Ricardo Silva Araujo

Qualidade de um solo sob diferentes usos e sob Cerrado nativo

Sustainable soil use is becoming increasingly relevant given the increase of anthropic activities. In order to evaluate the soil quality, soil samples were collected from five areas: natural Cerrado (reference), native pasture, planted pasture, annual crops under conventional tillage and pine forest. The areas are located in the Federal District, Brazil, in a clayey Red Yellow Latosol (Acrustox). The following soil quality attributes were measured: bulk density, mechanical resistance to penetration, water infiltration rate, organic matter content, cation exchange capacity, carbon of microbial biomass and basal respiration. Based on the obtained data a comparative diagram was drawn and a soil quality index calculated for each type of use. The results showed a close inverse relationship between soil quality and intensity of use to which the areas were submitted. By analyzing these attributes and the establishment of a comparative model it was possible to evaluate the level of soil degradation caused by each type of use in each area.

Nitrogen fixation with the soybean crop in Brazil: Compatibility between seed treatment with fungicides and bradyrhizobial inoculants

Biological nitrogen fixation with the soybean crop can be improved by seed inoculation with superiorBradyrhizobium strains, but factors that reducethe population of inoculated bradyrhizobiaon the seedwill directly affect the efficiency of the process. Seed treatment with fungicides has been broadly practiced as cheap insurance against seed-and soil-borne pathogens, but toxicity of most fungicides to bradyrhizobia has often been underestimated. The compatibility between seed treatment with fungicides in single or mixed applications (including Benomyl, Captan, Carbendazin, Carboxin, Difenoconazole, Thiabendazole, Thiram, Tolylfluanid) and bradyrhizobial inoculants was examined in laboratory, greenhouse and field experiments during five crop seasons in Brazil. Bacterial survivalon the seeds was severely affected by all fungicides, resulting in mortalities of up to 62% after only 2 h and of 95% after 24 h. Fungicides also reduced nodule number, total N in grains and decreased yield by up to 17%. The toxic effects of fungicides were more drastic in sandy soils without soybean inoculation and cropping history, reducing nodulation by up to 87%, but were also important in areas with established populations of soybean bradyrhizobia. Therefore, fungicides should be used only when the seeds or soil are contaminated with pathogens, otherwise biological N2 fixation may be severely affected.

Estirpes de Rhizobium tropici na inoculação do feijoeiro

To evaluate five Rhizobium tropici strains and N fertilization on the productivity components of the common bean (Phaseolus vulgaris L.) crop, an experimental design of randomized blocks was used. The eight treatments consisted of five Rhizobium tropici strains (CIAT899; F35; F54; F81, and CM255), two controls without inoculation (30 kg ha-1 N with PK at sowing plus 30 kg ha-1 N as top dressing, and other without fertilization NPK) with the IAC Carioca cultivar and one non-nodulating cultivar (NORH54). Number of nodules per plant, shoot dry mass, leaf N content, number of pods per plant, number of grains per plant, number of grains per pod, 100 grain weight, and grain yield, were evaluated. The nodulating cultivar with inoculation or in soil with efficient native population can eliminate the N top dressing with no decrease in yield.

Maize growth promotion by inoculation with Azospirillum brasilense and metabolites of Rhizobium tropici enriched on lipo-chitooligosaccharides (LCOs)

There is an increasing interest in the development and use of inoculants carrying plant growth-promoting bacteria (PGPB) in crops of agronomic interest. The great majority of the inoculants commercialized worldwide contain rhizobia for legume crops, but the use of PGPB as Azospirillum spp. for non-legume is expanding, as well as of inoculants combining microorganisms and microbial metabolites. In this study we evaluated the effects of inoculants containing Azospirillum brasilense with or without metabolites of Rhizobium tropici strain CIAT 899 highly enriched in lipo-chitooligosaccharides (LCOs) in six field experiments performed for three summer crop seasons in Brazil with maize (Zea mays L.). Inoculants and metabolites were applied either at sowing by seed inoculation, or by leaf spray at the V3 stage of plant growth. Improvement in shoot dry weight (SDW) and total N accumulated in shoots (TNS) by single, but especially by dual inoculation was observed in some of the experiments. Statistically significant increases in grain yield in relation to the non-inoculated control were observed in five out of six experiments when maize was inoculated with Azospirillum supplied with enriched metabolites of R. tropici applied by seed or leaf spray inoculation. The results give strength to the development of a new generation of inoculants carrying microorganisms and microbial molecules.

In-furrow inoculation of soybean as alternative to fungicide and micronutrient seed treatment

SUMMARY Soybean is a major grain crop in Brazil, and yields can be considerably improved by inoculation with selected Bradyrhizobium strains. However, the incompatibility between inoculation and seed treatments with fungicides and micronutrients represents a major barrier to the achievement of high rates of biological N2 fixation. Inoculation practices that can alleviate the negative effects of agrochemicals must therefore be found and in-furrow inoculation seems to be an attractive alternative. This study reports the results of seven field experiments conducted in three growing seasons in Brazil; three in soils previously cropped with inoculated soybean (> 104 cells g -1 of soil of Bradyrhizobium), and four in areas where the crop was sown for the first time (< 10 2 cells g -1 of soil of Bradyrhizobium). The compatibility with fungicides and micronutrients was compared in seeds inoculated with peat or liquid inoculants, or treated with different doses of liquid inoculant in-furrow. In areas with established Bradyrhizobium populations, seed-applied agrochemicals did generally not affect nodulation, but also did not increase yields, while inoculation always increased N grain accumulation or yield, and N fertilizer decreased both nodulation and yield. Where soybean was sown for the first time, the seed treatment with agrochemicals affected nodulation when applied together with peat or liquid inoculant. In-furrow inoculation alleviated the effects of seed treatment with agrochemicals; the best performance was achieved with high Bradyrhizobium cell concentrations, with up to 2.5 million cells seed -1 .

Brazilian Trials to Evaluate the Effects of Soybean Reinoculation

Although Brazilian soils are very poor on N, the nitrogen fixation process is able to fully supply the N plant demands. Reinoculation with selected strains promotes increases in nodulation, nodule occupancy and yield.

Preinoculation of Soybean Seeds Treated with Agrichemicals up to 30 Days before Sowing: Technological Innovation for Large-Scale Agriculture

The cultivation of soybean in Brazil experienced an expressive growth in the last decades. Soybean is highly demanding on nitrogen (N) that must come from fertilizers or from biological fixation. The N supply to the soybean crop in Brazil relies on the inoculation with elite strains of Bradyrhizobium japonicum, B. elkanii, and B. diazoefficiens, which are able to fulfill the crops N requirements and enrich the soil for the following crop. The effectiveness of the association between N2-fixing bacteria and soybean plants depends on the efficacy of the inoculation process. Seed treatment with pesticides, especially fungicides or micronutrients, may rapidly kill the inoculated bacteria, affecting the establishment and outcome of the symbiosis. The development of technologies that allow inoculation to become a successful component of industrial seed treatment represents a valuable tool for the seed industry, as well as for the soybean crop worldwide. In this article, we report the results of new technologies, developed by the company Total Biotecnologia Indústria e Comércio S/A of Brazil, for preinoculation of soybean seeds with bradyrhizobia, in the presence of agrichemicals. Our results demonstrate improved bacterial survival for up to 30 days after inoculation, without compromising nodulation, N2-fixation, and yield in the field.