo Feitosa de Albuquerque
Universidade Federal Rural de Pernambuco
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