Richard F. Wright

Nitrogen deposition makes a minor contribution to carbon sequestration in temperate forests

Humans have altered global nitrogen cycling such that more atmospheric N2 is being converted (‘fixed’) into biologically reactive forms by anthropogenic activities than by all natural processes combined. In particular, nitrogen oxides emitted during fuel combustion and ammonia volatilized as a result of intensive agriculture have increased atmospheric nitrogen inputs (mostly NO3 and NH4) to temperate forests in the Northern Hemisphere. Because tree growth in northern temperate regions is typically nitrogen-limited, increased nitrogen deposition could have the effect of attenuating rising atmospheric CO2 by stimulating the accumulation of forest biomass. Forest inventories indicate that the carbon contents of northern forests have increased concurrently with nitrogen deposition since the 1950s. In addition, variations in atmospheric CO2 indicate a globally significant carbon sink in northern mid-latitude forest regions. It is unclear, however, whether elevated nitrogen deposition or other factors are the primary cause of carbon sequestration in northern forests. Here we use evidence from 15N-tracer studies in nine forests to show that elevated nitrogen deposition is unlikely to be a major contributor to the putative CO2 sink in forested northern temperature regions.

Nitrogen leaching from European forests in relation to nitrogen deposition

Abstract Forests in Europe currently receive inorganic nitrogen deposition (wet and dry) ranging from less than 1 to more than 75 kg N ha−1 year−1. A survey of nitrogen output from 65 forested plots and catchments throughout Europe indicated a three-stage response to these inputs. Below a deposition threshold of about 10 kg N ha−1 year−1 no significant nitrogen leaching occurred from the forests; at intermediate levels of 10–25 kg N ha−1 year−1 leaching occurred at some sites; above 25 kg ha−1 year−1 significant leaching occurred at all sites. NITREX sites followed this larger European pattern. Among 41 different variables tested in the survey, output-N (seepage or runoff) was most highly correlated with input-N (r2=0.69), followed by input-SO4, soil pH (negative correlation), percent slope, bedrock type and latitude (negative correlation). The latter three, however, were highly intercorrelated with input-N. A combination of input-N and soil pH explained 87% of the variability in output-N (N = 20) and predicted output-N successfully from NITREX sites and other European test sites.

Reversibility of acidification shown by whole-catchment experiments

Manipulation experiments are being conducted in Norway to examine the effects of drastic changes in precipitation chemistry on soil and surface-water acidification. At a clean area in western Norway, two pristine catchments are being acidified by addition of H2SO4 and H2SO4+HNO3, respectively. At an acidified catchment in southernmost Norway, ambient acid precipitation is excluded by means of a roof and clean precipitation added beneath.

Predicting the Effects of Atmospheric Nitrogen Deposition in Conifer Stands: Evidence from the NITREX Ecosystem-Scale Experiments

ABSTRACT The NITREX project, which encompasses seven ecosystem-scale experiments in coniferous forests at the plot or catchment level in northwestern Europe, investigates the effect of atmospheric nitrogen (N) deposition in coniferous forests. The common factor in all of the experiments is the experimentally controlled change in N input over a period of 4–5 years. Results indicate that the status and dynamics of the forest floor are key components in determining the response of forests to altered N inputs. An empirical relationship between the carbon–nitrogen (C/N) ratio of the forest floor and retention of incoming N provides a simply measured tool through which the likely timing and consequences of changes in atmospheric N deposition for fresh waters may be predicted. In the terrestrial ecosystem, a 50% increase in tree growth is observed following the experimental reduction of N and sulfur inputs in a highly N-saturated site, illustrating the damaging effects of acidifying pollutants to tree health in some locations. Few biotic responses to the experimental treatments were observed in other NITREX sites, but the rapid response of water quality to changes in N deposition, and the link to acidification in sensitive areas, highlight the need for N-emission controls, irrespective of the long-term effects on tree health. The observed changes in ecosystem function in response to the experimental treatments have been considered within the framework of the current critical-load approach and thus contribute to the formulation of environmental policy.

Introduction to the NITREX and EXMAN projects

Abstract European concern over the cause and consequences of forest decline, acidification of soils and surface waters, and the nutrient enrichment of terrestrial and aquatic ecosystems, led to the establishment of the NITREX (Nitrogen saturation experiments) and EXMAN (Experimental Manipulation of Forest Ecosystems in Europe) projects, two research networks of large-scale manipulation experiments under the auspices of the EU Commission of European Communities. NITREX comprises 10 experiments at 8 sites in 7 countries at which nitrogen is either added to or removed from ambient atmospheric deposition to simulate major changes in nitrogen deposition. EXMAN comprises experiments at 6 sites in 4 countries at which ambient atmospheric deposition is experimentally altered in chemical composition and/or quantity. The ultimate goal of this research is to contribute to the scientific basis required for the refinement of EU policy on atmospheric quality, and the legislation which will emanate from that policy.

The NITREX project: an introduction

Abstract NITREX is a consortium of European experiments in which nitrogen deposition is drastically changed for whole catchments or large forest stands at eight sites spanning the present-day gradient of nitrogen deposition across Europe. NITREX focuses on the impact of nitrogen deposition on forest ecosystems, in particular the factors and processes affecting ‘nitrogen saturation’. A large number of ecosystem processes and components are investigated as part of NITREX to identify and quantify the factors which lead to changes in nitrogen cycling and outputs due to experimental changes in nitrogen inputs. The data are used to develop and test several types of nitrogen models. NITREX is linked to several other European research projects, as well as to similar large-scale ecosystem experiments in North America.

