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Dive into the research topics where Rene Beutner is active.

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Featured researches published by Rene Beutner.


Journal of the Royal Society Interface | 2010

Biological nano-functionalization of titanium-based biomaterial surfaces: a flexible toolbox

Rene Beutner; Jan Michael; Bernd Schwenzer; Dieter Scharnweber

Surface functionalization with bioactive molecules (BAMs) on a nanometre scale is a main field in current biomaterial research. The immobilization of a vast number of substances and molecules, ranging from inorganic calcium phosphate phases up to peptides and proteins, has been investigated throughout recent decades. However, in vitro and in vivo results are heterogeneous. This may be at least partially attributed to the limits of the applied immobilization methods. Therefore, this paper highlights, in the first part, advantages and limits of the currently applied methods for the biological nano-functionalization of titanium-based biomaterial surfaces. The second part describes a new immobilization system recently developed in our groups. It uses the nanomechanical fixation of at least partially single-stranded nucleic acids (NAs) into an anodic titanium oxide layer as an immobilization principle and their hybridization ability for the functionalization of the surface with BAMs conjugated to the respective complementary NA strands.


Journal of Materials Science: Materials in Medicine | 2002

Electrochemical behavior of titanium-based materials – are there relations to biocompatibility?

Dieter Scharnweber; Rene Beutner; Sophie Rößler; Hartmut Worch

For biomedical applications the physico-chemical properties of oxide layers, always present in titanium-based materials, are of special interest because the biological system is in direct contact only with these oxides. Using electrochemical impedance spectroscopy and galvanostatic polarization it is shown that the different compositions of c.p.-titanium, Ti6Al4V, and Ti6Al7Nb result in different physico-chemical properties of air formed passive layers and anodic oxide layers. This may have a direct impact on the biocompatibility of these materials. Results of impedance spectroscopy distinctly differ in the flatband potentials as well as in the donor densities of air-formed passive layers with Ti6Al7Nb showing an approximately 50% smaller donor density than the other materials. Anodic galvanostatic polarization results in voltage–charge density curves with distinct differences in the Faraday efficiency ∈ of the oxide formation between Ti6Al7Nb and c.p.-titanium/Ti6Al4V, especially for low current densities. These effects correlate strongly with the donor densities in the air formed passive films of the examined materials. SEM-images of anodic oxide layers show a blister containing surface morphology of the outer part of the oxide layers for all materials. This morphology is probably caused by oxygen evolution, a process which relies on the transfer of electrons through the growing anodic oxide layers and strongly depends on the donor density in the air formed passive layers. Again, the much more pronounced morphology on c.p. titanium/Ti6Al4V agrees with the different donor densities in the air formed passive layers on the materials. These findings correlate with the good biocompatibility of Ti6Al7Nb and suggest that conduction mechanisms, in air formed passive layers and anodic oxide layers, contribute to processes that determine the biocompatibility of these materials.


Biomaterials | 2009

Immobilization of oligonucleotides on titanium based materials by partial incorporation in anodic oxide layers.

Rene Beutner; Jan Michael; Anne Förster; Bernd Schwenzer; Dieter Scharnweber

This paper describes the immobilization of bioactive molecules on titanium based surfaces through a combination of nano-mechanical fixation of nucleic acid anchor strands (ASs) by partial and regioselective incorporation within an anodic oxide layer and their hybridization with complementary strands (CSs) intended to be conjugated to bioactive molecules. We focus on the interaction between the substrate surface and the anchor strands, the integrity of ASs and their hybridization ability. The observed dependence of adsorption on pH suggests that initial interaction of terminally phosphorylated ASs with the substrate surface is mediated by electrostatic interaction. Using ASs labelled with (32)P at different termini, it could be shown that strand breaks occur, which are attributed (i) to the formation of reactive oxygen species during anodic polarization, (ii) the photocatalytic activity of the titanium oxide and (iii) drying effects. Damage to AS could be considerably reduced if the electrolyte contained 5 mol l(-1) ethanol, light was excluded during the experimental procedure, and the number of drying and rewetting steps was minimized. A total surface density of AS of 4.5 pmol cm(-2) was reached and could be hybridized to CS with an efficiency of up to 100%. A non-complementary strand (NS) bound with less than 0.5% of the amount of CS under similar conditions. Therefore, non-specific binding of CS is considered as negligible.


