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Dive into the research topics where Ricardo E. Oñate-Sánchez is active.

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Featured researches published by Ricardo E. Oñate-Sánchez.


Journal of Craniofacial Surgery | 2013

Cell therapy in bisphosphonate-related osteonecrosis of the jaw.

Mar Gonzálvez-García; Francisco Javier Rodríguez-Lozano; Victor Villanueva; Daniel Segarra-Fenoll; Maria Angeles Rodríguez-González; Ricardo E. Oñate-Sánchez; Miguel Blanquer; José María Moraleda

ObjectiveBisphosphonate-related osteonecrosis of the jaw (BRONJ) is a clinical condition found in patients who have received intravenous or oral bisphosphonate therapy for various diseases related to bone. This report describes a novel treatment of BRONJ using autologous bone marrow stem cells, platelet-rich plasma, beta tricalcium phosphate, and demineralized bone matrix. Study DesignWe report a 71-year-old woman with history of multiple myeloma treated with intravenous zoledronic acid during 4 years. After a tooth extraction, the patient presented with a painful BRONJ lesion with no healing wound and cortical bone exposure. The patient was surgically managed with a standardized protocol of autologous stem cell therapy combining bone marrow harvest, cell concentration procedures, and intraoral surgery. ResultsCT scan performed 6 months later showed improvement of bone and concentric ossification. Cellular therapy might be considered a new strategy to heal BRONJ lesions.


Journal of Endodontics | 2017

Cytotoxicity of GuttaFlow Bioseal, GuttaFlow2, MTA Fillapex, and AH Plus on Human Periodontal Ligament Stem Cells

Mar Collado-González; Christopher J. Tomás-Catalá; Ricardo E. Oñate-Sánchez; José M. Moraleda; Francisco Javier Rodríguez-Lozano

Introduction: The aim of the present study was to evaluate the in vitro cytotoxicity of endodontic sealers (GuttaFlow Bioseal, GuttaFlow2, and MTA Fillapex) on human periodontal ligament stem cells (hPDLSCs). As a reference, AH Plus was compared with the more recent endodontic sealers regarding cell viability and cell attachment. Methods: Biological testing was carried out in vitro on hPDLSCs. Cell viability assay was performed by using eluates from each endodontic sealer. To assess cell morphology and attachment to the different sealers, the hPDLSCs were directly seeded onto the material surfaces and analyzed by scanning electron microscopy. Chemical composition of the sealers was determined by energy‐dispersive x‐ray, and eluates were analyzed by inductively coupled plasma mass spectrometry. Statistical differences were assessed by analysis of variance and Tukey test (P < .05). Results: Cell viability was evident after 24 hours in the presence of GuttaFlow Bioseal and GuttaFlow 2 but not in the case of AH Plus or MTA Fillapex. At 168 hours, GuttaFlow Bioseal and GuttaFlow 2 exhibited high and moderate cell viability, respectively, whereas AH Plus and MTA Fillapex revealed low rates of cell cell viability (P < .001). Finally, scanning electron microscopy studies revealed a high degree of proliferation, cell spreading, and attachment, especially when using GuttaFlow Bioseal disks. Conclusions: GuttaFlow Bioseal and GuttaFlow2 showed lower cytotoxicity than MTA Fillapex and AH plus. Further in vitro and in vivo investigations are required to confirm the suitability of GuttaFlow Bioseal for clinical application. HIGHLIGHTSThe sealerss extracts caused dose‐ and time‐dependent effects on hPDLSCs.GuttaFlow Bioseal exhibited better cytocompatibility than the other sealers.Further in vitro and in vivo investigations of GuttaFlow Bioseal are required.


Journal of Endodontics | 2018

Biocompatibility of New Pulp-capping Materials NeoMTA Plus, MTA Repair HP, and Biodentine on Human Dental Pulp Stem Cells

Christopher J. Tomás-Catalá; Mar Collado-González; David García-Bernal; Ricardo E. Oñate-Sánchez; Leopoldo Forner; Carmen Llena; A. Lozano; José M. Moraleda; Francisco Javier Rodríguez-Lozano