Input-output budgets at the NITREX sites

The NITREX project entails large-scale manipulation of nitrogen deposition to whole, forested ecosystems at eight sites in Europe. Nitrogen is added at sites with low-to-intermediate ambient N deposition and removed at sites with high deposition. Changes in outputs of dissolved constituents reflect the integrated effects on ecosystem processes and changes in storage. At sites exhibiting clear symptoms of nitrogen saturation prior to treatment, the nitrate flux in leachate and runoff responded rapidly to changes in deposition. Reduced deposition gave immediate improvement in water quality. At sites with low nitrogen losses prior to treatment, the response to increased deposition was small and delayed. Together the results point to significant hysteresis in output response related to the nitrogen status of the ecosystem. The input-output budgets indicate that forest ecosystems require many years to adjust to changes in nitrogen deposition.

Nitrogen saturation experiments (NITREX) in coniferous forest ecosystems in Europe: a summary of results

The effect of changes in dissolved inorganic nitrogen (N) deposition on ecosystem functioning was investigated in the NITREX (NITRogen saturation EXperiments) project. Field-scale manipulation experiments were carried out over four to six years in seven coniferous forest ecosystems in northwestern Europe. At sites with low or moderate ambient N deposition, N was experimentally added to throughfall. At sites with high N deposition, N was removed from throughfall. We found that the capacity of the ecosystem to retain N was correlated to its internal N status. Some of the components of this N status like the N concentrations in foliage and forest floor are relatively easy to measure. The C/N ratio of the forest floor is especially closely related to the onset of nitrate leaching. Changes in N input may, in the long run, change the N status of an ecosystem due to for instance a decrease in C/N ratio in the forest floor. Decreased N input resulted in a rapid and large reduction in N concentration in drainage water. Significant improvement in tree nutritional status, tree growth, fine root biomass and diversity of ground vegetation and mycorrhizal fungi population were observed in one site only. The time period of four to six years of manipulated N deposition may have been too short for changes to be manifested in the other sites.

Impact of acid precipitation on freshwater ecosystems in Norway

Extensive studies of precipitation chemistry during the last 20 yr have clearly shown that highly polluted precipitation falls over large areas of Scandinavia, and that this pollution is increasing in severity and geographical extent. Precipitation in southern Norway, Sweden, and Finland contains large amounts of H+, SO=4, and NO−3 ions, along with heavy metals such as Cu, Zn, Cd, and Pb, that originate as air pollutants in the highly industrialized areas of Great Britain and central Europe and are transported over long distances to Scandinavia, where they are deposited in precipitation and dry-fallout.In Norway the acidification of fresh waters and accompanying decline and disappearance of fish populations were first reported in the 1920s, and since then in Sørlandet (southernmost Norway) the salmon have been eliminated from several rivers and hundreds of lakes have lost their fisheries.Justifiably, acid precipitation has become Norways number-one environmental problem, and in 1972 the government launched a major research project entitled ‘Acid precipitation — effects on forest and fish’, (the SNSF-project). Studies of freshwater ecosystems conducted by the SNSF-project include intensive research at 10 gauged watersheds and lake basins in critical acid-areas of southern Norway, extensive surveys of the geographical extent and severity of the problem over all of Norway, and field and laboratory experiments on the effect of acid waters on the growth and physiology of a variety of organisms.Large areas of western, southern, and eastern Norway have been adversely affected by acid precipitation. The pH of many lakes is below 5.0, and sulfate, rather than bicarbonate, is the major anion. Lakes in these areas are particularly vulnerable to acid precipitation because their watersheds are underlain by highly resistant bedrock with low Ca and Mg contents.Apart from the well-documented decline in fish populations, relatively little is known about the effects of acid precipitation on the biology of these aquatic ecosystems. Biological surveys indicate that low pH-values inhibit the decomposition of allochthonous organic matter, decrease the species number of phyto-and zooplankton and benthic invertebrates, and promote the growth of benthic mosses.Acid precipitation is affecting larger and larger areas of Norway. The source of the pollutants is industrial Europe, and the prognosis is a continued increase in fossil-fuel consumption. The short-term effects of the increasing acidity of freshwater ecosystems involve interference at every trophic level. The long-term impact may be quite drastic indeed.

NITREX: responses of coniferous forest ecosystems to experimentally changed deposition of nitrogen

Abstract In large regions of Europe and eastern North America atmospheric deposition of inorganic nitrogen compounds has greatly increased the natural external supply to forest ecosystems. This leads to nitrogen saturation, in which availability of inorganic nitrogen is in excess of biological demand and the ecosystem is unable to retain all incoming nitrogen. The large-scale experiments of the NITREX project (nitrogen saturation experiments) are designed to provide information regarding the patterns and rates of responses of coniferous forest ecosystems to increases in N deposition and the reversibility and recovery of impacted ecosystems following reductions in N deposition. The nitrogen input-output data from the NITREX sites are consistent with the general pattern of nitrogen fluxes from forest ecosystems in Europe. At annual inputs of less than about 10 kg ha−1 year−1, nearly all the nitrogen is retained and outputs are very small. At inputs above about 25 kg ha−1 year−1 outputs are substantial. In the range 10–25 kg ha−1 year−1 these forest ecosystems undergo a transition to nitrogen saturation. The 10 kg ha−1 year−1 apparently represents the minimum threshold for nitrogen saturation. The NITREX experiments indicate that nitrogen outputs respond markedly across the 10–25 kg ha−1 year−1 range of inputs. In contrast, the nutrient concentrations in foliage, a measure of tree response, is delayed by several years. Nitrogen saturation can apparently be induced or reversed within only a few years, at least with respect to the commonly used diagnostic of nitrogen saturation-nitrogen output in leachate or runoff.