Materials Science and Engineering: C | 2016

Electrochemically assisted deposition of hydroxyapatite on Ti6Al4V substrates covered by CVD diamond films - Coating characterization and first cell biological results.

Paulina Strąkowska; Rene Beutner; Marcin Gnyba; Andrzej Zieliński; Dieter Scharnweber

Although titanium and its alloys are widely used as implant material for orthopedic and dental applications they show only limited corrosion stability and osseointegration in different cases. The aim of the presented research was to develop and characterize a novel surface modification system from a thin diamond base layer and a hydroxyapatite (HAp) top coating deposited on the alloy Ti6Al4V widely used for implants in contact with bone. This coating system is expected to improve both the long-term corrosion behavior and the biocompatibility and bioactivity of respective surfaces. The diamond base films were obtained by Microwave Plasma Assisted Chemical Vapor Deposition (MW-PACVD); the HAp coatings were formed in aqueous solutions by electrochemically assisted deposition (ECAD) at varying polarization parameters. Scanning electron microscopy (SEM), Raman microscopy, and electrical conductivity measurements were applied to characterize the generated surface states; the calcium phosphate coatings were additionally chemically analyzed for their composition. The biological properties of the coating system were assessed using hMSC cells analyzing for cell adhesion, proliferation, and osteogenic differentiation. Varying MW-PACVD process conditions resulted in composite coatings containing microcrystalline diamond (MCD/Ti-C), nanocrystalline diamond (NCD), and boron-doped nanocrystalline diamond (B-NCD) with the NCD coatings being dense and homogeneous and the B-NCD coatings showing increased electrical conductivity. The ECAD process resulted in calcium phosphate coatings from stoichiometric and non-stoichiometric HAp. The deposition of HAp on the B-NCD films run at lower cathodic potentials and resulted both in the highest coating mass and the most homogenous appearance. Initial cell biological investigations showed an improved cell adhesion in the order B-NCD>HAp/B-NCD>uncoated substrate. Cell proliferation was improved for both investigated coatings whereas ALP expression was highest for the uncoated substrate.


Beilstein Journal of Organic Chemistry | 2014

Detonation nanodiamonds biofunctionalization and immobilization to titanium alloy surfaces as first steps towards medical application.

Juliana P L Gonçalves; Afnan Q Shaikh; Manuela Reitzig; Daria A. Kovalenko; Jan Michael; Rene Beutner; Gianaurelio Cuniberti; Dieter Scharnweber; Jörg Opitz

Summary Due to their outstanding properties nanodiamonds are a promising nanoscale material in various applications such as microelectronics, polishing, optical monitoring, medicine and biotechnology. Beyond the typical diamond characteristics like extreme hardness or high thermal conductivity, they have additional benefits as intrinsic fluorescence due to lattice defects without photobleaching, obtained during the high pressure high temperature process. Further the carbon surface and its various functional groups in consequence of the synthesis, facilitate additional chemical and biological modification. In this work we present our recent results on chemical modification of the nanodiamond surface with phosphate groups and their electrochemically assisted immobilization on titanium-based materials to increase adhesion at biomaterial surfaces. The starting material is detonation nanodiamond, which exhibits a heterogeneous surface due to the functional groups resulting from the nitrogen-rich explosives and the subsequent purification steps after detonation synthesis. Nanodiamond surfaces are chemically homogenized before proceeding with further functionalization. Suspensions of resulting surface-modified nanodiamonds are applied to the titanium alloy surfaces and the nanodiamonds subsequently fixed by electrochemical immobilization. Titanium and its alloys have been widely used in bone and dental implants for being a metal that is biocompatible with body tissues and able to bind with adjacent bone during healing. In order to improve titanium material properties towards biomedical applications the authors aim to increase adhesion to bone material by incorporating nanodiamonds into the implant surface, namely the anodically grown titanium dioxide layer. Differently functionalized nanodiamonds are characterized by infrared spectroscopy and the modified titanium alloys surfaces by scanning and transmission electron microscopy. The process described shows an adsorption and immobilization of modified nanodiamonds on titanium; where aminosilanized nanodiamonds coupled with O-phosphorylethanolamine show a homogeneous interaction with the titanium substrate.