Introduction: The aim of the present study was to evaluate the in vitro cytotoxicity of MTA Repair HP, NeoMTA Plus, and Biodentine, new bioactive materials used for dental pulp capping, on human dental pulp stem cells (hDPSCs). Methods: Biological testing was carried out in vitro on hDPSCs. Cell viability and cell migration assays were performed using eluates of each capping material. To evaluate cell morphology and cell attachment to the different materials, hDPSCs were directly seeded onto the material surfaces and analyzed by scanning electron microscopy. The chemical composition of the pulp‐capping materials was determined by energy‐dispersive X‐ray and eluates were analyzed by inductively coupled plasma‐mass spectrometry. Statistical differences were assessed by analysis of variance and Tukey test (P < .05). Results: Cell viability was moderate after 24 and 48 hours in the presence of MTA Repair HP and NeoMTA Plus, whereas at 48 and 72 hours, Biodentine showed higher rates of cell viability than MTA Repair HP and NeoMTA Plus (P < .001). A cell migration assay revealed adequate cell migration rates for MTA Repair HP and NeoMTA Plus, both similar to the control group rates, meanwhile the highest cell migration rate was observed in the presence of Biodentine (P < .001). Scanning electron microscope studies showed a high degree of cell proliferation and adhesion on Biodentine disks but moderate rates on MTA Repair HP and NeoMTA Plus disks. Energy‐dispersive X‐ray pointed to similar weight percentages of C, O, and Ca in all 3 materials, whereas other elements such as Al, Si, and S were also found. Conclusions: The new pulp‐capping materials MTA Repair HP, NeoMTA Plus, and Biodentine showed a suitable degree of cytocompatibility with hDPSCs, and good cell migration rates, although Biodentine showed higher rates of proliferation time‐dependent. HighlightsResults of this work suggest that hDPSCs show suitable biological response in terms of cell viability, cell proliferation, and cell migration when in contact with extracts from NeoMTA Plus, MTA Repair HP, and Biodentine.NeoMTA Plus and MTA Repair HP exhibited similar cytocompatibility and cell attachment with hDPSCs, whereas Biodentine promotes higher proliferation rates, cell migration, and cell attachment.Further in vitro and in vivo investigations are required to contrast these results and to prove suitable clinical applications of NeoMTA Plus and MTA Repair HP.


Translational Research | 2015

Potential of graphene for tissue engineering applications.

Francisco Javier Rodríguez-Lozano; David García-Bernal; Salvador Aznar-Cervantes; Ricardo E. Oñate-Sánchez; José M. Moraleda

Q2 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 To Editor, We read with great interest the article published by Empson et al. The authors suggested the use of nanocarbon particles as graphene to replace damaged connective tissues. In fact, nanocarbons including carbon nanotubes, carbon nanohorns, and graphenes have also shown an enhancement of matrix mechanical properties; their small size and functional capabilities make them attractive therapeutic options as nanofillers for many regenerative medicine applications. In our recent study, we evaluated the effects of the novel biomaterials graphene oxide (GO) and silk fibroin composites on human periodontal ligament mesenchymal stem cells (PDLSCs) phenotype, adhesion, proliferation rate, and viability. Biocompatibility of scaffolds is a prerequisite for generating cell-biomaterial constructs and for their successful clinical application. GO and fibroin-based biomaterials have been previously studied for several tissue engineering based-therapies, but they have never been tested in conjunction with PDLSCs. Morphologic studies by staining of actin cytoskeleton of PDLSCs cultured for different times on GO and fibroin-coated surfaces showed that PDLSCs cultured on fibroin films displayed lower F-actin polymerization, lower cell spreading, and delayed initial adhesion to the biomaterial. By contrast, GO or GO plus fibroin-coated surfaces significantly improved F-actin polymerization, cell spreading, and initial adhesion when compared with fibroin alone. Furthermore, the proliferation rate and phenotype of PDLSCs on GO and fibroin-based biomaterials by MTT colorimetric assays and flow cytometry, respectively, were studied.


Materials | 2018

Human Dental Pulp Stem Cells Exhibit Different Biological Behaviours in Response to Commercial Bleaching Products

Carmen Llena; Mar Collado-González; Christopher J. Tomás-Catalá; David García-Bernal; Ricardo E. Oñate-Sánchez; Francisco Javier Rodríguez-Lozano; Leopoldo Forner

The purpose of this study was to evaluate the diffusion capacity and the biological effects of different bleaching products on human dental pulp stem cells (hDPSCs). The bleaching gel was applied for 90, 30 or 15 min to enamel/dentine discs that adapted in an artificial chamber. The diffusion of hydrogen peroxide (HP) was analysed by fluorometry and the diffusion products were applied to hDPSCs. Cell viability, cell migration and cell morphology assays were performed using the eluates of diffusion products. Finally, cell apoptosis and the expression of mesenchymal stem cell markers were analysed by flow cytometry. Statistical analysis was performed using analysis of variance and Kruskal–Wallis or Mann–Whitney tests (α < 0.05). Significant reductions of approximately 95% in cell viability were observed for the 3 × 15 min groups (p < 0.001), while 1 × 30 min of PerfectBleach and 1 × 90 min of PolaNight resulted in reductions of 50% and 60% in cell viability, respectively (p < 0.001). Similar results were obtained in the migration assay. Moreover, the 3 × 15 min group was associated with cell morphology alterations and reductions of >70% in cell live. Finally, hDPSCs maintained their mesenchymal phenotype in all conditions. Similar concentrations of carbamide peroxide (CP) and HP in different commercial products exhibited different biological effects on hDPSCs.