Acta of Bioengineering and Biomechanics | 2017

Deposition of phosphate coatings on titanium within scaffold structure

Bartłomiej Trybuś; Andrzej Zieliński; Rene Beutner; Tomasz Seramak; Dieter Scharnweber

PURPOSE Existing knowledge about the appearance, thickness, and chemical composition of phosphate coatings on titanium inside porous structures is insufficient. Such knowledge is important for the design and fabrication of porous implants. METHODS Metallic scaffolds were fabricated by selective laser melting of 316L stainless steel powder. Phosphate coatings were deposited on Ti sensors placed either outside the scaffolds or in the holes in the scaffolds. The electrochemically-assisted cathodic deposition of phosphate coatings was performed under galvanostatic conditions in an electrolyte containing the calcium and phosphate ions. The phosphate deposits were microscopically investigated; this included the performance of mass weight measurements and chemical analyses of the content of Ca2+ and  24 PO ions after the dissolution of deposits. RESULTS The thicknesses of the calcium phosphate coatings were about 140 and 200 nm for isolated titanium sensors and 170 and 300 nm for titanium sensors placed inside pores. Deposition of calcium phosphate occurred inside the pores up to 150 mm below the scaffold surface. The deposits were rich in Ca, with a Ca/P ratio ranging from 2 to 2.5. CONCLUSIONS Calcium phosphate coatings can be successfully deposited on a Ti surface inside a model scaffold. An increase in cathodic current results in an increase in coating thickness. Any decrease in the cathodic current inside the porous structure is slight. The calcium phosphate inside the pores has a much higher Ca/P ratio than that of stoichiometric HAp, likely due to a gradual increase in Ca fraction with distance from the surface.


Surface Engineering | 2017

Properties of composite oxide layers on the Ti13Nb13Zr alloy

A. Ossowska; Rene Beutner; Dieter Scharnweber; Andrzej Zieliński

ABSTRACT The development of composite oxide layers on the Ti13Nb13Zr alloy, their structure and properties, were demonstrated. Two subsequent methods were applied to prepare the composite layers. During the first stage, gas oxidation produced a dense compact oxide layer, and subsequent oxide nanotubes were formed applied an electrochemical oxidation. The scanning electron microscopy, energy-dispersive X-ray spectroscopy, electrochemical impedance spectroscopy, and Raman spectroscopy were used to examine the appearance, thickness, chemical and phase composition of the oxide layers. The results obtained revealed that the composite layers composed of two zones. The electrochemical formation of the outer nanotubular oxide zone occurred if the thickness of the first inner oxide zone was small enough because of resistance increasing with the inner zone thickness followed by a decrease in electrochemical reaction rate. The decreasing corrosion resistance of the Ti13Nb13Zr alloy was observed and explained by a model electric circuit.


Biomaterials | 2005

Osteoconductive modifications of Ti-implants in a goat defect model: characterization of bone growth with SR μCT and histology

Ricardo Bernhardt; Juliette van den Dolder; Sussane Bierbaum; Rene Beutner; Dieter Scharnweber; John A. Jansen; Felix Beckmann; Hartmut Worch


Bioconjugate Chemistry | 2009

Oligonucleotide−RGD Peptide Conjugates for Surface Modification of Titanium Implants and Improvement of Osteoblast Adhesion

Jan Michael; Lena Schönzart; Ina Israel; Rene Beutner; Dieter Scharnweber; Hartmut Worch; Ute Hempel; Bernd Schwenzer


Journal of Biomedical Materials Research Part A | 2003

Modification of Ti6Al4V surfaces using collagen I, III, and fibronectin. I. Biochemical and morphological characteristics of the adsorbed matrix

Susanne Bierbaum; Rene Beutner; Thomas Hanke; Dieter Scharnweber; Ute Hempel; Hartmut Worch

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Hartmut Worch

Dresden University of Technology

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Jan Michael

Dresden University of Technology

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Bernd Schwenzer

Dresden University of Technology

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Thomas Hanke

Dresden University of Technology

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Sophie Roessler

Dresden University of Technology

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