Dental Materials | 2018

Thermo-setting glass ionomer cements promote variable biological responses of human dental pulp stem cells

Mar Collado-González; Miguel Ramón Pecci-Lloret; Christopher J. Tomás-Catalá; David García-Bernal; Ricardo E. Oñate-Sánchez; Carmen Llena; Leopoldo Forner; Vinicius Rosa; Francisco Javier Rodríguez-Lozano

OBJECTIVE To evaluate the in vitro cytotoxicity of Equia Forte (GC, Tokyo, Japan) and Ionostar Molar (Voco, Cuxhaven, Germany) on human dental pulp stem cells (hDPSCs). METHODS hDPSCs isolated from third molars were exposed to several dilutions of Equia Forte and Ionostar Molar eluates (1/1, 1/2 and 1/4). These eluates were obtained by storing material samples in respective cell culture medium for 24h (n=40). hDPSCs in basal growth culture medium were the control. Cell viability and cell migration assays were performed using the MTT and wound-healing assays, respectively. Also, induction of apoptosis and changes in cell phenotype were evaluated by flow cytometry. Changes in cell morphology were analysed by immunocytofluorescence staining. To evaluate cell attachment to the different materials, hDPSCs were directly seeded onto the material surfaces and analyzed by scanning electron microscopy (SEM). The chemical composition of the materials was determined by energy dispersive X-ray (EDX) and eluates were analyzed by inductively coupled plasma-mass spectrometry (ICP-MS). Statistical analysis was performed with analysis of variance (ANOVA) and Students t-test (α<0.05). RESULTS Undiluted Equia Forte extracts led to a similar cell proliferation rates than the control group from 72h onwards. There were no significance differences between Equia Forte and Ionostar Molar in terms of cell apoptosis and phenotype. However, in presence of Equia extracts the migration capacity of hDPSCs was higher than in presence of Ionostar Molar (p<0.05). Also, SEM studies showed a higher degree of cell attachment when Equia Forte extracts were used. Finally, EDX analysis pointed to different weight percentages of C, O and Ca ions in glass ionomer cements, while other elements such as La, Al, Si, W, Mo and F were also detected. SIGNIFICANCE In summary, Equia Forte promoted better biological responses in hDPSCs than Ionostar Molar.


International Endodontic Journal | 2017

Evaluation of cytocompatibility of calcium silicate-based endodontic sealers and their effects on the biological responses of mesenchymal dental stem cells

Francisco Javier Rodríguez-Lozano; David García-Bernal; Ricardo E. Oñate-Sánchez; P. S. Ortolani-Seltenerich; Leopoldo Forner; José M. Moraleda


International Endodontic Journal | 2017

Biocompatibility of three new calcium silicate-based endodontic sealers on human periodontal ligament stem cells

Mar Collado-González; David García-Bernal; Ricardo E. Oñate-Sánchez; P. S. Ortolani-Seltenerich; A. Lozano; Leopoldo Forner; Carmen Llena; Francisco Javier Rodríguez-Lozano


Journal of Cranio-maxillofacial Surgery | 2015

Cytoprotective effects of melatonin on zoledronic acid-treated human mesenchymal stem cells in vitro

Francisco Javier Rodríguez-Lozano; David García-Bernal; Maria de los Ángeles Ros-Roca; Maria del Carmen Algueró; Ricardo E. Oñate-Sánchez; Fabio Camacho-Alonso; José M. Moraleda


Oral Diseases | 2012

Mesenchymal stem cells and bisphosphonate‐related osteonecrosis of the jaw: the future?

M Gonzálvez‐García; Francisco Javier Rodríguez-Lozano; Villanueva; D Segarra‐Fenoll; Ma Rodríguez‐González; Ricardo E. Oñate-Sánchez; M Blanquer; L Meseguer‐Olmo; Jm Moraleda

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A. Lozano

University of Valencia

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